Evernimicin Binds Exclusively to the 50S Ribosomal Subunit and Inhibits Translation in Cell-Free Systems Derived from both Gram-Positive and Gram-Negative Bacteria
10.1128/aac.44.5.1121-1126.2000
Crossref journal-article
American Society for Microbiology
Antimicrobial Agents and Chemotherapy (235)
Abstract

ABSTRACT Evernimicin (SCH 27899) is a new antibiotic with activity against a wide spectrum of gram-positive bacteria and activity against some gram-negative bacteria. Previous metabolic labeling studies indicated that evernimicin specifically inhibited protein synthesis in Staphylococcus aureus . Using a susceptible Escherichia coli strain, we demonstrated that evernimicin also inhibited protein synthesis in E. coli . In cell-free translation assays with extracts from either E. coli or S. aureus , evernimicin had a 50% inhibitory concentration of approximately 125 nM. In contrast, cell-free systems derived from wheat germ and rabbit reticulocytes were inhibited only by very high levels of evernimicin. Evernimicin did not promote transcript misreading. [ 14 C]evernimicin specifically bound to the 50S subunit from E. coli . Nonlinear regression analysis of binding data generated with 70S ribosomes from E. coli and S. aureus and 50S subunits from E. coli returned dissociation constants of 84, 86, and 160 nM, respectively. In binding experiments, performed in the presence of excess quantities of a selection of antibiotics known to bind to the 50S subunit, only the structurally similar drug avilamycin blocked binding of [ 14 C]evernimicin to ribosomes.

Bibliography

McNicholas, P. M., Najarian, D. J., Mann, P. A., Hesk, D., Hare, R. S., Shaw, K. J., & Black, T. A. (2000). Evernimicin Binds Exclusively to the 50S Ribosomal Subunit and Inhibits Translation in Cell-Free Systems Derived from both Gram-Positive and Gram-Negative Bacteria. Antimicrobial Agents and Chemotherapy, 44(5), 1121–1126.

Authors 7
  1. Paul M. McNicholas (first)
  2. David J. Najarian (additional)
  3. Paul A. Mann (additional)
  4. David Hesk (additional)
  5. Roberta S. Hare (additional)
  6. Karen J. Shaw (additional)
  7. Todd A. Black (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 27, 2002, 6:06 a.m.)
Deposited 3 years, 6 months ago (Feb. 21, 2022, 11:54 a.m.)
Indexed 1 month, 3 weeks ago (July 11, 2025, 6:19 a.m.)
Issued 25 years, 4 months ago (May 1, 2000)
Published 25 years, 4 months ago (May 1, 2000)
Published Print 25 years, 4 months ago (May 1, 2000)
Funders 0

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@article{McNicholas_2000, title={Evernimicin Binds Exclusively to the 50S Ribosomal Subunit and Inhibits Translation in Cell-Free Systems Derived from both Gram-Positive and Gram-Negative Bacteria}, volume={44}, ISSN={1098-6596}, url={http://dx.doi.org/10.1128/aac.44.5.1121-1126.2000}, DOI={10.1128/aac.44.5.1121-1126.2000}, number={5}, journal={Antimicrobial Agents and Chemotherapy}, publisher={American Society for Microbiology}, author={McNicholas, Paul M. and Najarian, David J. and Mann, Paul A. and Hesk, David and Hare, Roberta S. and Shaw, Karen J. and Black, Todd A.}, year={2000}, month=may, pages={1121–1126} }