DOI: 10.1126/science.8248803. Binding to DNA and the Retinoblastoma Gene Product Promoted by Complex Formation of Different E2F Family Members

Binding to DNA and the Retinoblastoma Gene Product Promoted by Complex Formation of Different E2F Family Members

10.1126/science.8248803

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The E2F family of transcription factors functions in the control of the mammalian cell cycle. Here it is shown that two family members, E2F-1 and DP-1, form specific heterodimers in vivo, a process that enhances DNA binding, transactivation, and the binding of the retinoblastoma gene product. These results suggest that heterodimerization regulates E2F function and contributes to cell cycle control.

Bibliography

Krek, W., Livingston, D. M., & Shirodkar, S. (1993). Binding to DNA and the Retinoblastoma Gene Product Promoted by Complex Formation of Different E2F Family Members. Science, 262(5139), 1557â1560.

Authors 3

Wilhelm Krek (first)
David M. Livingston (additional)
Suman Shirodkar (additional)

References 27 Referenced 199

Ausubel F. M. Current Protocols in Molecular Biology 1 (1990).
10.1016/0092-8674(89)90508-4
CHEN, C, HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA, MOLECULAR AND CELLULAR BIOLOGY 7: 2745 (1987). / MOLECULAR AND CELLULAR BIOLOGY (1987)
10.1016/0092-8674(89)90517-5
10.1016/0092-8674(88)90559-4
10.1016/0092-8674(89)90507-2
10.1073/pnas.89.5.1795
FLEMINGTON, E.K., E2F-1-MEDIATED TRANSACTIVATION IS INHIBITED BY COMPLEX-FORMATION WITH THE RETINOBLASTOMA SUSCEPTIBILITY GENE-PRODUCT, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 90: 6914 (1993). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1993)
GINSBERG D unpublished data.
GIRLING, R, A NEW COMPONENT OF THE TRANSCRIPTION FACTOR-DRTF1/E2F, NATURE 362: 83 (1993). (10.1038/362083a0) / NATURE (1993)
HELIN, K, A CDNA-ENCODING A PRB-BINDING PROTEIN WITH PROPERTIES OF THE TRANSCRIPTION FACTOR E2F, CELL 70: 337 (1992). (10.1016/0092-8674(92)90107-N) / CELL (1992)
HELIN, K, INHIBITION OF E2F-1 TRANSACTIVATION BY DIRECT BINDING OF THE RETINOBLASTOMA PROTEIN, MOLECULAR AND CELLULAR BIOLOGY 13: 6501 (1993). / MOLECULAR AND CELLULAR BIOLOGY (1993)
HIEBERT, S.W., REGIONS OF THE RETINOBLASTOMA GENE-PRODUCT REQUIRED FOR ITS INTERACTION WITH THE E2F TRANSCRIPTION FACTOR ARE NECESSARY OF E2 PROMOTER REPRESSION AND PRB-MEDIATED GROWTH SUPPRESSION, MOLECULAR AND CELLULAR BIOLOGY 13: 3384 (1993). / MOLECULAR AND CELLULAR BIOLOGY (1993)
HU, Q.J., ANTIBODIES SPECIFIC FOR THE HUMAN RETINOBLASTOMA PROTEIN IDENTIFY A FAMILY OF RELATED POLYPEPTIDES, MOLECULAR AND CELLULAR BIOLOGY 11: 5792 (1991). / MOLECULAR AND CELLULAR BIOLOGY (1991)
HUBER, H.E., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 90: 35 (1993). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1993)
KAELIN, W.G., EXPRESSION CLONING OF A CDNA-ENCODING A RETINOBLASTOMA-BINDING PROTEIN WITH E2F-LIKE PROPERTIES, CELL 70: 351 (1992). (10.1016/0092-8674(92)90108-O) / CELL (1992)
KREK, W, CASEIN KINASE-II IS A PREDOMINANTLY NUCLEAR ENZYME, JOURNAL OF CELL BIOLOGY 116: 43 (1992). (10.1083/jcb.116.1.43) / JOURNAL OF CELL BIOLOGY (1992)
KREK W unpublished data.
Lees, J., Molecular and Cellular Biology 13: 7813 (1993). / Molecular and Cellular Biology (1993)
LUDLOW, J.W., THE RETINOBLASTOMA SUSCEPTIBILITY GENE-PRODUCT UNDERGOES CELL CYCLE-DEPENDENT DEPHOSPHORYLATION AND BINDING TO AND RELEASE FROM SV40 LARGE-T, CELL 60: 387 (1990). (10.1016/0092-8674(90)90590-B) / CELL (1990)
10.1126/science.2588006
10.1126/science.1411535
QIN, X.Q., IDENTIFICATION OF A GROWTH SUPPRESSION DOMAIN WITHIN THE RETINOBLASTOMA GENE-PRODUCT, GENES & DEVELOPMENT 6: 953 (1992). (10.1101/gad.6.6.953) / GENES & DEVELOPMENT (1992)
QIN X.Q. unpublished data.
SHAN, B, MOLECULAR-CLONING OF CELLULAR GENES ENCODING RETINOBLASTOMA-ASSOCIATED PROTEINS - IDENTIFICATION OF A GENE WITH PROPERTIES OF THE TRANSCRIPTION FACTOR-E2F, MOLECULAR AND CELLULAR BIOLOGY 12: 5620 (1992). / MOLECULAR AND CELLULAR BIOLOGY (1992)
SHIRODKAR, S, THE TRANSCRIPTION FACTOR E2F INTERACTS WITH THE RETINOBLASTOMA PRODUCT AND A P107-CYCLIN-A COMPLEX IN A CELL CYCLE-REGULATED MANNER, CELL 68: 157 (1992). (10.1016/0092-8674(92)90214-W) / CELL (1992)
SHIRODKAR S unpublished data.

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:05 p.m.)
Deposited	1 year, 7 months ago (Jan. 15, 2024, 5:43 a.m.)
Indexed	6 days, 23 hours ago (Aug. 20, 2025, 9:21 a.m.)
Issued	31 years, 8 months ago (Dec. 3, 1993)
Published	31 years, 8 months ago (Dec. 3, 1993)
Published Print	31 years, 8 months ago (Dec. 3, 1993)

Funders 0

None

BibTeX

@article{Krek_1993, title={Binding to DNA and the Retinoblastoma Gene Product Promoted by Complex Formation of Different E2F Family Members}, volume={262}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8248803}, DOI={10.1126/science.8248803}, number={5139}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Krek, Wilhelm and Livingston, David M. and Shirodkar, Suman}, year={1993}, month=dec, pages={1557–1560} }

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