Negative Regulation of G1 in Mammalian Cells: Inhibition of Cyclin E-Dependent Kinase by TGF-β
10.1126/science.8475385
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Transforming growth factor-β (TGF-β) is a naturally occurring growth inhibitory polypeptide that arrests the cell cycle in middle to late G1 phase. Cells treated with TGF-β contained normal amounts of cyclin E and cyclin-dependent protein kinase 2 (Cdk2) but failed to stably assemble cyclin E-Cdk2 complexes or accumulate cyclin E-associated kinase activity. Moreover, G1 phase extracts from TGF-β-treated cells did not support activation of endogenous cyclin-dependent protein kinases by exogenous cyclins. These effects of TGF-β, which correlated with the inhibition of retinoblastoma protein phosphorylation, suggest that mammalian G1 cyclin-dependent kinases, like their counterparts in yeast, are targets for negative regulators of the cell cycle.

Bibliography

Koff, A., Ohtsuki, M., Polyak, K., Roberts, J. M., & Massagué, J. (1993). Negative Regulation of G1 in Mammalian Cells: Inhibition of Cyclin E-Dependent Kinase by TGF-β. Science, 260(5107), 536–539.

Authors 5
  1. Andrew Koff (first)
  2. Masahiko Ohtsuki (additional)
  3. Kornelia Polyak (additional)
  4. James M. Roberts (additional)
  5. Joan Massagué (additional)
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Dates
Type When
Created 18 years, 10 months ago (Oct. 5, 2006, 7:05 p.m.)
Deposited 1 year, 7 months ago (Jan. 15, 2024, 6:28 a.m.)
Indexed 1 month, 4 weeks ago (July 1, 2025, 9:31 a.m.)
Issued 32 years, 4 months ago (April 23, 1993)
Published 32 years, 4 months ago (April 23, 1993)
Published Print 32 years, 4 months ago (April 23, 1993)
Funders 0

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@article{Koff_1993, title={Negative Regulation of G1 in Mammalian Cells: Inhibition of Cyclin E-Dependent Kinase by TGF-β}, volume={260}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8475385}, DOI={10.1126/science.8475385}, number={5107}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Koff, Andrew and Ohtsuki, Masahiko and Polyak, Kornelia and Roberts, James M. and Massagué, Joan}, year={1993}, month=apr, pages={536–539} }