DOI: 10.1126/science.aad2001. Architecture of the type IVa pilus machine

Architecture of the type IVa pilus machine

10.1126/science.aad2001

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

How the bacterial pilus works Many bacteria, including important pathogens, move by projecting grappling-hook–like extensions called type IV pili from their cell bodies. After these pili attach to other cells or objects in their environment, the bacteria retract the pili to pull themselves forward. Chang et al. used electron cryotomography of intact cells to image the protein machines that extend and retract the pili, revealing where each protein component resides. Putting the known structures of the individual proteins in place like pieces of a three-dimensional puzzle revealed insights into how the machine works, including evidence that ATP hydrolysis by cytoplasmic motors rotates a membrane-embedded adaptor that slips pilin subunits back and forth from the membrane onto the pilus. Science , this issue p. 10.1126/science.aad2001

Bibliography

Chang, Y.-W., Rettberg, L. A., Treuner-Lange, A., Iwasa, J., SÃ¸gaard-Andersen, L., & Jensen, G. J. (2016). Architecture of the type IVa pilus machine. Science, 351(6278).

Authors 6

Yi-Wei Chang (first)
Lee A. Rettberg (additional)
Anke Treuner-Lange (additional)
Janet Iwasa (additional)
Lotte Søgaard-Andersen (additional)
Grant J. Jensen (additional)

References 99 Referenced 378

Dates

Type	When
Created	9 years, 5 months ago (March 10, 2016, 2:36 p.m.)
Deposited	1 year, 7 months ago (Jan. 15, 2024, 12:25 p.m.)
Indexed	3 weeks, 4 days ago (July 30, 2025, 9:05 a.m.)
Issued	9 years, 5 months ago (March 11, 2016)
Published	9 years, 5 months ago (March 11, 2016)
Published Print	9 years, 5 months ago (March 11, 2016)

Funders 4

NIH 10.13039/100000002 National Institutes of Health
Region: Americas
gov (National government)
Labels3
1. Institutos Nacionales de la Salud
2. US National Institutes of Health
3. NIH
Awards1
1. R01 GM094800B
Howard Hughes Medical Institute 10.13039/100000011
Region: Americas
pri (Research institutes and centers)
Labels2
1. Howard Hughes Medical Institute Inc
2. HHMI
Max Planck Society 10.13039/501100004189 Max-Planck-Gesellschaft
Region: Europe
gov (Research institutes and centers)
Labels4
1. Max Planck Society for the Advancement of Science
2. Max-Planck-Gesellschaft zur Förderung der Wissenschaften
3. Max Planck Society
4. MPG
Deutsche Forschungsgemeinschaft 10.13039/501100001659
Region: Europe
gov (National government)
Labels3
1. German Research Association
2. German Research Foundation
3. DFG

BibTeX

@article{Chang_2016, title={Architecture of the type IVa pilus machine}, volume={351}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.aad2001}, DOI={10.1126/science.aad2001}, number={6278}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Chang, Yi-Wei and Rettberg, Lee A. and Treuner-Lange, Anke and Iwasa, Janet and Søgaard-Andersen, Lotte and Jensen, Grant J.}, year={2016}, month=mar }

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