DOI: 10.1128/aem.00819-10. Repression of Antibiotic Production and Sporulation in<i>Streptomyces coelicolor</i>by Overexpression of a TetR Family Transcriptional Regulator

Repression of Antibiotic Production and Sporulation inStreptomyces coelicolorby Overexpression of a TetR Family Transcriptional Regulator

10.1128/aem.00819-10

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACTThe overexpression of a regulatory gene of the TetR family (SCO3201) originating either fromStreptomyces lividansor fromStreptomyces coelicolorwas shown to strongly repress antibiotic production (calcium-dependent antibiotic [CDA], undecylprodigiosin [RED], and actinorhodin [ACT]) ofS. coelicolorand of theppkmutant strain ofS. lividans. Curiously, the overexpression of this gene also had a strong inhibitory effect on the sporulation process ofS. coelicolorbut not on that ofS. lividans. SCO3201 was shown to negatively regulate its own transcription, and its DNA binding motif was found to overlap its −35 promoter sequence. The interruption of this gene inS. lividansorS. coelicolordid not lead to any obvious phenotypes, indicating that when overexpressed SCO3201 likely controls the expression of target genes of other TetR regulators involved in the regulation of the metabolic and morphological differentiation process inS. coelicolor. The direct and functional interaction of SCO3201 with the promoter region ofscbA, a gene under the positive control of the TetR-like regulator, ScbR, was indeed demonstrated byin vitroas well asin vivoapproaches.

Bibliography

Xu, D., Seghezzi, N., Esnault, C., & Virolle, M.-J. (2010). Repression of Antibiotic Production and Sporulation inStreptomyces coelicolorby Overexpression of a TetR Family Transcriptional Regulator. Applied and Environmental Microbiology, 76(23), 7741â7753.

Authors 4

Delin Xu (first)
Nicolas Seghezzi (additional)
Catherine Esnault (additional)
Marie-Joelle Virolle (additional)

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Dates

Type	When
Created	14 years, 10 months ago (Oct. 8, 2010, 9:12 p.m.)
Deposited	1 year, 5 months ago (March 31, 2024, 4:11 a.m.)
Indexed	2 months, 3 weeks ago (June 9, 2025, 1:02 a.m.)
Issued	14 years, 9 months ago (Dec. 1, 2010)
Published	14 years, 9 months ago (Dec. 1, 2010)
Published Print	14 years, 9 months ago (Dec. 1, 2010)

Funders 0

None

BibTeX

@article{Xu_2010, title={Repression of Antibiotic Production and Sporulation inStreptomyces coelicolorby Overexpression of a TetR Family Transcriptional Regulator}, volume={76}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.00819-10}, DOI={10.1128/aem.00819-10}, number={23}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Xu, Delin and Seghezzi, Nicolas and Esnault, Catherine and Virolle, Marie-Joelle}, year={2010}, month=dec, pages={7741–7753} }

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