DOI: 10.1128/aac.44.12.3357-3363.2000. Effect of Subinhibitory Antibiotic Concentrations on Polysaccharide Intercellular Adhesin Expression in Biofilm-Forming <i>Staphylococcus epidermidis</i>

Effect of Subinhibitory Antibiotic Concentrations on Polysaccharide Intercellular Adhesin Expression in Biofilm-Forming Staphylococcus epidermidis

10.1128/aac.44.12.3357-3363.2000

Crossref journal-article

American Society for Microbiology

Antimicrobial Agents and Chemotherapy (235)

Abstract

ABSTRACT Biofilm production is an important step in the pathogenesis of Staphylococcus epidermidis polymer-associated infections and depends on the expression of the ica ADBC operon leading to the synthesis of a polysaccharide intercellular adhesin. A chromosomally encoded reporter gene fusion between the ica promoter and the beta-galactosidase gene lac Z from Escherichia coli was constructed and used to investigate the influence of both environmental factors and subinhibitory concentrations of different antibiotics on ica expression in S. epidermidis . It was shown that S. epidermidis biofilm formation is induced by external stress (i.e., high temperature and osmolarity). Subinhibitory concentrations of tetracycline and the semisynthetic streptogramin antibiotic quinupristin-dalfopristin were found to enhance ica expression 9- to 11-fold, whereas penicillin, oxacillin, chloramphenicol, clindamycin, gentamicin, ofloxacin, vancomycin, and teicoplanin had no effect on ica expression. A weak (i.e., 2.5-fold) induction of ica expression was observed for subinhibitory concentrations of erythromycin. The results were confirmed by Northern blot analyses of ica transcription and quantitative analyses of biofilm formation in a colorimetric assay.

Bibliography

Rachid, S., Ohlsen, K., Witte, W., Hacker, J., & Ziebuhr, W. (2000). Effect of Subinhibitory Antibiotic Concentrations on Polysaccharide Intercellular Adhesin Expression in Biofilm-Forming Staphylococcus epidermidis. Antimicrobial Agents and Chemotherapy, 44(12), 3357â3363.

Authors 5

Shwan Rachid (first)
Knut Ohlsen (additional)
Wolfgang Witte (additional)
Jörg Hacker (additional)
Wilma Ziebuhr (additional)

References 56 Referenced 302

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:06 a.m.)
Deposited	3 years, 6 months ago (Feb. 21, 2022, 11:23 a.m.)
Indexed	4 days, 1 hour ago (Sept. 3, 2025, 6:47 a.m.)
Issued	24 years, 9 months ago (Dec. 1, 2000)
Published	24 years, 9 months ago (Dec. 1, 2000)
Published Print	24 years, 9 months ago (Dec. 1, 2000)

Funders 0

None

BibTeX

@article{Rachid_2000, title={Effect of Subinhibitory Antibiotic Concentrations on Polysaccharide Intercellular Adhesin Expression in Biofilm-Forming Staphylococcus epidermidis}, volume={44}, ISSN={1098-6596}, url={http://dx.doi.org/10.1128/aac.44.12.3357-3363.2000}, DOI={10.1128/aac.44.12.3357-3363.2000}, number={12}, journal={Antimicrobial Agents and Chemotherapy}, publisher={American Society for Microbiology}, author={Rachid, Shwan and Ohlsen, Knut and Witte, Wolfgang and Hacker, Jörg and Ziebuhr, Wilma}, year={2000}, month=dec, pages={3357–3363} }

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