DOI: 10.1128/aem.00743-08. Characterization of an <i>Escherichia coli</i> O157:H7 O-Antigen Deletion Mutant and Effect of the Deletion on Bacterial Persistence in the Mouse Intestine and Colonization at the Bovine Terminal Rectal Mucosa

Characterization of an Escherichia coli O157:H7 O-Antigen Deletion Mutant and Effect of the Deletion on Bacterial Persistence in the Mouse Intestine and Colonization at the Bovine Terminal Rectal Mucosa

10.1128/aem.00743-08

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT Escherichia coli O157:H7 causes hemorrhagic colitis and the life-threatening hemolytic-uremic syndrome in humans and transiently colonizes healthy cattle at the terminal rectal mucosa. To investigate the role of the O antigen in persistence and colonization in the animal host, we generated an E. coli O157:H7 mutant defective in the synthesis of the lipopolysaccharide side chain (O antigen) by deletion of a putative perosamine synthetase gene ( per ) in the rfb cluster. The lack of O antigen was confirmed by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and anti-O157 antibody. The growth rate and cell membrane permeability of the Δ per mutant were similar to the growth rate and cell membrane permeability of the wild type. Changes in membrane and secreted proteins were observed, but the expression of intimin, EspA, and EspB, implicated in bacterial intestinal colonization, was not altered, as determined by immunoblotting and reverse transcription-PCR. Similar to other O-antigen deletion mutants, the Δ per mutant was pleiotropic for autoaggregation and motility (it was FliC negative as determined by immunoblotting and flagellum negative as determined by electron microscopy). The abilities of the mutant and the wild type to persist in the murine intestine and to colonize the bovine terminal rectal mucosa were compared. Mice fed the Δ per mutant shed lower numbers of bacteria ( P < 0.05) over a shorter time than mice fed the wild-type or complemented strain. After rectal application in steers, lower numbers of the Δ per mutant than of the wild type colonized the rectoanal junction mucosa, and the duration of the colonization was shorter ( P < 0.05). Our previous work showed that flagella do not influence E. coli O157:H7 colonization at the bovine terminal rectal mucosa, so the current findings suggest that the O antigen contributes to efficient bovine colonization.

Bibliography

Sheng, H., Lim, J. Y., Watkins, M. K., Minnich, S. A., & Hovde, C. J. (2008). Characterization of an Escherichia coli O157:H7 O-Antigen Deletion Mutant and Effect of the Deletion on Bacterial Persistence in the Mouse Intestine and Colonization at the Bovine Terminal Rectal Mucosa. Applied and Environmental Microbiology, 74(16), 5015â5022.

Authors 5

Haiqing Sheng (first)
Ji Youn Lim (additional)
Maryann K. Watkins (additional)
Scott A. Minnich (additional)
Carolyn J. Hovde (additional)

References 41 Referenced 44

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Bettelheim, K. A. 2000. Role of non-O157 VTEC. Symp. Ser. Soc. Appl. Microbiol.29:38S-50S. / Symp. Ser. Soc. Appl. Microbiol. (2000)
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Dates

Type	When
Created	17 years, 2 months ago (June 13, 2008, 10:15 p.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 11:46 a.m.)
Indexed	2 months, 3 weeks ago (June 6, 2025, 3:30 p.m.)
Issued	17 years ago (Aug. 15, 2008)
Published	17 years ago (Aug. 15, 2008)
Published Print	17 years ago (Aug. 15, 2008)

Funders 0

None

BibTeX

@article{Sheng_2008, title={Characterization of an Escherichia coli O157:H7 O-Antigen Deletion Mutant and Effect of the Deletion on Bacterial Persistence in the Mouse Intestine and Colonization at the Bovine Terminal Rectal Mucosa}, volume={74}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.00743-08}, DOI={10.1128/aem.00743-08}, number={16}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Sheng, Haiqing and Lim, Ji Youn and Watkins, Maryann K. and Minnich, Scott A. and Hovde, Carolyn J.}, year={2008}, month=aug, pages={5015–5022} }

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