DOI: 10.1126/science.8272869. Mitotic Repression of RNA Polymerase III Transcription in Vitro Mediated by Phosphorylation of a TFIIIB Component

Mitotic Repression of RNA Polymerase III Transcription in Vitro Mediated by Phosphorylation of a TFIIIB Component

10.1126/science.8272869

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Interphase cytosol extracts prepared from Xenopus laevis eggs are active in RNA polymerase III (Pol III) transcription. Addition of recombinant B1 cyclin to these extracts activates mitotic protein kinases that repress transcription. Affinity-purified p34 cdc2 -cyclin B kinase (mitosis-promoting factor) is sufficient to effect this repression in a simplified Pol III transcription system. This mitotic repression involves the direct phosphorylation of a component of the Pol III transcription initiation factor TFIIIB, which consists of the TATA box-binding protein (TBP) and associated Pol III-specific factors. The transcriptional activity of the TFIIIB-TBP fraction can be modulated in vitro by phosphorylation with mitotic kinases and by dephosphorylation with immobilized alkaline phosphatase.

Bibliography

Gottesfeld, J. M., Wolf, V. J., Dang, T., Forbes, D. J., & Hartl, P. (1994). Mitotic Repression of RNA Polymerase III Transcription in Vitro Mediated by Phosphorylation of a TFIIIB Component. Science, 263(5143), 81â84.

Authors 5

Joel M. Gottesfeld (first)
Veronica J. Wolf (additional)
Tam Dang (additional)
Douglass J. Forbes (additional)
Philippe Hartl (additional)

References 45 Referenced 90

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited	1 year, 7 months ago (Jan. 15, 2024, 5:31 a.m.)
Indexed	3 months, 2 weeks ago (May 20, 2025, 9:16 a.m.)
Issued	31 years, 7 months ago (Jan. 7, 1994)
Published	31 years, 7 months ago (Jan. 7, 1994)
Published Print	31 years, 7 months ago (Jan. 7, 1994)

Funders 0

None

BibTeX

@article{Gottesfeld_1994, title={Mitotic Repression of RNA Polymerase III Transcription in Vitro Mediated by Phosphorylation of a TFIIIB Component}, volume={263}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8272869}, DOI={10.1126/science.8272869}, number={5143}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Gottesfeld, Joel M. and Wolf, Veronica J. and Dang, Tam and Forbes, Douglass J. and Hartl, Philippe}, year={1994}, month=jan, pages={81–84} }

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