DOI: 10.1128/aac.01152-09. Triclosan Resistance of <i>Pseudomonas aeruginosa</i> PAO1 Is Due to FabV, a Triclosan-Resistant Enoyl-Acyl Carrier Protein Reductase

Triclosan Resistance of Pseudomonas aeruginosa PAO1 Is Due to FabV, a Triclosan-Resistant Enoyl-Acyl Carrier Protein Reductase

10.1128/aac.01152-09

Crossref journal-article

American Society for Microbiology

Antimicrobial Agents and Chemotherapy (235)

Abstract

ABSTRACT Triclosan, a very widely used biocide, specifically inhibits fatty acid synthesis by inhibition of enoyl-acyl carrier protein (ACP) reductase. Escherichia coli FabI is the prototypical triclosan-sensitive enoyl-ACP reductase, and E. coli is extremely sensitive to the biocide. However, other bacteria are resistant to triclosan, because they encode triclosan-resistant enoyl-ACP reductase isozymes. In contrast, the triclosan resistance of Pseudomonas aeruginosa PAO1 has been attributed to active efflux of the compound (R. Chuanchuen, R. R. Karkhoff-Schweizer, and H. P. Schweizer, Am. J. Infect. Control 31: 124-127, 2003). We report that P. aeruginosa contains two enoyl-ACP reductase isozymes, the previously characterized FabI homologue plus a homologue of FabV, a triclosan-resistant enoyl-ACP reductase recently demonstrated in Vibrio cholerae . By deletion of the genes encoding P. aeruginosa FabI and FabV, we demonstrated that FabV confers triclosan resistance on P. aeruginosa . Upon deletion of the fabV gene, the mutant strain became extremely sensitive to triclosan (>2,000-fold more sensitive than the wild-type strain), whereas the mutant strain lacking FabI remained completely resistant to the biocide.

Bibliography

Zhu, L., Lin, J., Ma, J., Cronan, J. E., & Wang, H. (2010). Triclosan Resistance of Pseudomonas aeruginosa PAO1 Is Due to FabV, a Triclosan-Resistant Enoyl-Acyl Carrier Protein Reductase. Antimicrobial Agents and Chemotherapy, 54(2), 689â698.

Authors 5

Lei Zhu (first)
Jinshui Lin (additional)
Jincheng Ma (additional)
John E. Cronan (additional)
Haihong Wang (additional)

References 46 Referenced 173

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Dates

Type	When
Created	15 years, 9 months ago (Nov. 23, 2009, 10:44 p.m.)
Deposited	3 years, 6 months ago (Feb. 21, 2022, 2:55 p.m.)
Indexed	2 weeks, 2 days ago (Aug. 12, 2025, 5:40 p.m.)
Issued	15 years, 6 months ago (Feb. 1, 2010)
Published	15 years, 6 months ago (Feb. 1, 2010)
Published Print	15 years, 6 months ago (Feb. 1, 2010)

Funders 0

None

BibTeX

@article{Zhu_2010, title={Triclosan Resistance of Pseudomonas aeruginosa PAO1 Is Due to FabV, a Triclosan-Resistant Enoyl-Acyl Carrier Protein Reductase}, volume={54}, ISSN={1098-6596}, url={http://dx.doi.org/10.1128/aac.01152-09}, DOI={10.1128/aac.01152-09}, number={2}, journal={Antimicrobial Agents and Chemotherapy}, publisher={American Society for Microbiology}, author={Zhu, Lei and Lin, Jinshui and Ma, Jincheng and Cronan, John E. and Wang, Haihong}, year={2010}, month=feb, pages={689–698} }

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