DOI: 10.1128/aac.38.12.2775. Nitric oxide donor-mediated killing of bioluminescent Escherichia coli

Nitric oxide donor-mediated killing of bioluminescent Escherichia coli

10.1128/aac.38.12.2775

Crossref journal-article

American Society for Microbiology

Antimicrobial Agents and Chemotherapy (235)

Abstract

The antimicrobial activities of two nitric oxide-releasing compounds against Escherichia coli were investigated by using recombinant E. coli cloned with a luciferase gene from Pyrophorus plagiophthalamus. Since luciferase uses intracellular ATP to generate visible light which can be measured from living cells in real time, we wanted to compare the extent to which cell viability parallels light emission. Results from luminescence measurements and CFU counts were in good agreement, and the decrease in light emission was shown to provide a rapid and more sensitive indication of cytotoxicity.

Bibliography

Virta, M., Karp, M., & Vuorinen, P. (1994). Nitric oxide donor-mediated killing of bioluminescent Escherichia coli. Antimicrobial Agents and Chemotherapy, 38(12), 2775â2779.

Authors 3

M Virta (first)
M Karp (additional)
P Vuorinen (additional)

References 0 Referenced 26

None

Dates

Type	When
Created	13 years, 2 months ago (June 27, 2012, 8:05 p.m.)
Deposited	3 years, 6 months ago (Feb. 21, 2022, 10:35 a.m.)
Indexed	1 year, 10 months ago (Oct. 28, 2023, 6:56 a.m.)
Issued	30 years, 9 months ago (Dec. 1, 1994)
Published	30 years, 9 months ago (Dec. 1, 1994)
Published Print	30 years, 9 months ago (Dec. 1, 1994)

Funders 0

None

BibTeX

@article{Virta_1994, title={Nitric oxide donor-mediated killing of bioluminescent Escherichia coli}, volume={38}, ISSN={1098-6596}, url={http://dx.doi.org/10.1128/aac.38.12.2775}, DOI={10.1128/aac.38.12.2775}, number={12}, journal={Antimicrobial Agents and Chemotherapy}, publisher={American Society for Microbiology}, author={Virta, M and Karp, M and Vuorinen, P}, year={1994}, month=dec, pages={2775–2779} }

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