DOI: 10.1128/aem.02855-07. Transcription of <i>hupSL</i> in <i>Anabaena variabilis</i> ATCC 29413 Is Regulated by NtcA and Not by Hydrogen

Transcription of hupSL in Anabaena variabilis ATCC 29413 Is Regulated by NtcA and Not by Hydrogen

10.1128/aem.02855-07

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT Nitrogen-fixing cyanobacteria such as Anabaena variabilis ATCC 29413 use an uptake hydrogenase, encoded by hupSL , to recycle hydrogen gas that is produced as an obligate by-product of nitrogen fixation. The regulation of hupSL in A. variabilis is likely to differ from that of the closely related Anabaena sp. strain PCC 7120 because A. variabilis lacks the excision element-mediated regulation that characterizes hupSL regulation in strain PCC 7120. An analysis of the hupSL transcript in a nitrogenase mutant of A. variabilis that does not produce any detectable hydrogen indicated that neither nitrogen fixation nor hydrogen gas was required for the induction of hupSL . Furthermore, exogenous addition of hydrogen gas did not stimulate hupSL transcription. Transcriptional reporter constructs indicated that the accumulation of hupSL transcript after nitrogen step-down was restricted primarily to the microaerobic heterocysts. Anoxic conditions were not sufficient to induce hupSL transcription. The induction of hupSL after nitrogen step-down was reduced in a mutant in the global nitrogen regulator NtcA, but was not reduced in a mutant unable to form heterocysts. A consensus NtcA-binding site was identified upstream of hupSL , and NtcA was found to bind to this region. Thus, while neither hydrogen gas nor anoxia controlled the expression of hupSL , its expression was controlled by NtcA. Heterocyst differentiation was not required for hupSL induction in response to nitrogen step-down, but heterocyst-localized cues may add an additional level of regulation to hupSL .

Bibliography

Weyman, P. D., Pratte, B., & Thiel, T. (2008). Transcription of hupSL in Anabaena variabilis ATCC 29413 Is Regulated by NtcA and Not by Hydrogen. Applied and Environmental Microbiology, 74(7), 2103â2110.

Authors 3

Philip D. Weyman (first)
Brenda Pratte (additional)
Teresa Thiel (additional)

References 42 Referenced 28

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Dates

Type	When
Created	17 years, 6 months ago (Feb. 15, 2008, 9:20 p.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 11:46 a.m.)
Indexed	1 year, 11 months ago (Sept. 13, 2023, 2:53 p.m.)
Issued	17 years, 4 months ago (April 1, 2008)
Published	17 years, 4 months ago (April 1, 2008)
Published Print	17 years, 4 months ago (April 1, 2008)

Funders 0

None

BibTeX

@article{Weyman_2008, title={Transcription of hupSL in Anabaena variabilis ATCC 29413 Is Regulated by NtcA and Not by Hydrogen}, volume={74}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.02855-07}, DOI={10.1128/aem.02855-07}, number={7}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Weyman, Philip D. and Pratte, Brenda and Thiel, Teresa}, year={2008}, month=apr, pages={2103–2110} }

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