DOI: 10.1128/aem.03218-13. Type IV Pilus Biogenesis, Twitching Motility, and DNA Uptake in Thermus thermophilus: Discrete Roles of Antagonistic ATPases PilF, PilT1, and PilT2

Type IV Pilus Biogenesis, Twitching Motility, and DNA Uptake in Thermus thermophilus: Discrete Roles of Antagonistic ATPases PilF, PilT1, and PilT2

10.1128/aem.03218-13

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT Natural transformation has a large impact on lateral gene flow and has contributed significantly to the ecological diversification and adaptation of bacterial species. Thermus thermophilus HB27 has emerged as the leading model organism for studies of DNA transporters in thermophilic bacteria. Recently, we identified a zinc-binding polymerization nucleoside triphosphatase (NTPase), PilF, which is essential for the transport of DNA through the outer membrane. Here, we present genetic evidence that PilF is also essential for the biogenesis of pili. One of the most challenging questions was whether T. thermophilus has any depolymerization NTPase acting as a counterplayer of PilF. We identified two depolymerization NTPases, PilT1 (TTC1621) and PilT2 (TTC1415), both of which are required for type IV pilus (T4P)-mediated twitching motility and adhesion but dispensable for natural transformation. This suggests that T4P dynamics are not required for natural transformation. The latter finding is consistent with our suggestion that in T. thermophilus , T4P and natural transformation are linked but distinct systems.

Bibliography

Salzer, R., Joos, F., & Averhoff, B. (2014). Type IV Pilus Biogenesis, Twitching Motility, and DNA Uptake in Thermus thermophilus: Discrete Roles of Antagonistic ATPases PilF, PilT1, and PilT2. Applied and Environmental Microbiology, 80(2), 644â652.

Authors 3

Ralf Salzer (first)
Friederike Joos (additional)
Beate Averhoff (additional)

References 60 Referenced 32

Dates

Type	When
Created	11 years, 9 months ago (Nov. 9, 2013, 12:07 a.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 3:06 p.m.)
Indexed	3 days, 9 hours ago (Aug. 29, 2025, 6:25 a.m.)
Issued	11 years, 7 months ago (Jan. 15, 2014)
Published	11 years, 7 months ago (Jan. 15, 2014)
Published Print	11 years, 7 months ago (Jan. 15, 2014)

Funders 0

None

BibTeX

@article{Salzer_2014, title={Type IV Pilus Biogenesis, Twitching Motility, and DNA Uptake in Thermus thermophilus: Discrete Roles of Antagonistic ATPases PilF, PilT1, and PilT2}, volume={80}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.03218-13}, DOI={10.1128/aem.03218-13}, number={2}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Salzer, Ralf and Joos, Friederike and Averhoff, Beate}, year={2014}, month=jan, pages={644–652} }

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