DOI: 10.1126/science.aam6892. Atomic structure of the human cytomegalovirus capsid with its securing tegument layer of pp150

Atomic structure of the human cytomegalovirus capsid with its securing tegument layer of pp150

10.1126/science.aam6892

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Strong under pressureHuman cytomegalovirus (HCMV) is a member of the herpesvirus family that can cause life-threatening infections in those who are immunocompromised. HCMV encodes a genome that is about 50% larger than that of herpes simplex virus 1 (the virus that causes cold sores), but these two viruses have similar-sized capsids. Yuet al.used cryo–electron microscopy to determine the structure of the HCMV capsid to 3.9-Å resolution. This is the first high-resolution capsid structure from the herpesvirus family. It reveals extensive interactions that stabilize the capsid to withstand the high pressure that comes from accommodating such a large genome.Science, this issue p.eaam6892

Bibliography

Yu, X., Jih, J., Jiang, J., & Zhou, Z. H. (2017). Atomic structure of the human cytomegalovirus capsid with its securing tegument layer of pp150. Science, 356(6345).

Authors 4

Xuekui Yu (first)
Jonathan Jih (additional)
Jiansen Jiang (additional)
Z. Hong Zhou (additional)

References 66 Referenced 99

Dates

Type	When
Created	8 years, 1 month ago (July 12, 2017, 5:45 p.m.)
Deposited	2 months, 1 week ago (June 22, 2025, 12:14 a.m.)
Indexed	3 weeks, 2 days ago (Aug. 6, 2025, 9:06 a.m.)
Issued	8 years, 1 month ago (June 30, 2017)
Published	8 years, 1 month ago (June 30, 2017)
Published Print	8 years, 1 month ago (June 30, 2017)

Funders 6

National Science Foundation 10.13039/100000001
Region: Americas
gov (National government)
Labels4
1. U.S. National Science Foundation
2. NSF
3. US NSF
4. USA NSF
Awards2
1. DBI-1338135
2. award312681
National Institutes of Health 10.13039/100000002
Region: Americas
gov (National government)
Labels3
1. Institutos Nacionales de la Salud
2. US National Institutes of Health
3. NIH
Awards2
1. award312676
2. GM071940
National Institutes of Health 10.13039/100000002
Region: Americas
gov (National government)
Labels3
1. Institutos Nacionales de la Salud
2. US National Institutes of Health
3. NIH
Awards2
1. award312677
2. DE025567
National Institutes of Health 10.13039/100000002
Region: Americas
gov (National government)
Labels3
1. Institutos Nacionales de la Salud
2. US National Institutes of Health
3. NIH
Awards2
1. AI094386
2. award312678
National Institutes of Health 10.13039/100000002
Region: Americas
gov (National government)
Labels3
1. Institutos Nacionales de la Salud
2. US National Institutes of Health
3. NIH
Awards2
1. award312679
2. 1S10RR23057
National Institutes of Health 10.13039/100000002
Region: Americas
gov (National government)
Labels3
1. Institutos Nacionales de la Salud
2. US National Institutes of Health
3. NIH
Awards2
1. award312680
2. 1U24GM116792

BibTeX

@article{Yu_2017, title={Atomic structure of the human cytomegalovirus capsid with its securing tegument layer of pp150}, volume={356}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.aam6892}, DOI={10.1126/science.aam6892}, number={6345}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Yu, Xuekui and Jih, Jonathan and Jiang, Jiansen and Zhou, Z. Hong}, year={2017}, month=jun }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        6
      ]
    ],
    "date-time": "2025-08-06T13:06:48Z",
    "timestamp": 1754485608073,
    "version": "3.41.2"
  },
  "reference-count": 66,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "6345",
  "funder": [
    {
      "DOI": "10.13039/100000001",
      "name": "National Science Foundation",
      "doi-asserted-by": "publisher",
      "award": [
        "award312681",
        "DBI-1338135"
      ],
      "id": [
        {
          "id": "10.13039/100000001",
          "id-type": "DOI",
          "asserted-by": "publisher"
        }
      ]
    },
    {
      "DOI": "10.13039/100000002",
      "name": "National Institutes of Health",
      "doi-asserted-by": "publisher",
      "award": [
        "award312676",
        "GM071940"
      ],
      "id": [
        {
          "id": "10.13039/100000002",
          "id-type": "DOI",
          "asserted-by": "publisher"
        }
      ]
    },
    {
      "DOI": "10.13039/100000002",
      "name": "National Institutes of Health",
      "doi-asserted-by": "publisher",
      "award": [
        "award312677",
        "DE025567"
      ],
      "id": [
        {
          "id": "10.13039/100000002",
          "id-type": "DOI",
          "asserted-by": "publisher"
        }
      ]
    },
    {
      "DOI": "10.13039/100000002",
      "name": "National Institutes of Health",
      "doi-asserted-by": "publisher",
      "award": [
        "award312678",
        "AI094386"
      ],
      "id": [
        {
          "id": "10.13039/100000002",
          "id-type": "DOI",
          "asserted-by": "publisher"
        }
      ]
    },
    {
      "DOI": "10.13039/100000002",
      "name": "National Institutes of Health",
      "doi-asserted-by": "publisher",
      "award": [
        "award312679",
        "1S10RR23057"
      ],
      "id": [
        {
          "id": "10.13039/100000002",
          "id-type": "DOI",
          "asserted-by": "publisher"
        }
      ]
    },
    {
      "DOI": "10.13039/100000002",
      "name": "National Institutes of Health",
      "doi-asserted-by": "publisher",
      "award": [
        "award312680",
        "1U24GM116792"
      ],
      "id": [
        {
          "id": "10.13039/100000002",
          "id-type": "DOI",
          "asserted-by": "publisher"
        }
      ]
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2017,
        6,
        30
      ]
    ]
  },
  "abstract": "<jats:title>Strong under pressure</jats:title><jats:p>Human cytomegalovirus (HCMV) is a member of the herpesvirus family that can cause life-threatening infections in those who are immunocompromised. HCMV encodes a genome that is about 50% larger than that of herpes simplex virus 1 (the virus that causes cold sores), but these two viruses have similar-sized capsids. Yu<jats:italic>et al.</jats:italic>used cryo\u2013electron microscopy to determine the structure of the HCMV capsid to 3.9-\u00c5 resolution. This is the first high-resolution capsid structure from the herpesvirus family. It reveals extensive interactions that stabilize the capsid to withstand the high pressure that comes from accommodating such a large genome.</jats:p><jats:p><jats:italic>Science</jats:italic>, this issue p.<jats:related-article xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"doi\" issue=\"6345\" page=\"eaam6892\" related-article-type=\"in-this-issue\" vol=\"356\" xlink:href=\"10.1126/science.aam6892\">eaam6892</jats:related-article></jats:p>",
  "DOI": "10.1126/science.aam6892",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2017,
        7,
        12
      ]
    ],
    "date-time": "2017-07-12T21:45:10Z",
    "timestamp": 1499895910000
  },
  "source": "Crossref",
  "is-referenced-by-count": 99,
  "title": "Atomic structure of the human cytomegalovirus capsid with its securing tegument layer of pp150",
  "prefix": "10.1126",
  "volume": "356",
  "author": [
    {
      "ORCID": "https://orcid.org/0000-0002-4434-2040",
      "authenticated-orcid": false,
      "given": "Xuekui",
      "family": "Yu",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095-7364, USA."
        },
        {
          "name": "California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA 90095-7364, USA."
        }
      ]
    },
    {
      "ORCID": "https://orcid.org/0000-0001-5169-0919",
      "authenticated-orcid": false,
      "given": "Jonathan",
      "family": "Jih",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095-7364, USA."
        },
        {
          "name": "California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA 90095-7364, USA."
        }
      ]
    },
    {
      "given": "Jiansen",
      "family": "Jiang",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA 90095-7364, USA."
        }
      ]
    },
    {
      "ORCID": "https://orcid.org/0000-0002-8373-4717",
      "authenticated-orcid": false,
      "given": "Z. Hong",
      "family": "Zhou",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095-7364, USA."
        },
        {
          "name": "California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA 90095-7364, USA."
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_3_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.2174/1568005310101020179"
    },
    {
      "key": "e_1_3_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00006454-200512000-00016"
    },
    {
      "key": "e_1_3_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00005792-198405000-00002"
    },
    {
      "key": "e_1_3_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1056/NEJM199312233292604"
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1086/320902"
    },
    {
      "key": "e_1_3_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.3947/ic.2013.45.3.260"
    },
    {
      "key": "e_1_3_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/clinids/10.Supplement_3.S460"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/rmv.655"
    },
    {
      "key": "e_1_3_2_10_2",
      "unstructured": "E. S. Mocarski C. T. Courcelle in Fields Virology vol. 2 D. M. Knipe et al . Eds. (Lippincott Williams and Wilkins 2001) pp. 2629\u20132674."
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00711459"
    },
    {
      "key": "e_1_3_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/978-1-60761-786-0_16"
    },
    {
      "key": "e_1_3_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature10003"
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature12519"
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1099/vir.0.18606-0"
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1999.3344"
    },
    {
      "key": "e_1_3_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ja404008r"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.75.15.6865-6873.2001"
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jsb.2011.03.006"
    },
    {
      "key": "e_1_3_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1371/journal.ppat.1003525"
    },
    {
      "key": "e_1_3_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0076-6879(10)82015-4"
    },
    {
      "key": "e_1_3_2_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jsb.2011.05.004"
    },
    {
      "key": "e_1_3_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1249410"
    },
    {
      "key": "e_1_3_2_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature13567"
    },
    {
      "key": "e_1_3_2_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.cell.2016.05.040"
    },
    {
      "key": "e_1_3_2_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.1300601110"
    },
    {
      "key": "e_1_3_2_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.7554/eLife.07901"
    },
    {
      "key": "e_1_3_2_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.aaf5316"
    },
    {
      "key": "e_1_3_2_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nsmb.3212"
    },
    {
      "key": "e_1_3_2_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.1420317112"
    },
    {
      "key": "e_1_3_2_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.73.4.3210-3218.1999"
    },
    {
      "key": "e_1_3_2_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.289.5487.2129"
    },
    {
      "key": "e_1_3_2_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0502164102"
    },
    {
      "key": "e_1_3_2_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.7554/eLife.01299"
    },
    {
      "key": "e_1_3_2_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.79.23.14967-14970.2005"
    },
    {
      "key": "e_1_3_2_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/cdg086"
    },
    {
      "key": "e_1_3_2_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1993.1406"
    },
    {
      "key": "e_1_3_2_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1103/PhysRevE.72.021917"
    },
    {
      "key": "e_1_3_2_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nsb1195-1026"
    },
    {
      "key": "e_1_3_2_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.77.4.2730-2735.2003"
    },
    {
      "key": "e_1_3_2_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jmb.2010.01.043"
    },
    {
      "key": "e_1_3_2_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.00837-11"
    },
    {
      "key": "e_1_3_2_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.01509-14"
    },
    {
      "key": "e_1_3_2_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/gku634"
    },
    {
      "key": "e_1_3_2_45_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.00457-06"
    },
    {
      "key": "e_1_3_2_46_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.241486298"
    },
    {
      "key": "e_1_3_2_47_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.1233721100"
    },
    {
      "key": "e_1_3_2_48_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.3934/biophy.2015.2.200"
    },
    {
      "key": "e_1_3_2_49_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.str.2014.04.003"
    },
    {
      "key": "e_1_3_2_50_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.str.2008.05.016"
    },
    {
      "key": "e_1_3_2_51_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jsbi.2000.4314"
    },
    {
      "key": "e_1_3_2_52_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jsb.2005.03.010"
    },
    {
      "key": "e_1_3_2_53_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth.2472"
    },
    {
      "key": "e_1_3_2_54_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1047-8477(03)00069-8"
    },
    {
      "key": "e_1_3_2_55_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1047-8477(02)00014-X"
    },
    {
      "key": "e_1_3_2_56_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jsbi.1999.4174"
    },
    {
      "key": "e_1_3_2_57_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jsb.2010.05.006"
    },
    {
      "key": "e_1_3_2_58_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jmb.2003.07.013"
    },
    {
      "key": "e_1_3_2_59_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nmeth.2727"
    },
    {
      "key": "e_1_3_2_60_2",
      "doi-asserted-by": "crossref",
      "unstructured": "Y. Wan W. Chiu Z. H. Zhou in 2004 International Conference on Communications Circuits and Systems (Institute of Electrical and Electronics Engineers 2004) pp. 960\u2013964.",
      "DOI": "10.1109/ICCCAS.2004.1346339"
    },
    {
      "key": "e_1_3_2_61_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/B978-0-12-386507-6.00001-4"
    },
    {
      "key": "e_1_3_2_62_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0304-3991(99)00120-5"
    },
    {
      "key": "e_1_3_2_63_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jcc.20084"
    },
    {
      "key": "e_1_3_2_64_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0907444904019158"
    },
    {
      "key": "e_1_3_2_65_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/gkv332"
    },
    {
      "key": "e_1_3_2_66_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nprot.2015.053"
    },
    {
      "key": "e_1_3_2_67_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0907444909052925"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1126/science.aam6892",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.aam6892",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2025,
        6,
        22
      ]
    ],
    "date-time": "2025-06-22T04:14:06Z",
    "timestamp": 1750565646000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.aam6892"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2017,
        6,
        30
      ]
    ]
  },
  "references-count": 66,
  "journal-issue": {
    "issue": "6345",
    "published-print": {
      "date-parts": [
        [
          2017,
          6,
          30
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.aam6892"
  ],
  "URL": "http://dx.doi.org/10.1126/science.aam6892",
  "relation": {
    "has-review": [
      {
        "id-type": "doi",
        "id": "10.3410/f.727762354.793534506",
        "asserted-by": "object"
      }
    ]
  },
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        2017,
        6,
        30
      ]
    ]
  },
  "article-number": "eaam6892"
}