DOI: 10.1128/aac.6.6.680. 3-N Enzymatic Acetylation of Gentamicin, Tobramycin, and Kanamycin by <i>Escherichia coli</i> Carrying an R Factor

3-N Enzymatic Acetylation of Gentamicin, Tobramycin, and Kanamycin by Escherichia coli Carrying an R Factor

10.1128/aac.6.6.680

Crossref journal-article

American Society for Microbiology

Antimicrobial Agents and Chemotherapy (235)

Abstract

A new R factor-mediated gentamicin acetyltransferase obtained from a clinical isolate of gentamicin-, tobramycin-, and kanamycin-resistant Klebsiella was characterized. R factor R 176 was transferred to Escherichia coli K-12 LA 290 from a Klebsiella . Resistance transfer, minimal inhibitory concentrations of various aminoglycoside antibiotics, enzymatic inactivation, and the isoelectric point of the enzyme obtained were determined. We found that R factor R 176 could govern the enzymatic 3-N acetylation of gentamicin, tobramycin, and kanamycin, and isoelectric point determinations suggested that the enzymes governed by R 176 and R 135 are different. The genes responsible for resistance to gentamicin, tobramycin, and kanamycin govern the synthesis of an isoenzyme of AAC(3)I, which we propose to call AAC(3)II.

Bibliography

Le Goffic, F., Martel, A., & Witchitz, J. (1974). 3-N Enzymatic Acetylation of Gentamicin, Tobramycin, and Kanamycin by Escherichia coli Carrying an R Factor. Antimicrobial Agents and Chemotherapy, 6(6), 680â684.

Authors 3

François Le Goffic (first)
Annie Martel (additional)
Janine Witchitz (additional)

References 0 Referenced 19

None

Dates

Type	When
Created	13 years, 2 months ago (June 27, 2012, 7:57 p.m.)
Deposited	3 years, 6 months ago (Feb. 21, 2022, 6:12 p.m.)
Indexed	6 days, 20 hours ago (Aug. 29, 2025, 6:15 a.m.)
Issued	50 years, 9 months ago (Dec. 1, 1974)
Published	50 years, 9 months ago (Dec. 1, 1974)
Published Print	50 years, 9 months ago (Dec. 1, 1974)

Funders 0

None

BibTeX

@article{Le_Goffic_1974, title={3-N Enzymatic Acetylation of Gentamicin, Tobramycin, and Kanamycin by Escherichia coli Carrying an R Factor}, volume={6}, ISSN={1098-6596}, url={http://dx.doi.org/10.1128/aac.6.6.680}, DOI={10.1128/aac.6.6.680}, number={6}, journal={Antimicrobial Agents and Chemotherapy}, publisher={American Society for Microbiology}, author={Le Goffic, François and Martel, Annie and Witchitz, Janine}, year={1974}, month=dec, pages={680–684} }

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