DOI: 10.1126/science.8475386. Regulation of TCR Signaling by CD45 Lacking Transmembrane and Extracellular Domains

Regulation of TCR Signaling by CD45 Lacking Transmembrane and Extracellular Domains

10.1126/science.8475386

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The CD45 protein is a transmembrane tyrosine phosphatase that is required for normal T cell receptor (TCR)-mediated signaling. A chimeric complementary DNA encoding the intracellular enzymatically active portion of murine CD45 preceded by a short amino-terminal sequence from p60 c- src was transfected into CD45- T cells. Expression of this chimeric protein corrected most of the TCR signaling abnormalities observed in the absence of CD45, including TCR-mediated enhancement of tyrosine kinase activity and Ca 2+ flux. Thus, the enzymatically active intracellular portion of CD45 is sufficient to allow TCR transmembrane signaling.

Bibliography

VolareviÄ, S., Niklinska, B. B., Burns, C. M., June, C. H., Weissman, A. M., & Ashwell, J. D. (1993). Regulation of TCR Signaling by CD45 Lacking Transmembrane and Extracellular Domains. Science, 260(5107), 541â544.

Authors 6

Siniša Volarević (first)
Barbara B. Niklinska (additional)
Christopher M. Burns (additional)
Carl H. June (additional)
Allan M. Weissman (additional)
Jonathan D. Ashwell (additional)

References 29 Referenced 100

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VOLAREVIC S unpublished data.

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:05 p.m.)
Deposited	1 year, 7 months ago (Jan. 15, 2024, 6:27 a.m.)
Indexed	5 months ago (March 30, 2025, 3:33 a.m.)
Issued	32 years, 4 months ago (April 23, 1993)
Published	32 years, 4 months ago (April 23, 1993)
Published Print	32 years, 4 months ago (April 23, 1993)

Funders 0

None

BibTeX

@article{Volarevi__1993, title={Regulation of TCR Signaling by CD45 Lacking Transmembrane and Extracellular Domains}, volume={260}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8475386}, DOI={10.1126/science.8475386}, number={5107}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Volarević, Siniša and Niklinska, Barbara B. and Burns, Christopher M. and June, Carl H. and Weissman, Allan M. and Ashwell, Jonathan D.}, year={1993}, month=apr, pages={541–544} }

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