DOI: 10.1126/science.aaa4938. Cryo-EM shows the polymerase structures and a nonspooled genome within a dsRNA virus

Cryo-EM shows the polymerase structures and a nonspooled genome within a dsRNA virus

10.1126/science.aaa4938

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Caught in the act of copying The genomes of double-stranded RNA (dsRNA) viruses consist of about a dozen dsRNA segments enclosed by a protein coat. Inside the host cell, the coat remains intact, and the dsRNAs have to replicate within the coat. Liu and Cheng used cryo–electron microscopy of cypovirus particles to catch the dsRNAs in the act of being copied. The structures revealed that the RNA formed a liquid-crystalline array on which viral enzymes carry out multiple rounds of transcription to replicate the viral genome. Science , this issue p. 1347

Bibliography

Liu, H., & Cheng, L. (2015). Cryo-EM shows the polymerase structures and a nonspooled genome within a dsRNA virus. Science, 349(6254), 1347â1350.

Authors 2

Hongrong Liu (first)
Lingpeng Cheng (additional)

References 41 Referenced 85

Dates

Type	When
Created	9 years, 11 months ago (Sept. 18, 2015, 1:49 a.m.)
Deposited	1 year, 7 months ago (Jan. 15, 2024, 12:22 p.m.)
Indexed	1 month ago (July 25, 2025, 6:34 a.m.)
Issued	9 years, 11 months ago (Sept. 18, 2015)
Published	9 years, 11 months ago (Sept. 18, 2015)
Published Print	9 years, 11 months ago (Sept. 18, 2015)

Funders 4

National Natural Science Foundation of China 10.13039/501100001809
Region: Asia
gov (National government)
Labels11
1. Chinese National Science Foundation
2. Natural Science Foundation of China
3. National Science Foundation of China
4. NNSF of China
5. NSF of China
6. 国家自然科学基金委员会
7. National Nature Science Foundation of China
8. Guójiā Zìrán Kēxué Jījīn Wěiyuánhuì
9. NSFC
10. NNSF
11. NNSFC
Awards4
1. 31170697
2. 31000333
3. 91230116
4. 31370736
Program for New Century Excellent Talents 10.13039/501100004602 Program for New Century Excellent Talents in University
Region: Asia
gov (Local government)
Labels3
1. New Century Excellent Talents Program
2. Program for New Century Excellent Talents
3. NCET
Awards1
1. NCET-13-0787
National Basic Research Program of China 10.13039/501100012166 National Key Research and Development Program of China
Region: Asia
gov (National government)
Labels16
1. 重点基础研究发展计划
2. National Basic Research Program of China (973 Program)
3. Special Fund for the National Key Research and Development Plan
4. China National Key Research and Development Plan Project
5. National Key Research and Development of China
6. National Key Research and Development Program
7. National Key R&D Program of China
8. National Key R&D Programmes of China
9. China's National Key R&D Programmes
10. National Basic Research Program of China
11. 973 Program
12. National Program on Key Basic Research Project (973 Program)
13. National Plan on Key Basic Research and Development
14. National Basic Research Program
15. NKRDPC
16. NKPs
Awards1
1. 2010CB912403
Hunan Provincial NSF
Awards1
1. 13JJ1017

BibTeX

@article{Liu_2015, title={Cryo-EM shows the polymerase structures and a nonspooled genome within a dsRNA virus}, volume={349}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.aaa4938}, DOI={10.1126/science.aaa4938}, number={6254}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Liu, Hongrong and Cheng, Lingpeng}, year={2015}, month=sep, pages={1347–1350} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        25
      ]
    ],
    "date-time": "2025-07-25T10:34:39Z",
    "timestamp": 1753439679765,
    "version": "3.37.3"
  },
  "reference-count": 41,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "6254",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2016,
            9,
            18
          ]
        ],
        "date-time": "2016-09-18T00:00:00Z",
        "timestamp": 1474156800000
      },
      "content-version": "vor",
      "delay-in-days": 366,
      "URL": "http://www.sciencemag.org/about/science-licenses-journal-article-reuse"
    }
  ],
  "funder": [
    {
      "DOI": "10.13039/501100001809",
      "name": "National Natural Science Foundation of China",
      "doi-asserted-by": "publisher",
      "award": [
        "91230116",
        "31170697",
        "31370736",
        "31000333"
      ],
      "id": [
        {
          "id": "10.13039/501100001809",
          "id-type": "DOI",
          "asserted-by": "publisher"
        }
      ]
    },
    {
      "DOI": "10.13039/501100004602",
      "name": "Program for New Century Excellent Talents",
      "doi-asserted-by": "publisher",
      "award": [
        "NCET-13-0787"
      ],
      "id": [
        {
          "id": "10.13039/501100004602",
          "id-type": "DOI",
          "asserted-by": "publisher"
        }
      ]
    },
    {
      "DOI": "10.13039/501100012166",
      "name": "National Basic Research Program of China",
      "doi-asserted-by": "crossref",
      "award": [
        "2010CB912403"
      ],
      "id": [
        {
          "id": "10.13039/501100012166",
          "id-type": "DOI",
          "asserted-by": "crossref"
        }
      ]
    },
    {
      "name": "Hunan Provincial NSF",
      "award": [
        "13JJ1017"
      ]
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2015,
        9,
        18
      ]
    ]
  },
  "abstract": "<jats:title>Caught in the act of copying</jats:title>\n          <jats:p>The genomes of double-stranded RNA (dsRNA) viruses consist of about a dozen dsRNA segments enclosed by a protein coat. Inside the host cell, the coat remains intact, and the dsRNAs have to replicate within the coat. Liu and Cheng used cryo\u2013electron microscopy of cypovirus particles to catch the dsRNAs in the act of being copied. The structures revealed that the RNA formed a liquid-crystalline array on which viral enzymes carry out multiple rounds of transcription to replicate the viral genome.</jats:p>\n          <jats:p>\n            <jats:italic>Science</jats:italic>\n            , this issue p.\n            <jats:related-article xmlns:xlink=\"http://www.w3.org/1999/xlink\" ext-link-type=\"doi\" issue=\"6254\" page=\"1347\" related-article-type=\"in-this-issue\" vol=\"349\" xlink:href=\"10.1126/science.aaa4938\">1347</jats:related-article>\n          </jats:p>",
  "DOI": "10.1126/science.aaa4938",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2015,
        9,
        18
      ]
    ],
    "date-time": "2015-09-18T05:49:19Z",
    "timestamp": 1442555359000
  },
  "page": "1347-1350",
  "source": "Crossref",
  "is-referenced-by-count": 85,
  "title": "Cryo-EM shows the polymerase structures and a nonspooled genome within a dsRNA virus",
  "prefix": "10.1126",
  "volume": "349",
  "author": [
    {
      "given": "Hongrong",
      "family": "Liu",
      "sequence": "first",
      "affiliation": [
        {
          "name": "College of Physics and Information Science, Hunan Normal University, Changsha, Hunan 410081, China."
        }
      ]
    },
    {
      "given": "Lingpeng",
      "family": "Cheng",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "School of Life Sciences, Tsinghua University, Beijing 100084, China."
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_3_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nsb0297-118"
    },
    {
      "key": "e_1_3_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.virusres.2003.12.002"
    },
    {
      "key": "e_1_3_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(02)01110-8"
    },
    {
      "key": "e_1_3_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.str.2008.09.006"
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.122.5.1023"
    },
    {
      "key": "e_1_3_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/386088a0"
    },
    {
      "key": "e_1_3_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/386091a0"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35010041"
    },
    {
      "key": "e_1_3_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature03056"
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/emboj.2009.109"
    },
    {
      "key": "e_1_3_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature09546"
    },
    {
      "key": "e_1_3_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1187433"
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35065653"
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.1014995108"
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.1200206109"
    },
    {
      "key": "e_1_3_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nsb1009"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0042-6822(80)90047-1"
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "crossref",
      "unstructured": "P. G. de Gennes J. Prost The Physics of Liquid Crystals (Oxford Univ. Press Oxford 1993).",
      "DOI": "10.1093/oso/9780198520245.001.0001"
    },
    {
      "key": "e_1_3_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80758-8"
    },
    {
      "key": "e_1_3_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.98.4.1381"
    },
    {
      "key": "e_1_3_2_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M205964200"
    },
    {
      "key": "e_1_3_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M308637200"
    },
    {
      "key": "e_1_3_2_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.virusres.2003.12.005"
    },
    {
      "key": "e_1_3_2_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0611599104"
    },
    {
      "key": "e_1_3_2_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jmb.2014.03.013"
    },
    {
      "key": "e_1_3_2_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0969-2126(03)00091-1"
    },
    {
      "key": "e_1_3_2_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0042-6822(80)90187-7"
    },
    {
      "key": "e_1_3_2_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jsbi.1999.4174"
    },
    {
      "key": "e_1_3_2_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1047-8477(02)00014-X"
    },
    {
      "key": "e_1_3_2_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jsb.2007.09.016"
    },
    {
      "key": "e_1_3_2_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81773-0"
    },
    {
      "key": "e_1_3_2_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nature04487"
    },
    {
      "key": "e_1_3_2_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jsb.2005.03.009"
    },
    {
      "key": "e_1_3_2_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jsbi.2001.4379"
    },
    {
      "key": "e_1_3_2_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jsb.2006.08.021"
    },
    {
      "key": "e_1_3_2_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jsbi.1996.0009"
    },
    {
      "key": "e_1_3_2_38_2",
      "unstructured": "J. M. Cowley Diffraction Physics (North-Holland Personal Library Elsevier Amsterdam ed. 3 1995)."
    },
    {
      "key": "e_1_3_2_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.ultramic.2013.06.004"
    },
    {
      "key": "e_1_3_2_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0907444904019158"
    },
    {
      "key": "e_1_3_2_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1107/S0907444909052925"
    },
    {
      "key": "e_1_3_2_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jcc.20084"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.aaa4938",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        15
      ]
    ],
    "date-time": "2024-01-15T17:22:04Z",
    "timestamp": 1705339324000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.aaa4938"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2015,
        9,
        18
      ]
    ]
  },
  "references-count": 41,
  "journal-issue": {
    "issue": "6254",
    "published-print": {
      "date-parts": [
        [
          2015,
          9,
          18
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.aaa4938"
  ],
  "URL": "http://dx.doi.org/10.1126/science.aaa4938",
  "relation": {
    "has-review": [
      {
        "id-type": "doi",
        "id": "10.3410/f.725796916.793510091",
        "asserted-by": "object"
      }
    ]
  },
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        2015,
        9,
        18
      ]
    ]
  }
}