DOI: 10.1128/aac.37.9.1896. Characterization of the chromosomal aac(6')-Ii gene specific for Enterococcus faecium

Characterization of the chromosomal aac(6')-Ii gene specific for Enterococcus faecium

10.1128/aac.37.9.1896

Crossref journal-article

American Society for Microbiology

Antimicrobial Agents and Chemotherapy (235)

Abstract

Chromosomal gene aac(6')-Ii of Enterococcus faecium CIP 54-32, encoding a 6'-N-aminoglycoside acetyltransferase was characterized. The gene was identified as a coding sequence of 549 bp corresponding to a protein with a calculated mass of 20,666 Da. Analysis of the sequence of the deduced protein suggested that it was the second member of a subfamily of AAC(6')-I enzymes. Insertional inactivation of aac(6')-Ii led to aminoglycoside susceptibility of CIP 54-32, suggesting that this gene plays a role in resistance to AAC(6')-I substrates. The gene was detected by DNA hybridization in all 26 strains of E. faecium tested but not in 44 other enterococci of 13 species. These data suggest that the aac(6')-Ii gene is species specific and may be used to identify E. faecium.

Bibliography

Costa, Y., Galimand, M., Leclercq, R., Duval, J., & Courvalin, P. (1993). Characterization of the chromosomal aac(6â)-Ii gene specific for Enterococcus faecium. Antimicrobial Agents and Chemotherapy, 37(9), 1896â1903.

Authors 5

Y Costa (first)
M Galimand (additional)
R Leclercq (additional)
J Duval (additional)
P Courvalin (additional)

References 0 Referenced 154

None

Dates

Type	When
Created	13 years, 2 months ago (June 27, 2012, 8:05 p.m.)
Deposited	3 years, 6 months ago (Feb. 21, 2022, 10:36 a.m.)
Indexed	4 days, 5 hours ago (Aug. 26, 2025, 2:34 a.m.)
Issued	31 years, 11 months ago (Sept. 1, 1993)
Published	31 years, 11 months ago (Sept. 1, 1993)
Published Print	31 years, 11 months ago (Sept. 1, 1993)

Funders 0

None

BibTeX

@article{Costa_1993, title={Characterization of the chromosomal aac(6’)-Ii gene specific for Enterococcus faecium}, volume={37}, ISSN={1098-6596}, url={http://dx.doi.org/10.1128/aac.37.9.1896}, DOI={10.1128/aac.37.9.1896}, number={9}, journal={Antimicrobial Agents and Chemotherapy}, publisher={American Society for Microbiology}, author={Costa, Y and Galimand, M and Leclercq, R and Duval, J and Courvalin, P}, year={1993}, month=sep, pages={1896–1903} }

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