DOI: 10.1128/aac.32.10.1494. Cloning and expression of methicillin resistance from Staphylococcus epidermidis in Staphylococcus carnosus

Cloning and expression of methicillin resistance from Staphylococcus epidermidis in Staphylococcus carnosus

10.1128/aac.32.10.1494

Crossref journal-article

American Society for Microbiology

Antimicrobial Agents and Chemotherapy (235)

Abstract

A 6.2-kilobase chromosomal DNA fragment from a methicillin-resistant Staphylococcus epidermidis strain was cloned into Staphylococcus carnosus by using staphylococcal plasmid pCA44 as the vector. The recombinant plasmid obtained, pBBB21, conferred methicillin resistance on its host and was responsible for the synthesis of a low-affinity penicillin-binding protein (PBP), PBP 2'. PBP 2' determined by the S. epidermidis DNA and expressed as a membrane-bound PBP in S. carnosus reacted with monoclonal antibodies directed against PBP 2' of Staphylococcus aureus origin, and the cloned S. epidermidis DNA hybridized to the methicillin (mec)-specific DNA from S. aureus. These findings point to a common origin of the methicillin resistance determinant in staphylococci.

Bibliography

Tesch, W., StrÃ¤ssle, A., Berger-BÃ¤chi, B., OâHara, D., Reynolds, P., & Kayser, F. H. (1988). Cloning and expression of methicillin resistance from Staphylococcus epidermidis in Staphylococcus carnosus. Antimicrobial Agents and Chemotherapy, 32(10), 1494â1499.

Authors 6

W Tesch (first)
A Strässle (additional)
B Berger-Bächi (additional)
D O'Hara (additional)
P Reynolds (additional)
F H Kayser (additional)

References 0 Referenced 58

None

Dates

Type	When
Created	13 years, 2 months ago (June 27, 2012, 8:04 p.m.)
Deposited	3 years, 6 months ago (Feb. 21, 2022, 10:02 a.m.)
Indexed	1 year, 7 months ago (Jan. 15, 2024, 12:23 a.m.)
Issued	36 years, 10 months ago (Oct. 1, 1988)
Published	36 years, 10 months ago (Oct. 1, 1988)
Published Print	36 years, 10 months ago (Oct. 1, 1988)

Funders 0

None

BibTeX

@article{Tesch_1988, title={Cloning and expression of methicillin resistance from Staphylococcus epidermidis in Staphylococcus carnosus}, volume={32}, ISSN={1098-6596}, url={http://dx.doi.org/10.1128/aac.32.10.1494}, DOI={10.1128/aac.32.10.1494}, number={10}, journal={Antimicrobial Agents and Chemotherapy}, publisher={American Society for Microbiology}, author={Tesch, W and Strässle, A and Berger-Bächi, B and O’Hara, D and Reynolds, P and Kayser, F H}, year={1988}, month=oct, pages={1494–1499} }

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