DOI: 10.1128/aac.20.6.787. Virus inhibition assay for measurement of acyclovir levels in human plasma and urine

Virus inhibition assay for measurement of acyclovir levels in human plasma and urine

10.1128/aac.20.6.787

Crossref journal-article

American Society for Microbiology

Antimicrobial Agents and Chemotherapy (235)

Abstract

A simple microplate virus inhibition assay which measures the levels of acyclovir in plasma and urine samples from patients was developed. The assay is based upon the inhibition of the cytopathic effect of herpes simplex virus type 1 on human fibroblast cells. The extent of inhibition of virus cytopathic effect, caused by dilutions of samples from patients, allowed determination of acyclovir concentrations to be made. The assay, which measured biological activity, could detect acyclovir levels of greater than or equal to 1.0 microM. Peak and valley levels measured in plasma samples from two patients were comparable to values obtained by radioimmunoassay. The assay is simple, rapid, and quantitative, and it can be adapted to routine use for a large number of specimens.

Bibliography

Moore, D. F., Taylor, S. C., & Bryson, Y. J. (1981). Virus inhibition assay for measurement of acyclovir levels in human plasma and urine. Antimicrobial Agents and Chemotherapy, 20(6), 787â792.

Authors 3

D F Moore (first)
S C Taylor (additional)
Y J Bryson (additional)

References 0 Referenced 7

None

Dates

Type	When
Created	13 years, 2 months ago (June 27, 2012, 8:03 p.m.)
Deposited	3 years, 6 months ago (Feb. 21, 2022, 9:10 a.m.)
Indexed	1 day, 17 hours ago (Sept. 4, 2025, 9:48 a.m.)
Issued	43 years, 9 months ago (Dec. 1, 1981)
Published	43 years, 9 months ago (Dec. 1, 1981)
Published Print	43 years, 9 months ago (Dec. 1, 1981)

Funders 0

None

BibTeX

@article{Moore_1981, title={Virus inhibition assay for measurement of acyclovir levels in human plasma and urine}, volume={20}, ISSN={1098-6596}, url={http://dx.doi.org/10.1128/aac.20.6.787}, DOI={10.1128/aac.20.6.787}, number={6}, journal={Antimicrobial Agents and Chemotherapy}, publisher={American Society for Microbiology}, author={Moore, D F and Taylor, S C and Bryson, Y J}, year={1981}, month=dec, pages={787–792} }

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