DOI: 10.1126/science.8346442. Reconstitution of T Cell Receptor ζ-Mediated Calcium Mobilization in Nonlymphoid Cells

Reconstitution of T Cell Receptor ζ-Mediated Calcium Mobilization in Nonlymphoid Cells

10.1126/science.8346442

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

T cell antigen receptor (TCR) activation involves interactions between receptor subunits and nonreceptor protein tyrosine kinases (PTKs). Early steps in signaling through the ζ chain of the TCR were examined in transfected COS-1 cells. Coexpression of the PTK p59 fynT , but not p56 lck , with ζ or with a homodimeric TCRβ-ζ fusion protein produced tyrosine phosphorylation of both ζ and phospholipase C (PLC)-γ1, as well as calcium ion mobilization in response to receptor cross-linking. CD45 coexpression enhanced these effects. No requirement for the PTKZAP-70 was observed. Thus, p59 fynT may link ζ directly to the PLC-γ1 activation pathway.

Bibliography

Hall, C. G., Sancho, J., & Terhorst, C. (1993). Reconstitution of T Cell Receptor Î¶-Mediated Calcium Mobilization in Nonlymphoid Cells. Science, 261(5123), 915â918.

Authors 3

Craig G. Hall (first)
Jaime Sancho (additional)
Cox Terhorst (additional)

References 45 Referenced 55

ABRAHAM, K.M., THYMIC TUMORIGENESIS INDUCED BY OVEREXPRESSION OF P56LCK, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 3977 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
APPLEBY, M.W., DEFECTIVE T-CELL RECEPTOR SIGNALING IN MICE LACKING THE THYMIC ISOFORM OF P59(FYN), CELL 70: 751 (1992). (10.1016/0092-8674(92)90309-Z) / CELL (1992)
ARUFFO, A, MOLECULAR-CLONING OF A CD28 CDNA BY A HIGH-EFFICIENCY COS CELL EXPRESSION SYSTEM, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84: 8573 (1987). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1987)
BANIYASH, M, THE T-CELL ANTIGEN RECEPTOR ZETA-CHAIN IS TYROSINE PHOSPHORYLATED UPON ACTIVATION, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 18225 (1988). (10.1016/S0021-9258(19)81349-2) / JOURNAL OF BIOLOGICAL CHEMISTRY (1988)
BARBER, E.K., THE CD4 AND CD8 ANTIGENS ARE COUPLED TO A PROTEIN-TYROSINE KINASE (P56LCK) THAT PHOSPHORYLATES THE CD3 COMPLEX, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 3277 (1989). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1989)
10.1038/361315a0
CHAN, A.C., ZAP-70 - A 70 KD PROTEIN-TYROSINE KINASE THAT ASSOCIATES WITH THE TCR ZETA-CHAIN, CELL 71: 649 (1992). (10.1016/0092-8674(92)90598-7) / CELL (1992)
CHAN, A.C., THE ZETA-CHAIN IS ASSOCIATED WITH A TYROSINE KINASE AND UPON T-CELL ANTIGEN RECEPTOR STIMULATION ASSOCIATES WITH ZAP-70, A 70-KDA TYROSINE PHOSPHOPROTEIN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 9166 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
COOKE, M.P., REGULATION OF T-CELL RECEPTOR SIGNALING BY A SRC FAMILY PROTEIN-TYROSINE KINASE (P59FYN), CELL 65: 281 (1991). (10.1016/0092-8674(91)90162-R) / CELL (1991)
ENGEL, I, HIGH-EFFICIENCY EXPRESSION AND SOLUBILIZATION OF FUNCTIONAL T-CELL ANTIGEN RECEPTOR HETERODIMERS, SCIENCE 256: 1318 (1992). (10.1126/science.1598575) / SCIENCE (1992)
GAUEN, LKT, P59FYN TYROSINE KINASE ASSOCIATES WITH MULTIPLE T-CELL RECEPTOR SUBUNITS THROUGH ITS UNIQUE AMINO-TERMINAL DOMAIN, MOLECULAR AND CELLULAR BIOLOGY 12: 5438 (1992). / MOLECULAR AND CELLULAR BIOLOGY (1992)
HALL C.G. unpublished data.
IRVING, B.A., THE CYTOPLASMIC DOMAIN OF THE T-CELL RECEPTOR ZETA-CHAIN IS SUFFICIENT TO COUPLE TO RECEPTOR-ASSOCIATED SIGNAL TRANSDUCTION PATHWAYS, CELL 64: 891 (1991). (10.1016/0092-8674(91)90314-O) / CELL (1991)
IRVING, B.A., FUNCTIONAL-CHARACTERIZATION OF A SIGNAL TRANSDUCING MOTIF PRESENT IN THE T-CELL ANTIGEN RECEPTOR ZETA-CHAIN, JOURNAL OF EXPERIMENTAL MEDICINE 177: 1093 (1993). (10.1084/jem.177.4.1093) / JOURNAL OF EXPERIMENTAL MEDICINE (1993)
JUNE, C.H., INCREASES IN TYROSINE PHOSPHORYLATION ARE DETECTABLE BEFORE PHOSPHOLIPASE-C ACTIVATION AFTER T-CELL RECEPTOR STIMULATION, JOURNAL OF IMMUNOLOGY 144: 1591 (1990). (10.4049/jimmunol.144.5.1591) / JOURNAL OF IMMUNOLOGY (1990)
JUNE, C.H., INHIBITION OF TYROSINE PHOSPHORYLATION PREVENTS T-CELL RECEPTOR-MEDIATED SIGNAL TRANSDUCTION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 87: 7722 (1990). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1990)
KORETZKY, G.A., TYROSINE PHOSPHATASE CD45 IS ESSENTIAL FOR COUPLING T-CELL ANTIGEN RECEPTOR TO THE PHOSPHATIDYL INOSITOL PATHWAY, NATURE 346: 66 (1990). (10.1038/346066a0) / NATURE (1990)
KORETZKY, G.A., TYROSINE PHOSPHATASE CD45 IS REQUIRED FOR T-CELL ANTIGEN RECEPTOR AND CD2-MEDIATED ACTIVATION OF A PROTEIN TYROSINE KINASE AND INTERLEUKIN-2 PRODUCTION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 2037 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
KOYASU, S, PHOSPHORYLATION OF MULTIPLE CD3-ZETA TYROSINE RESIDUES LEADS TO FORMATION OF PP21 INVITRO AND INVIVO - STRUCTURAL-CHANGES UPON T-CELL RECEPTOR STIMULATION, JOURNAL OF BIOLOGICAL CHEMISTRY 267: 3375 (1992). (10.1016/S0021-9258(19)50741-4) / JOURNAL OF BIOLOGICAL CHEMISTRY (1992)
LEDBETTER, J.A., SEMIN IMMUNOL 2: 99 (1990). / SEMIN IMMUNOL (1990)
10.1126/science.1532456
MASLINSKI W unpublished data.
10.1126/science.2138816
PERLMUTTER, R.M., STRUCTURE AND EXPRESSION OF ICK - TRANSCRIPTS IN HUMAN LYMPHOID-CELLS, JOURNAL OF CELLULAR BIOCHEMISTRY 38: 117 (1988). (10.1002/jcb.240380206) / JOURNAL OF CELLULAR BIOCHEMISTRY (1988)
PINGEL, J.T., EVIDENCE THAT THE LEUKOCYTE-COMMON ANTIGEN IS REQUIRED FOR ANTIGEN-INDUCED LYMPHOCYTE-T PROLIFERATION, CELL 58: 1055 (1989). (10.1016/0092-8674(89)90504-7) / CELL (1989)
QIAN, D.P., MULTIPLE COMPONENTS OF THE T-CELL ANTIGEN RECEPTOR COMPLEX BECOME TYROSINE-PHOSPHORYLATED UPON ACTIVATION, JOURNAL OF BIOLOGICAL CHEMISTRY 268: 4488 (1993). (10.1016/S0021-9258(18)53635-8) / JOURNAL OF BIOLOGICAL CHEMISTRY (1993)
10.1038/338383b0
ROMEO, C, CELLULAR-IMMUNITY TO HIV ACTIVATED BY CD4 FUSED TO T-CELL OR FC RECEPTOR POLYPEPTIDES, CELL 64: 1037 (1991). (10.1016/0092-8674(91)90327-U) / CELL (1991)
ROMEO, C, SEQUENCE REQUIREMENTS FOR INDUCTION OF CYTOLYSIS BY THE T-CELL ANTIGEN/FC RECEPTOR ZETA-CHAIN, CELL 68: 889 (1992). (10.1016/0092-8674(92)90032-8) / CELL (1992)
SAMELSON, L.E., ANTIGEN ACTIVATION OF MURINE T-CELLS INDUCES TYROSINE PHOSPHORYLATION OF A POLYPEPTIDE ASSOCIATED WITH THE T-CELL ANTIGEN RECEPTOR, CELL 46: 1083 (1986). (10.1016/0092-8674(86)90708-7) / CELL (1986)
SAMELSON, L.E., TYROSINE KINASES AND TYROSINE-BASED ACTIVATION MOTIFS - CURRENT RESEARCH ON ACTIVATION VIA THE T-CELL ANTIGEN RECEPTOR, JOURNAL OF BIOLOGICAL CHEMISTRY 267: 24913 (1992). (10.1016/S0021-9258(19)73981-7) / JOURNAL OF BIOLOGICAL CHEMISTRY (1992)
SAMELSON, L.E., ASSOCIATION OF THE FYN PROTEIN-TYROSINE KINASE WITH THE T-CELL ANTIGEN RECEPTOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 87: 4358 (1990). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1990)
SANCHO, J, THE T-CELL RECEPTOR-ASSOCIATED CD3-EPSILON PROTEIN IS PHOSPHORYLATED UPON T-CELL ACTIVATION IN THE 2 TYROSINE RESIDUES OF A CONSERVED SIGNAL-TRANSDUCTION MOTIF, EUROPEAN JOURNAL OF IMMUNOLOGY 23: 1636 (1993). (10.1002/eji.1830230736) / EUROPEAN JOURNAL OF IMMUNOLOGY (1993)
SANCHO, J, CD3-ZETA SURFACE EXPRESSION IS REQUIRED FOR CD4-P56LCK-MEDIATED UP-REGULATION OF T-CELL ANTIGEN RECEPTOR-CD3 SIGNALING IN T-CELLS, JOURNAL OF BIOLOGICAL CHEMISTRY 267: 7871 (1992). (10.1016/S0021-9258(18)42594-X) / JOURNAL OF BIOLOGICAL CHEMISTRY (1992)
SEFTON, B.M., THE ROLE OF TYROSINE PROTEIN-PHOSPHORYLATION IN LYMPHOCYTE-ACTIVATION, ANNUAL REVIEW OF CELL BIOLOGY 7: 257 (1991). (10.1146/annurev.cb.07.110191.001353) / ANNUAL REVIEW OF CELL BIOLOGY (1991)
SEMBA, K, YES-RELATED PROTOONCOGENE, SYN, BELONGS TO THE PROTEIN-TYROSINE KINASE FAMILY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 5459 (1986). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1986)
STEIN, P.L., PP59(FYN) MUTANT MICE DISPLAY DIFFERENTIAL SIGNALING IN THYMOCYTES AND PERIPHERAL T-CELLS, CELL 70: 741 (1992). (10.1016/0092-8674(92)90308-Y) / CELL (1992)
STRAUS, D.B., GENETIC-EVIDENCE FOR THE INVOLVEMENT OF THE ICK TYROSINE KINASE IN SIGNAL TRANSDUCTION THROUGH THE T-CELL ANTIGEN RECEPTOR, CELL 70: 585 (1992). (10.1016/0092-8674(92)90428-F) / CELL (1992)
10.1016/0092-8674(90)90801-K
WEBER, J.R., ASSOCIATION OF THE TYROSINE KINASE LCK WITH PHOSPHOLIPASE C-GAMMA-1 AFTER STIMULATION OF THE T-CELL ANTIGEN RECEPTOR, JOURNAL OF EXPERIMENTAL MEDICINE 176: 373 (1992). (10.1084/jem.176.2.373) / JOURNAL OF EXPERIMENTAL MEDICINE (1992)
WEGENER, AMK, THE T-CELL RECEPTOR/CD3 COMPLEX IS COMPOSED OF AT LEAST 2 AUTONOMOUS TRANSDUCTION MODULES, CELL 68: 83 (1992). (10.1016/0092-8674(92)90208-T) / CELL (1992)
WEISS, A, FUNCTIONAL ACTIVATION OF THE T-CELL ANTIGEN RECEPTOR INDUCES TYROSINE PHOSPHORYLATION OF PHOSPHOLIPASE C-GAMMA-1, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 5484 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
10.1126/science.3278377
WILEMAN, T, THE TRANSMEMBRANE ANCHOR OF THE T-CELL ANTIGEN RECEPTOR BETA-CHAIN CONTAINS A STRUCTURAL DETERMINANT OF PRE-GOLGI PROTEOLYSIS, CELL REGULATION 1: 907 (1990). (10.1091/mbc.1.12.907) / CELL REGULATION (1990)
YANAGI, Y, A HUMAN T-CELL-SPECIFIC CDNA CLONE ENCODES A PROTEIN HAVING EXTENSIVE HOMOLOGY TO IMMUNOGLOBULIN-CHAINS, NATURE 308: 145 (1984). (10.1038/308145a0) / NATURE (1984)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:05 p.m.)
Deposited	1 year, 7 months ago (Jan. 15, 2024, 6:14 a.m.)
Indexed	9 months ago (Nov. 19, 2024, 11:02 a.m.)
Issued	32 years ago (Aug. 13, 1993)
Published	32 years ago (Aug. 13, 1993)
Published Print	32 years ago (Aug. 13, 1993)

Funders 0

None

BibTeX

@article{Hall_1993, title={Reconstitution of T Cell Receptor ζ-Mediated Calcium Mobilization in Nonlymphoid Cells}, volume={261}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8346442}, DOI={10.1126/science.8346442}, number={5123}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Hall, Craig G. and Sancho, Jaime and Terhorst, Cox}, year={1993}, month=aug, pages={915–918} }

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