Specification of Pore Properties by the Carboxyl Terminus of Inwardly Rectifying K + Channels
10.1126/science.8171340
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Inwardly rectifying potassium (K + ) channels (IRKs) maintain the resting membrane potential of cells and permit prolonged depolarization, such as during the cardiac action potential. Inward rectification may result from block of the ion conduction pore by intracellular magnesium (Mg i 2+ ). Two members of this family, IRK1 and ROMK1, which share 40 percent amino acid identity, differ markedly in single-channel K + conductance and sensitivity to block by Mg i 2+ . The conserved H 5 regions were hypothesized to determine these pore properties because they have this function in voltage-dependent K + channels and in cyclic nucleotide-gated channels. However, exchange of the H 5 region between IRK1 and ROMK1 had no effect on rectification and little or no effect on K + conductance. By contrast, exchange of the amino- and carboxyl-terminal regions together transferred Mg 2+ blockade and K + conductance of IRK1 to ROMK1. Exchange of the carboxyl but not the amino terminus had a similar effect. Therefore, the carboxyl terminus appears to have a major role in specifying the pore properties of IRKs.

Bibliography

Taglialatela, M., Wible, B. A., Caporaso, R., & Brown, A. M. (1994). Specification of Pore Properties by the Carboxyl Terminus of Inwardly Rectifying K + Channels. Science, 264(5160), 844–847.

Authors 4
  1. Maurizio Taglialatela (first)
  2. Barbara A. Wible (additional)
  3. Roberta Caporaso (additional)
  4. Arthur M. Brown (additional)
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Dates
Type When
Created 18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited 1 year, 7 months ago (Jan. 15, 2024, 12:32 a.m.)
Indexed 1 week ago (Aug. 23, 2025, 1:02 a.m.)
Issued 31 years, 3 months ago (May 6, 1994)
Published 31 years, 3 months ago (May 6, 1994)
Published Print 31 years, 3 months ago (May 6, 1994)
Funders 0

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@article{Taglialatela_1994, title={Specification of Pore Properties by the Carboxyl Terminus of Inwardly Rectifying K + Channels}, volume={264}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8171340}, DOI={10.1126/science.8171340}, number={5160}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Taglialatela, Maurizio and Wible, Barbara A. and Caporaso, Roberta and Brown, Arthur M.}, year={1994}, month=may, pages={844–847} }