DOI: 10.1126/science.867052. Freeze-Fractured Purple Membrane Particles: Protein Content

Freeze-Fractured Purple Membrane Particles: Protein Content

10.1126/science.867052

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Optical diffraction and image reconstruction can be used to correlate the electron microscope image of the biological membrane with its electron density projection. Such correlation shows that a single purple membrane particle contains 9 to 12 protein molecules—63 to 84 transmembrane alpha helices—a complexity two to ten times greater than that previously suggested for membrane particles.

Bibliography

Fisher, K. A., & Stoeckenius, W. (1977). Freeze-Fractured Purple Membrane Particles: Protein Content. Science, 197(4298), 72â74.

Authors 2

Knute A. Fisher (first)
Walther Stoeckenius (additional)

References 27 Referenced 60

Abermann, R, Principles and Techniques of Electron Microscopy 2: 195 (1972). / Principles and Techniques of Electron Microscopy (1972)
BLAUROCK, A. E., NATURE-NEW BIOLOGY 233: 152 (1971). / NATURE-NEW BIOLOGY (1971)
Branton D. Membrane Structure (1972). (10.1007/978-3-7091-5600-1)
BRANTON, D, FRACTURE FACES OF FROZEN MEMBRANES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 55: 1048 (1966). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1966)
CHEN, Y.S., TEMPERATURE-DEPENDENT AND LIGHT-DEPENDENT STRUCTURAL-CHANGES IN RHODOPSIN-LIPID MEMBRANES, EXPERIMENTAL EYE RESEARCH 17: 517 (1973). (10.1016/0014-4835(73)90082-1) / EXPERIMENTAL EYE RESEARCH (1973)
Deamer, D. W., The Role of Membranes in Metabolic Regulation: 17 (1972). / The Role of Membranes in Metabolic Regulation (1972)
FISHER, K, METHODS IN ENZYMOLOGY 32: 35 (1974). (10.1016/0076-6879(74)32006-X) / METHODS IN ENZYMOLOGY (1974)
10.1126/science.1188378
GRANT, CWM, GLYCOPHORIN IN LIPID BILAYERS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 71: 4653 (1974). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1974)
HARRISON, P.M., STRUCTURE OF APOFERRITIN - EVIDENCE FOR CHEMICAL SUBUNITS FROM FINGERPRINTS OF TRYPTIC DIGESTS, JOURNAL OF MOLECULAR BIOLOGY 4: 239 (1962). (10.1016/S0022-2836(62)80002-3) / JOURNAL OF MOLECULAR BIOLOGY (1962)
Henderson, R., Annual Review of Biophysics and Bioengineering 6: 87 (1977). (10.1146/annurev.bb.06.060177.000511) / Annual Review of Biophysics and Bioengineering (1977)
HENDERSON, R, STRUCTURE OF PURPLE MEMBRANE FROM HALOBACTERIUM-HALOBIUM - ANALYSIS OF X-RAY-DIFFRACTION PATTERN, JOURNAL OF MOLECULAR BIOLOGY 93: 123 (1975). (10.1016/0022-2836(75)90123-0) / JOURNAL OF MOLECULAR BIOLOGY (1975)
HENDERSON, R, 3-DIMENSIONAL MODEL OF PURPLE MEMBRANE OBTAINED BY ELECTRON-MICROSCOPY, NATURE 257: 28 (1975). (10.1038/257028a0) / NATURE (1975)
HONG, K, PREPARATION AND PROPERTIES OF PHOSPHOLIPID BILAYERS CONTAINING RHODOPSIN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 69: 2617 (1972). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1972)
KLUG, A, OPTICAL FILTERING OF ELECTRON MICROGRAPHS - RECONSTRUCTION OF ONE-SIDED IMAGES, NATURE 212: 29 (1966). (10.1038/212029a0) / NATURE (1966)
LABAW, L, THE ELECTRON MICROSCOPY OF FERRITIN CRYSTALS, BIOCHIMICA ET BIOPHYSICA ACTA 25: 263 (1957). (10.1016/0006-3002(57)90467-5) / BIOCHIMICA ET BIOPHYSICA ACTA (1957)
MALAN, N.T., FUNCTIONAL AND STRUCTURAL ROLES OF SARCOPLASMIC-RETICULUM PROTEIN COMPONENTS, FEBS LETTERS 60: 122 (1975). (10.1016/0014-5793(75)80432-7) / FEBS LETTERS (1975)
OESTERHELT, D, METHODS IN ENZYMOLOGY 31: 667 (1974). (10.1016/0076-6879(74)31072-5) / METHODS IN ENZYMOLOGY (1974)
OESTERHELT, D, NATURE-NEW BIOLOGY 233: 149 (1971). / NATURE-NEW BIOLOGY (1971)
OESTERHELT, D, FUNCTIONS OF A NEW PHOTORECEPTOR MEMBRANE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 70: 2853 (1973). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1973)
PINTODASILVA, P, FREEZE-ETCH LOCALIZATION OF CONCANAVALIN-A RECEPTORS TO MEMBRANE INTERCALATED PARTICLES OF HUMAN ERYTHROCYTE GHOST MEMBRANES, BIOCHIMICA ET BIOPHYSICA ACTA 363: 311 (1974). (10.1016/0005-2736(74)90071-6) / BIOCHIMICA ET BIOPHYSICA ACTA (1974)
SEGREST, J.P., ASSOCIATION OF MEMBRANE-PENETRATING POLYPEPTIDE SEGMENT OF HUMAN ERYTHROCYTE MN-GLYCOPROTEIN WITH PHOSPHOLIPID BILAYERS .1. FORMATION OF FREEZE-ETCH INTRAMEMBRANOUS PARTICLES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 71: 3294 (1974). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1974)
10.1126/science.175.4023.720
STOECKENIUS, W, A MORPHOLOGICAL STUDY OF HALOBACTERIUM HALOBIUM AND ITS LYSIS IN MEDIA OF LOW SALT CONCENTRATION, JOURNAL OF CELL BIOLOGY 34: 365 (1967). (10.1083/jcb.34.1.365) / JOURNAL OF CELL BIOLOGY (1967)
UNWIN, PNT, MOLECULAR-STRUCTURE DETERMINATION BY ELECTRON-MICROSCOPY OF UNSTAINED CRYSTALLINE SPECIMENS, JOURNAL OF MOLECULAR BIOLOGY 94: 425 (1975). (10.1016/0022-2836(75)90212-0) / JOURNAL OF MOLECULAR BIOLOGY (1975)
YAMANAKA, N, PROTEASE DIGESTION OF MEMBRANES ULTRASTRUCTURAL AND BIOCHEMICAL EFFECTS, BIOCHIMICA ET BIOPHYSICA ACTA 426: 132 (1976). (10.1016/0005-2736(76)90435-1) / BIOCHIMICA ET BIOPHYSICA ACTA (1976)
YU, J, RECONSTITUTION OF INTRAMEMBRANE PARTICLES IN RECOMBINANTS OF ERYTHROCYTE PROTEIN BAND-3 AND LIPID - EFFECTS OF SPECTRIN-ACTIN ASSOCIATION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 73: 3891 (1976). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1976)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 12:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 15, 2024, 6:15 a.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 1:12 a.m.)
Issued	48 years, 1 month ago (July 1, 1977)
Published	48 years, 1 month ago (July 1, 1977)
Published Print	48 years, 1 month ago (July 1, 1977)

Funders 0

None

BibTeX

@article{Fisher_1977, title={Freeze-Fractured Purple Membrane Particles: Protein Content}, volume={197}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.867052}, DOI={10.1126/science.867052}, number={4298}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Fisher, Knute A. and Stoeckenius, Walther}, year={1977}, month=jul, pages={72–74} }

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