DOI: 10.1126/science.8456316. Translocation of TCRα Chains into the Lumen of the Endoplasmic Reticulum and Their Degradation

Translocation of TCRα Chains into the Lumen of the Endoplasmic Reticulum and Their Degradation

10.1126/science.8456316

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

After synthesis, the α chain of the T cell antigen receptor (TCRα) can form a complex with other TCR chains and move to the cell surface, or TCRα can undergo degradation in the endoplasmic reticulum (ER) if it remains unassembled. The mechanism of translocation and degradation in the ER is unclear. It was found that the putative transmembrane region of TCRα (α tm ) was incompetent on its own to act as a transmembrane region. Molecules that contained α tm were translocated into the ER lumen and then underwent either rapid degradation or secretion, depending on the sequence of the cytoplasmic domain. A specific signal for ER degradation within α tm does not appear to be present.

Bibliography

Shin, J., Lee, S., & Strominger, J. L. (1993). Translocation of TCRÎ± Chains into the Lumen of the Endoplasmic Reticulum and Their Degradation. Science, 259(5103), 1901â1904.

Authors 3

Jaekyoon Shin (first)
Songjae Lee (additional)
Jack L. Strominger (additional)

References 23 Referenced 52

ALARCON, B, ASSEMBLY OF THE HUMAN T-CELL RECEPTOR-CD3 COMPLEX TAKES PLACE IN THE ENDOPLASMIC-RETICULUM AND INVOLVES INTERMEDIARY COMPLEXES BETWEEN THE CD3-GAMMA-DELTA-EPSILON CORE AND SINGLE T-CELL RECEPTOR ALPHA-CHAINS OR BETA-CHAINS, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 2953 (1988). (10.1016/S0021-9258(18)69161-6) / JOURNAL OF BIOLOGICAL CHEMISTRY (1988)
BANIYASH, M, DISULFIDE LINKAGE OF THE ZETA-CHAIN AND ETA-CHAIN OF THE T-CELL RECEPTOR POSSIBLE IDENTIFICATION OF 2 STRUCTURAL CLASSES OF RECEPTORS, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 9874 (1988). (10.1016/S0021-9258(19)81598-3) / JOURNAL OF BIOLOGICAL CHEMISTRY (1988)
BONIFACINO, J.S., COLOCALIZED TRANSMEMBRANE DETERMINANTS FOR ER DEGRADATION AND SUBUNIT ASSEMBLY EXPLAIN THE INTRACELLULAR FATE OF TCR CHAINS, CELL 63: 503 (1990). (10.1016/0092-8674(90)90447-M) / CELL (1990)
BONIFACINO, J.S., ROLE OF POTENTIALLY CHARGED TRANSMEMBRANE RESIDUES IN TARGETING PROTEINS FOR RETENTION AND DEGRADATION WITHIN THE ENDOPLASMIC-RETICULUM, EMBO JOURNAL 10: 2783 (1991). (10.1002/j.1460-2075.1991.tb07827.x) / EMBO JOURNAL (1991)
10.1126/science.2294595
BORDIER, C, PHASE-SEPARATION OF INTEGRAL MEMBRANE-PROTEINS IN TRITON X-114 SOLUTION, JOURNAL OF BIOLOGICAL CHEMISTRY 256: 1604 (1981). (10.1016/S0021-9258(19)69848-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1981)
CHEN, C, HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA, MOLECULAR AND CELLULAR BIOLOGY 7: 2745 (1987). / MOLECULAR AND CELLULAR BIOLOGY (1987)
CLEVERS, H, THE T-CELL RECEPTOR/CD3 COMPLEX - A DYNAMIC PROTEIN ENSEMBLE, ANNUAL REVIEW OF IMMUNOLOGY 6: 629 (1988). (10.1146/annurev.iy.06.040188.003213) / ANNUAL REVIEW OF IMMUNOLOGY (1988)
Creighton, T. E., Proteins: 142 (1984). / Proteins (1984)
10.1016/0022-2836(84)90309-7
GOVERMAN, J, CHIMERIC IMMUNOGLOBULIN T-CELL RECEPTOR PROTEINS FORM FUNCTIONAL RECEPTORS - IMPLICATIONS FOR T-CELL RECEPTOR COMPLEX-FORMATION AND ACTIVATION, CELL 60: 929 (1990). (10.1016/0092-8674(90)90341-B) / CELL (1990)
KUCHROO, V.K., T-CELL RECEPTOR ALPHA-CHAIN PLAYS A CRITICAL ROLE IN ANTIGEN-SPECIFIC SUPPRESSOR-CELL FUNCTION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 8700 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
LEIDEN, J.M., THE COMPLETE PRIMARY STRUCTURE OF THE T-CELL RECEPTOR GENES FROM AN ALLOREACTIVE CYTOTOXIC HUMAN LYMPHOCYTE-T CLONE, IMMUNOGENETICS 24: 17 (1986). (10.1007/BF00372293) / IMMUNOGENETICS (1986)
LIPPINCOTTSCHWA.J, DEGRADATION FROM THE ENDOPLASMIC-RETICULUM - DISPOSING OF NEWLY SYNTHESIZED PROTEINS, CELL 54: 209 (1988). (10.1016/0092-8674(88)90553-3) / CELL (1988)
LIPPINCOTTSCHWA.J, MICROTUBULE-DEPENDENT RETROGRADE TRANSPORT OF PROTEINS INTO THE ER IN THE PRESENCE OF BREFELDIN-A SUGGESTS AN ER RECYCLING PATHWAY, CELL 60: 821 (1990). (10.1016/0092-8674(90)90096-W) / CELL (1990)
10.1126/science.2142801
SHIN, J, STRUCTURAL FEATURES OF THE CYTOPLASMIC REGION OF CD4 REQUIRED FOR INTERNALIZATION, EMBO JOURNAL 9: 425 (1990). (10.1002/j.1460-2075.1990.tb08127.x) / EMBO JOURNAL (1990)
SHIN, J, SIGNALS FOR RETENTION OF TRANSMEMBRANE PROTEINS IN THE ENDOPLASMIC-RETICULUM STUDIED WITH CD4 TRUNCATION MUTANTS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 1918 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
SHIN J unpublished data.
SHIN, J.Y., PHOSPHORYLATION-DEPENDENT DOWN-MODULATION OF CD4 REQUIRES A SPECIFIC STRUCTURE WITHIN THE CYTOPLASMIC DOMAIN OF CD4, JOURNAL OF BIOLOGICAL CHEMISTRY 266: 10658 (1991). (10.1016/S0021-9258(18)99274-4) / JOURNAL OF BIOLOGICAL CHEMISTRY (1991)
10.1016/0092-8674(90)90092-S
SITIA, R, REGULATION OF MEMBRANE IGM EXPRESSION IN SECRETORY B-CELLS - TRANSLATIONAL AND POST-TRANSLATIONAL EVENTS, EMBO JOURNAL 6: 3969 (1987). (10.1002/j.1460-2075.1987.tb02739.x) / EMBO JOURNAL (1987)
WILEMAN, T, THE GAMMA SUBUNITS AND EPSILON SUBUNITS OF THE CD3 COMPLEX INHIBIT PRE-GOLGI DEGRADATION OF NEWLY SYNTHESIZED T-CELL ANTIGEN RECEPTORS, JOURNAL OF CELL BIOLOGY 110: 973 (1990). (10.1083/jcb.110.4.973) / JOURNAL OF CELL BIOLOGY (1990)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:05 p.m.)
Deposited	1 year, 7 months ago (Jan. 15, 2024, 6:29 a.m.)
Indexed	3 months, 1 week ago (May 16, 2025, 6:04 a.m.)
Issued	32 years, 4 months ago (March 26, 1993)
Published	32 years, 4 months ago (March 26, 1993)
Published Print	32 years, 4 months ago (March 26, 1993)

Funders 0

None

BibTeX

@article{Shin_1993, title={Translocation of TCRα Chains into the Lumen of the Endoplasmic Reticulum and Their Degradation}, volume={259}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8456316}, DOI={10.1126/science.8456316}, number={5103}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Shin, Jaekyoon and Lee, Songjae and Strominger, Jack L.}, year={1993}, month=mar, pages={1901–1904} }

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