Cryo-EM with sub–1 Å specimen movement
10.1126/science.abb7927
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Going for the gold Single-particle cryogenic electron microscopy (cryo-EM) has become a go-to technique for structural biologists. Although data-processing and reconstruction methods have improved, innovations in sample preparation and data collection are essential to reliably achieve high-resolution reconstructions while also reducing the amount of time required per structure. Naydenova et al. tackled the issue of electron beam–induced particle movement, a major source of information loss, by designing a gold sample support that prevents buckling of the extremely thin layer of ice in which the particles are suspended (see Perspective by Rapp and Carragher). The negligible particle displacement permits extrapolation to “zero exposure” structure factors, revealing features typically lost in cryo-EM structures. Far fewer particles per unit resolution are required, which greatly accelerates structure determination, especially at high resolution. Science , this issue p. 223 ; see also p. 171

Bibliography

Naydenova, K., Jia, P., & Russo, C. J. (2020). Cryo-EM with sub–1 Å specimen movement. Science, 370(6513), 223–226.

Authors 3
  1. Katerina Naydenova (first)
  2. Peipei Jia (additional)
  3. Christopher J. Russo (additional)
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Dates
Type When
Created 4 years, 10 months ago (Oct. 16, 2020, 3:50 p.m.)
Deposited 1 year, 7 months ago (Jan. 15, 2024, 6:21 p.m.)
Indexed 1 week, 1 day ago (Aug. 12, 2025, 6:06 p.m.)
Issued 4 years, 10 months ago (Oct. 9, 2020)
Published 4 years, 10 months ago (Oct. 9, 2020)
Published Print 4 years, 10 months ago (Oct. 9, 2020)
Funders 2
  1. Australian Nanotechnology Network 10.13039/100013474

    Region: Oceania

    pri (Other non-profit organizations)

    Labels4
    1. The Australian Nanotechnology Network
    2. Aus Nanotech Network
    3. ANN
    4. AUSNANO
  2. Medical Research Council 10.13039/501100000265

    Region: Europe

    gov (National government)

    Labels3
    1. Medical Research Council (United Kingdom)
    2. UK Medical Research Council
    3. MRC
    Awards1
    1. MC_UP_120117

@article{Naydenova_2020, title={Cryo-EM with sub–1 Å specimen movement}, volume={370}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.abb7927}, DOI={10.1126/science.abb7927}, number={6513}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Naydenova, Katerina and Jia, Peipei and Russo, Christopher J.}, year={2020}, month=oct, pages={223–226} }