Abstract
ABSTRACT A screening assay to test the inducing capacity of macrolides by fusing the attenuator of the inducible erm (B) gene from Streptococcus pneumoniae HM28 with the gfpmut1 gene has been designed. Fluorescence was detected under UV light around disks impregnated with inducer macrolides (erythromycin or azithromycin) but not with noninducer ketolides. Induction could be quantified by fluorometry.
References
19
Referenced
15
10.1128/AAC.41.10.2149
10.1093/jac/40.1.85
10.1046/j.1469-0691.2000.00185.x
-
Comité de l'Antibiogramme de la Société Française de Microbiologie. 1996. Technical recommendations for in vitro susceptibility testing. Clin. Microbiol. Infect.2(Suppl. 1):11-25.
(
10.1111/j.1469-0691.1996.tb00871.x
) / Clin. Microbiol. Infect. (1996) 10.1016/S0960-894X(99)00534-X
-
Denis, A., F. Bretin, C. Fromentin, A. Bonnet, G. Piltan, A. Bonnefoy, and C. Agouridas. 2000. Synthesis and antibacterial activity of 2-halogeno, 2-methyl and 2,3 enol-ether ketolides. Bioorg. Med. Chem. Lett.10:2019-2022.
(
10.1016/S0960-894X(00)00392-9
) / Bioorg. Med. Chem. Lett. (2000) 10.1046/j.1365-2958.2000.01841.x
10.1128/AAC.33.7.1058
10.1038/nbt0998-862
10.1128/jb.154.3.1252-1262.1983
10.1128/AAC.35.7.1267
10.1016/0147-619X(87)90068-0
10.1128/AAC.43.12.2823
10.1128/AAC.42.6.1392
10.1093/jac/43.4.559
10.1093/nar/18.14.4296
10.1128/AAC.39.3.577
10.1128/AAC.39.4.797
10.1089/mdr.1999.5.183
Dates
Type | When |
---|---|
Created | 23 years ago (July 28, 2002, 7:07 p.m.) |
Deposited | 3 years, 6 months ago (Feb. 21, 2022, 12:33 p.m.) |
Indexed | 1 year, 1 month ago (July 9, 2024, 7:37 p.m.) |
Issued | 23 years, 1 month ago (July 1, 2002) |
Published | 23 years, 1 month ago (July 1, 2002) |
Published Print | 23 years, 1 month ago (July 1, 2002) |
@article{Clarebout_2002, title={Fluorescence Assay for Studying the Ability of Macrolides To Induce Production of Ribosomal Methylase}, volume={46}, ISSN={1098-6596}, url={http://dx.doi.org/10.1128/aac.46.7.2269-2272.2002}, DOI={10.1128/aac.46.7.2269-2272.2002}, number={7}, journal={Antimicrobial Agents and Chemotherapy}, publisher={American Society for Microbiology}, author={Clarebout, Gervais and Leclercq, Roland}, year={2002}, month=jul, pages={2269–2272} }