DOI: 10.1126/science.8248779. A Switch Between Two-, Three-, and Four-stranded Coiled Coils in GCN4 Leucine Zipper Mutants

A Switch Between Two-, Three-, and Four-stranded Coiled Coils in GCN4 Leucine Zipper Mutants

10.1126/science.8248779

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Coiled-coil sequences in proteins consist of heptad repeats containing two characteristic hydrophobic positions. The role of these buried hydrophobic residues in determining the structures of coiled coils was investigated by studying mutants of the GCN4 leucine zipper. When sets of buried residues were altered, two-, three-, and four-helix structures were formed. The x-ray crystal structure of the tetramer revealed a parallel, four-stranded coiled coil. In the tetramer conformation, the local packing geometry of the two hydrophobic positions in the heptad repeat is reversed relative to that in the dimer. These studies demonstrate that conserved, buried residues in the GCN4 leucine zipper direct dimer formation. In contrast to proposals that the pattern of hydrophobic and polar amino acids in a protein sequence is sufficient to determine three-dimensional structure, the shapes of buried side chains in coiled coils are essential determinants of the global fold.

Bibliography

Harbury, P. B., Zhang, T., Kim, P. S., & Alber, T. (1993). A Switch Between Two-, Three-, and Four-stranded Coiled Coils in GCN4 Leucine Zipper Mutants. Science, 262(5138), 1401â1407.

Authors 4

Pehr B. Harbury (first)
Tao Zhang (additional)
Peter S. Kim (additional)
Tom Alber (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:05 p.m.)
Deposited	1 year, 7 months ago (Jan. 15, 2024, 5:40 a.m.)
Indexed	2 weeks, 2 days ago (Aug. 7, 2025, 4:55 p.m.)
Issued	31 years, 8 months ago (Nov. 26, 1993)
Published	31 years, 8 months ago (Nov. 26, 1993)
Published Print	31 years, 8 months ago (Nov. 26, 1993)

Funders 0

None

BibTeX

@article{Harbury_1993, title={A Switch Between Two-, Three-, and Four-stranded Coiled Coils in GCN4 Leucine Zipper Mutants}, volume={262}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8248779}, DOI={10.1126/science.8248779}, number={5138}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Harbury, Pehr B. and Zhang, Tao and Kim, Peter S. and Alber, Tom}, year={1993}, month=nov, pages={1401–1407} }

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