DOI: 10.1126/science.8451644. Requirement for a GTPase-Activating Protein in Vesicle Budding from the Endoplasmic Reticulum

Requirement for a GTPase-Activating Protein in Vesicle Budding from the Endoplasmic Reticulum

10.1126/science.8451644

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The binding and hydrolysis of guanosine triphosphate (GTP) by the small GTP-binding protein Sar1p is required to form transport vesicles from the endoplasmic reticulum (ER) in Saccharomyces cerevisiae . Experiments revealed that an interaction between Sar1p and the Sec23p subunit of an oligomeric protein is also required for vesicle budding. The isolated Sec23p subunit and the oligomeric complex stimulated guanosine triphosphatase (GTPase) activity of Sar1p 10- to 15-fold but did not activate two other small GTP-binding proteins involved in vesicle traffic (Ypt1p and ARF). Activation of GTPase was inhibited by an antibody to Sec23p but not by an antibody that inhibits the budding activity of the other subunit of the Sec23p complex. Also, activation was thermolabile in pure samples of Sec23p that were isolated from two independent sec23 mutant strains. It appears that Sec23p represents a new class of GTPase-activating protein because its sequence shows no similarity to any known member of this family.

Bibliography

Yoshihisa, T., Barlowe, C., & Schekman, R. (1993). Requirement for a GTPase-Activating Protein in Vesicle Budding from the Endoplasmic Reticulum. Science, 259(5100), 1466â1468.

Authors 3

Tohru Yoshihisa (first)
Charles Barlowe (additional)
Randy Schekman (additional)

References 27 Referenced 291

BARLOWE, C, PURIFICATION AND CHARACTERIZATION OF SAR1P, A SMALL GTP-BINDING PROTEIN REQUIRED FOR TRANSPORT VESICLE FORMATION FROM THE ENDOPLASMIC-RETICULUM, JOURNAL OF BIOLOGICAL CHEMISTRY 268: 873 (1993). (10.1016/S0021-9258(18)54015-1) / JOURNAL OF BIOLOGICAL CHEMISTRY (1993)
BOBAK, D.A., MECHANISM OF ACTIVATION OF CHOLERA-TOXIN BY ADP-RIBOSYLATION FACTOR (ARF) - BOTH LOW-AFFINITY AND HIGH-AFFINITY INTERACTIONS OF ARF WITH GUANINE-NUCLEOTIDES PROMOTE TOXIN ACTIVATION, BIOCHEMISTRY 29: 855 (1990). (10.1021/bi00456a600) / BIOCHEMISTRY (1990)
BOLLAG, G, REGULATORS AND EFFECTORS OF RAS PROTEINS, ANNUAL REVIEW OF CELL BIOLOGY 7: 601 (1991). (10.1146/annurev.cb.07.110191.003125) / ANNUAL REVIEW OF CELL BIOLOGY (1991)
10.1038/349117a0
10.1126/science.2188363
DENFERT, C, SEC12P-DEPENDENT MEMBRANE-BINDING OF THE SMALL GTP-BINDING PROTEIN SAR1P PROMOTES FORMATION OF TRANSPORT VESICLES FROM THE ER, JOURNAL OF CELL BIOLOGY 114: 663 (1991). (10.1083/jcb.114.4.663) / JOURNAL OF CELL BIOLOGY (1991)
HARIHARAN, I.K., CDNA SEQUENCE FOR HUMAN-BCR, THE GENE THAT TRANSLOCATES TO THE ABL-ONCOGENE IN CHRONIC MYELOID-LEUKEMIA, EMBO JOURNAL 6: 115 (1987). (10.1002/j.1460-2075.1987.tb04727.x) / EMBO JOURNAL (1987)
HICKE, L, YEAST SEC23P ACTS IN THE CYTOPLASM TO PROMOTE PROTEIN-TRANSPORT FROM THE ENDOPLASMIC-RETICULUM TO THE GOLGI-COMPLEX INVIVO AND INVITRO, EMBO JOURNAL 8: 1677 (1989). (10.1002/j.1460-2075.1989.tb03559.x) / EMBO JOURNAL (1989)
HICKE, L, SEC23P AND A NOVEL 105-KDA PROTEIN FUNCTION AS A MULTIMERIC COMPLEX TO PROMOTE VESICLE BUDDING AND PROTEIN-TRANSPORT FROM THE ENDOPLASMIC-RETICULUM, MOLECULAR BIOLOGY OF THE CELL 3: 667 (1992). (10.1091/mbc.3.6.667) / MOLECULAR BIOLOGY OF THE CELL (1992)
KAISER, C.A., DISTINCT SETS OF SEC GENES GOVERN TRANSPORT VESICLE FORMATION AND FUSION EARLY IN THE SECRETORY PATHWAY, CELL 61: 723 (1990). (10.1016/0092-8674(90)90483-U) / CELL (1990)
NAKANO, A, A NOVEL GTP-BINDING PROTEIN, SAR1P, IS INVOLVED IN TRANSPORT FROM THE ENDOPLASMIC-RETICULUM TO THE GOLGI-APPARATUS, JOURNAL OF CELL BIOLOGY 109: 2677 (1989). (10.1083/jcb.109.6.2677) / JOURNAL OF CELL BIOLOGY (1989)
10.1016/S0021-9258(18)33775-X
OKA, T, RECONSTITUTION OF GTP-BINDING SAR1 PROTEIN FUNCTION IN ER TO GOLGI TRANSPORT, JOURNAL OF CELL BIOLOGY 114: 671 (1991). (10.1083/jcb.114.4.671) / JOURNAL OF CELL BIOLOGY (1991)
ORCI, L, MAMMALIAN SEC23P HOMOLOG IS RESTRICTED TO THE ENDOPLASMIC-RETICULUM TRANSITIONAL CYTOPLASM, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 8611 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
ORRWEAVER, T.L., GENETIC APPLICATIONS OF YEAST TRANSFORMATION WITH LINEAR AND GAPPED PLASMIDS, METHODS IN ENZYMOLOGY 101: 228 (1983). (10.1016/0076-6879(83)01017-4) / METHODS IN ENZYMOLOGY (1983)
PFEFFER, S.R., TRENDS CELL BIOL 2: 41 (1992). (10.1016/0962-8924(92)90161-F) / TRENDS CELL BIOL (1992)
PRYER, N.K., VESICLE-MEDIATED PROTEIN SORTING, ANNUAL REVIEW OF BIOCHEMISTRY 61: 471 (1992). (10.1146/annurev.bi.61.070192.002351) / ANNUAL REVIEW OF BIOCHEMISTRY (1992)
REXACH, M.F., DISTINCT BIOCHEMICAL REQUIREMENTS FOR THE BUDDING, TARGETING, AND FUSION OF ER-DERIVED TRANSPORT VESICLES, JOURNAL OF CELL BIOLOGY 114: 219 (1991). (10.1083/jcb.114.2.219) / JOURNAL OF CELL BIOLOGY (1991)
10.1038/355409a0
RUBINFELD, B, MOLECULAR-CLONING OF A GTPASE ACTIVATING PROTEIN-SPECIFIC FOR THE KREV-1 PROTEIN P21RAP1, CELL 65: 1033 (1991). (10.1016/0092-8674(91)90555-D) / CELL (1991)
10.1126/science.1904626
10.1016/0092-8674(90)90094-U
10.1038/335090a0
WAGNER, P, BIOCHEMICAL-PROPERTIES OF THE RAS-RELATED YPT PROTEIN IN YEAST - A MUTATIONAL ANALYSIS, EMBO JOURNAL 6: 2373 (1987). (10.1002/j.1460-2075.1987.tb02514.x) / EMBO JOURNAL (1987)
WEISS, O, NUCLEOTIDE BINDING AND COFACTOR ACTIVITIES OF PURIFIED BOVINE BRAIN AND BACTERIALLY EXPRESSED ADP-RIBOSYLATION FACTOR, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 21066 (1989). (10.1016/S0021-9258(19)30046-8) / JOURNAL OF BIOLOGICAL CHEMISTRY (1989)
YOSHIHISA T unpublished data.
10.1016/0092-8674(90)90024-9

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:05 p.m.)
Deposited	1 year, 7 months ago (Jan. 15, 2024, 6:35 a.m.)
Indexed	2 weeks, 1 day ago (Aug. 7, 2025, 4:41 p.m.)
Issued	32 years, 5 months ago (March 5, 1993)
Published	32 years, 5 months ago (March 5, 1993)
Published Print	32 years, 5 months ago (March 5, 1993)

Funders 0

None

BibTeX

@article{Yoshihisa_1993, title={Requirement for a GTPase-Activating Protein in Vesicle Budding from the Endoplasmic Reticulum}, volume={259}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8451644}, DOI={10.1126/science.8451644}, number={5100}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Yoshihisa, Tohru and Barlowe, Charles and Schekman, Randy}, year={1993}, month=mar, pages={1466–1468} }

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