DOI: 10.1128/aem.02420-06. Effect of Protein, Polysaccharide, and Oxygen Concentration Profiles on Biofilm Cohesiveness

Effect of Protein, Polysaccharide, and Oxygen Concentration Profiles on Biofilm Cohesiveness

10.1128/aem.02420-06

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT It is important to control biofilm cohesiveness to optimize process performance. In this study, a membrane-aerated biofilm reactor inoculated with activated sludge was used to grow mixed-culture biofilms of different ages and thicknesses. The cohesions, or cohesive energy levels per unit volume of biofilm, based on a reproducible method using atomic force microscopy (F. Ahimou, M. J. Semmens, P. J. Novak, and G. Haugstad, Appl. Environ. Microbiol. 73:2897-2904, 2007), were determined at different locations within the depths of the biofilms. In addition, the protein and polysaccharide concentrations within the biofilm depths, as well as the dissolved oxygen (DO) concentration profiles within the biofilms, were measured. It was found that biofilm cohesion increased with depth but not with age. Level of biofilm cohesive energy per unit volume was strongly correlated with biofilm polysaccharide concentration, which increased with depth in the membrane-aerated biofilm. In a 12-day-old biofilm, DO also increased with depth and may therefore be linked to polysaccharide production. In contrast, protein concentration was relatively constant within the biofilm and did not appear to influence cohesion.

Bibliography

Ahimou, F., Semmens, M. J., Haugstad, G., & Novak, P. J. (2007). Effect of Protein, Polysaccharide, and Oxygen Concentration Profiles on Biofilm Cohesiveness. Applied and Environmental Microbiology, 73(9), 2905â2910.

Authors 4

Francois Ahimou (first)
Michael J. Semmens (additional)
Greg Haugstad (additional)
Paige J. Novak (additional)

References 36 Referenced 155

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10.1111/j.1574-6968.1998.tb13225.x
10.1002/bit.260370105
10.1128/iai.58.5.1344-1349.1990
Bishop, P. L., T. C. Zhang, and Y. C. Fu. 1995. Effects of biofilm structure, microbial distributions and mass transport on biodegradation processes. Water Sci. Technol.31:143-152. / Water Sci. Technol. (1995)
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10.1128/aem.59.4.1181-1186.1993
Flemming, H. C., J. Wingender, C. Mayer, V. Korstgens, and W. Borchard. 2000. Cohesiveness in biofilm matrix polymers, p. 87-105. In D. Allison, P. Gilbert, H. M. Lappin-Scott, and M. Wilson (ed.), Community structure and cooperation in biofilms. SGM symposium series, vol. 59. Cambridge University Press, Cambridge, United Kingdom. / Community structure and cooperation in biofilms. (2000)
Gaudy, A. F. 1962. Colorimetric determination of protein and carbohydrate. Ind. Water Wastes7:17-22. / Ind. Water Wastes (1962)
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Ohashi, A., T. Koyama, K. Syutsubo, and H. Harada. 1999. A novel method for evaluation of biofilm tensile strength resisting erosion. Water Sci. Technol.39:261-268. / Water Sci. Technol. (1999)
10.1128/aem.60.2.740-745.1994
Picioreanu, C., M. C. M. van Loosdrecht, and J. J. Heijnen. 1999. Discrete-differential modelling of biofilm structure. Water Sci. Technol.39:115-122. / Water Sci. Technol. (1999)
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Dates

Type	When
Created	18 years, 5 months ago (March 2, 2007, 9:13 p.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 11:33 a.m.)
Indexed	1 month, 1 week ago (July 24, 2025, 8:28 a.m.)
Issued	18 years, 4 months ago (May 1, 2007)
Published	18 years, 4 months ago (May 1, 2007)
Published Print	18 years, 4 months ago (May 1, 2007)

Funders 0

None

BibTeX

@article{Ahimou_2007, title={Effect of Protein, Polysaccharide, and Oxygen Concentration Profiles on Biofilm Cohesiveness}, volume={73}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.02420-06}, DOI={10.1128/aem.02420-06}, number={9}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Ahimou, Francois and Semmens, Michael J. and Haugstad, Greg and Novak, Paige J.}, year={2007}, month=may, pages={2905–2910} }

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