DOI: 10.1128/aem.01812-07. Novel Combination of Atomic Force Microscopy and Epifluorescence Microscopy for Visualization of Leaching Bacteria on Pyrite

Novel Combination of Atomic Force Microscopy and Epifluorescence Microscopy for Visualization of Leaching Bacteria on Pyrite

10.1128/aem.01812-07

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT Bioleaching of metal sulfides is an interfacial process comprising the interactions of attached bacterial cells and bacterial extracellular polymeric substances with the surface of a mineral sulfide. Such processes and the associated biofilms can be investigated at high spatial resolution using atomic force microscopy (AFM). Therefore, we visualized biofilms of the meso-acidophilic leaching bacterium Acidithiobacillus ferrooxidans strain A2 on the metal sulfide pyrite with a newly developed combination of AFM with epifluorescence microscopy (EFM). This novel system allowed the imaging of the same sample location with both instruments. The pyrite sample, as fixed on a shuttle stage, was transferred between AFM and EFM devices. By staining the bacterial DNA with a specific fluorescence dye, bacterial cells were labeled and could easily be distinguished from other topographic features occurring in the AFM image. AFM scanning in liquid caused deformation and detachment of cells, but scanning in air had no effect on cell integrity. In summary, we successfully demonstrate that the new microscopic system was applicable for visualizing bioleaching samples. Moreover, the combination of AFM and EFM in general seems to be a powerful tool for investigations of biofilms on opaque materials and will help to advance our knowledge of biological interfacial processes. In principle, the shuttle stage can be transferred to additional instruments, and combinations of AFM and EFM with other surface-analyzing devices can be proposed.

Bibliography

Mangold, S., Harneit, K., Rohwerder, T., Claus, G., & Sand, W. (2008). Novel Combination of Atomic Force Microscopy and Epifluorescence Microscopy for Visualization of Leaching Bacteria on Pyrite. Applied and Environmental Microbiology, 74(2), 410â415.

Authors 5

Stefanie Mangold (first)
Kerstin Harneit (additional)
Thore Rohwerder (additional)
Günter Claus (additional)
Wolfgang Sand (additional)

References 40 Referenced 65

10.1016/S0927-7765(01)00233-8
10.1103/PhysRevLett.56.930
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Nordstrom, D. K., and C. N. Alpers. 1999. Geochemistry of acid mine waters, p. 133-160. In G. S. Plumlee and M. J. Logsdon (ed.), The environmental geochemistry of mineral deposits. A. Processes, techniques, and health issues. The Society of Economic Geologists, Littleton, CO. / The environmental geochemistry of mineral deposits (1999)
Pace, D. L., R. E. Mielke, G. Southam, and T. L. Porter. 2005. Scanning force microscopy of the colonization and growth of A. ferrooxidans on the surface of pyrite minerals. Scanning27:136-140. / Scanning (2005)
10.1146/annurev.micro.56.012302.161052
10.1007/s00253-003-1448-7
Rohwerder, T., P. G. Jozsa, T. Gehrke, and W. Sand. 2002. Bioleaching, p. 632-641. In G. Bitton (ed.), Encyclopedia of environmental microbiology,vol. 2. John Wiley & Sons, Inc., New York, NY. / Encyclopedia of environmental microbiology (2002)
Rohwerder, T., and W. Sand. 2007. Mechanisms and biochemical fundamentals of bacterial metal sulfide oxidation, p. 35-58. In E. R. Donati and W. Sand (ed.), Microbial processing of metal sulfides. Springer, New York, NY. / Microbial processing of metal sulfides (2007)
10.1093/emboj/18.18.4981
Schippers, A. 2007. Microorganisms involved in bioleaching and nucleic acid-based molecular methods for their identification and quantification, p. 3-33. In E. R. Donati and W. Sand (ed.), Microbial processing of metal sulfides. Springer, New York, NY. / Microbial processing of metal sulfides (2007)
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10.1016/S0167-7012(02)00032-5
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Dates

Type	When
Created	17 years, 9 months ago (Nov. 26, 2007, 11:49 p.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 11:39 a.m.)
Indexed	4 months, 2 weeks ago (April 16, 2025, 7:26 a.m.)
Issued	17 years, 7 months ago (Jan. 15, 2008)
Published	17 years, 7 months ago (Jan. 15, 2008)
Published Print	17 years, 7 months ago (Jan. 15, 2008)

Funders 0

None

BibTeX

@article{Mangold_2008, title={Novel Combination of Atomic Force Microscopy and Epifluorescence Microscopy for Visualization of Leaching Bacteria on Pyrite}, volume={74}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.01812-07}, DOI={10.1128/aem.01812-07}, number={2}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Mangold, Stefanie and Harneit, Kerstin and Rohwerder, Thore and Claus, Günter and Sand, Wolfgang}, year={2008}, month=jan, pages={410–415} }

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