DOI: 10.1126/science.8211143. Regional Codon Randomization: Defining a TATA-Binding Protein Surface Required for RNA Polymerase III Transcription

Regional Codon Randomization: Defining a TATA-Binding Protein Surface Required for RNA Polymerase III Transcription

10.1126/science.8211143

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The TATA-binding protein (TBP) is required for transcription by all three nuclear RNA polymerases. TBP was subjected to regional codon randomization, a codon-based mutagenesis method that generates complex yet compact protein libraries. Analysis of 186 temperature-sensitive TBP mutants yielded 65 specifically defective in transcription by RNA polymerase III (Pol III). These mutants map to a limited TBP surface that may interact with Tds4, a component of the Pol III transcription factor TFIIIB. Strains that contain the Pol III-defective derivatives have increased amounts of messenger RNA, which suggests that competition among TBP-interacting factors for limiting quantities of TBP determines the ratio of Pol II and Pol III transcription in vivo.

Bibliography

Cormack, B. P., & Struhl, K. (1993). Regional Codon Randomization: Defining a TATA-Binding Protein Surface Required for RNA Polymerase III Transcription. Science, 262(5131), 244â248.

Authors 2

Brendan P. Cormack (first)
Kevin Struhl (additional)

References 24 Referenced 78

BROU, C, DISTINCT TFIID COMPLEXES MEDIATE THE EFFECT OF DIFFERENT TRANSCRIPTIONAL ACTIVATORS, EMBO JOURNAL 12: 489 (1993). (10.1002/j.1460-2075.1993.tb05681.x) / EMBO JOURNAL (1993)
BURATOWSKI, S, A SUPPRESSOR OF TBP MUTATIONS ENCODES AN RNA POLYMERASE-III TRANSCRIPTION FACTOR WITH HOMOLOGY TO TFIIB, CELL 71: 221 (1992). (10.1016/0092-8674(92)90351-C) / CELL (1992)
10.1126/science.1546314
COLBERT, T, A YEAST TFIIB-RELATED FACTOR INVOLVED IN RNA POLYMERASE-III TRANSCRIPTION, GENES & DEVELOPMENT 6: 1940 (1992). (10.1101/gad.6.10.1940) / GENES & DEVELOPMENT (1992)
COMAI, L, THE TATA-BINDING PROTEIN AND ASSOCIATED FACTORS ARE INTEGRAL COMPONENTS OF THE RNA POLYMERASE-I TRANSCRIPTION FACTOR, SL1, CELL 68: 965 (1992). (10.1016/0092-8674(92)90039-F) / CELL (1992)
CORMACK, B.P., FUNCTIONAL DIFFERENCES BETWEEN YEAST AND HUMAN TFIID ARE LOCALIZED TO THE HIGHLY CONSERVED REGION, CELL 65: 341 (1991). (10.1016/0092-8674(91)90167-W) / CELL (1991)
CORMACK, B.P., THE TATA-BINDING PROTEIN IS REQUIRED FOR TRANSCRIPTION BY ALL 3 NUCLEAR-RNA POLYMERASES IN YEAST-CELLS, CELL 69: 685 (1992). (10.1016/0092-8674(92)90232-2) / CELL (1992)
DYNLACHT, B.D., ISOLATION OF COACTIVATORS ASSOCIATED WITH THE TATA-BINDING PROTEIN THAT MEDIATE TRANSCRIPTIONAL ACTIVATION, CELL 66: 563 (1991). (10.1016/0092-8674(81)90019-2) / CELL (1991)
EISENMANN, D.M., SPT3 INTERACTS WITH TFIID TO ALLOW NORMAL TRANSCRIPTION IN SACCHAROMYCES-CEREVISIAE, GENES & DEVELOPMENT 6: 1319 (1992). (10.1101/gad.6.7.1319) / GENES & DEVELOPMENT (1992)
GLASER, S. M., JOURNAL OF IMMUNOLOGY 149: 3903 (1992). (10.4049/jimmunol.149.12.3903) / JOURNAL OF IMMUNOLOGY (1992)
KASSAVETIS, G.A., THE ROLE OF THE TATA-BINDING PROTEIN IN THE ASSEMBLY AND FUNCTION OF THE MULTISUBUNIT YEAST RNA POLYMERASE-III TRANSCRIPTION FACTOR, TFIIIB, CELL 71: 1055 (1992). (10.1016/0092-8674(92)90399-W) / CELL (1992)
LEE, D.K., TFIIA INDUCES CONFORMATIONAL-CHANGES IN TFIID VIA INTERACTIONS WITH THE BASIC REPEAT, MOLECULAR AND CELLULAR BIOLOGY 12: 5189 (1992). / MOLECULAR AND CELLULAR BIOLOGY (1992)
Lee, W. S., Cell 67: 365 (1991). (10.1016/0092-8674(91)90188-5) / Cell (1991)
LOBO, S.M., A TBP COMPLEX ESSENTIAL FOR TRANSCRIPTION FROM TATA-LESS BUT NOT TATA-CONTAINING RNA POLYMERASE-III PROMOTERS IS PART OF THE TFIIIB FRACTION, CELL 71: 1029 (1992). (10.1016/0092-8674(92)90397-U) / CELL (1992)
LOPEZDELEON, A, PCF4 ENCODES AN RNA POLYMERASE-III TRANSCRIPTION FACTOR WITH HOMOLOGY TO TFIIB, CELL 71: 211 (1992). (10.1016/0092-8674(92)90350-L) / CELL (1992)
NIKOLOV, D.B., CRYSTAL-STRUCTURE OF TFIID TATA-BOX BINDING-PROTEIN, NATURE 360: 40 (1992). (10.1038/360040a0) / NATURE (1992)
SCHULTZ, M.C., VARIANTS OF THE TATA-BINDING PROTEIN CAN DISTINGUISH SUBSETS OF RNA POLYMERASE-I, POLYMERASE-II, AND POLYMERASE-III PROMOTERS, CELL 69: 697 (1992). (10.1016/0092-8674(92)90233-3) / CELL (1992)
TAGGART, AKP, THE TATA-BINDING PROTEIN AND ASSOCIATED FACTORS ARE COMPONENTS OF POL-III TRANSCRIPTION FACTOR TFIIIB, CELL 71: 1015 (1992). (10.1016/0092-8674(92)90396-T) / CELL (1992)
TAKADA, R, IDENTIFICATION OF HUMAN TFIID COMPONENTS AND DIRECT INTERACTION BETWEEN A 250-KDA POLYPEPTIDE AND THE TATA BOX-BINDING PROTEIN (TFIID-TAU), PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 89: 11809 (1992). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1992)
THOMPSON, C.M., A MULTISUBUNIT COMPLEX-ASSOCIATED WITH THE RNA POLYMERASE-II CTD AND TATA-BINDING PROTEIN IN YEAST, CELL 73: 1361 (1993). (10.1016/0092-8674(93)90362-T) / CELL (1993)
TIMMERS, HTM, THE MAMMALIAN TFIID PROTEIN IS PRESENT IN 2 FUNCTIONALLY DISTINCT COMPLEXES, GENES & DEVELOPMENT 5: 1946 (1991). (10.1101/gad.5.11.1946) / GENES & DEVELOPMENT (1991)
WHITE, R.J., MECHANISM OF TATA-BINDING PROTEIN RECRUITMENT TO A TATA-LESS CLASS-III PROMOTER, CELL 71: 1041 (1992). (10.1016/0092-8674(92)90398-V) / CELL (1992)
WHITE, R.J., A ROLE FOR THE TATA-BOX-BINDING PROTEIN-COMPONENT OF THE TRANSCRIPTION FACTOR-IID COMPLEX AS A GENERAL RNA POLYMERASE-III TRANSCRIPTION FACTOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 89: 1949 (1992). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1992)
ZHOU, Q, HOLO TFIID SUPPORTS TRANSCRIPTIONAL STIMULATION BY DIVERSE ACTIVATORS AND FROM A TATA-LESS PROMOTER, GENES & DEVELOPMENT 6: 1964 (1992). (10.1101/gad.6.10.1964) / GENES & DEVELOPMENT (1992)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:05 p.m.)
Deposited	1 year, 7 months ago (Jan. 15, 2024, 5:41 a.m.)
Indexed	3 months, 1 week ago (May 20, 2025, 9:16 a.m.)
Issued	31 years, 10 months ago (Oct. 8, 1993)
Published	31 years, 10 months ago (Oct. 8, 1993)
Published Print	31 years, 10 months ago (Oct. 8, 1993)

Funders 0

None

BibTeX

@article{Cormack_1993, title={Regional Codon Randomization: Defining a TATA-Binding Protein Surface Required for RNA Polymerase III Transcription}, volume={262}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8211143}, DOI={10.1126/science.8211143}, number={5131}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Cormack, Brendan P. and Struhl, Kevin}, year={1993}, month=oct, pages={244–248} }

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