DOI: 10.1126/science.8160014. <i>Bacillus subtilis</i> spoIIIE Protein Required for DNA Segregation During Asymmetric Cell Division

Bacillus subtilis spoIIIE Protein Required for DNA Segregation During Asymmetric Cell Division

10.1126/science.8160014

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Sporulation in Bacillus subtilis begins with an asymmetric cell division, producing a smaller prespore and a larger mother cell, both of which contain intact copies of the chromosome. The spoIIIE gene is required for chromosome segregation into the prespore compartment. The effects of the spoIIIE36 mutation on σ F -dependent transcription are an indirect consequence of the failure of certain genes to enter the cellular compartment in which their transcription factor has become active. SpoIIIE may also be required to prevent σ F from becoming active in the mother cell.

Bibliography

Wu, L. J., & Errington, J. (1994). Bacillus subtilis spoIIIE Protein Required for DNA Segregation During Asymmetric Cell Division. Science, 264(5158), 572â575.

Authors 2

Ling Juan Wu (first)
Jeffery Errington (additional)

References 45 Referenced 271

ALLMANSBERGER R unpublished data.
Anagnostopoulos, C., Bacillus subtilis and Other Gram Positive Bacteria: Biochemistry, Physiology and Molecular Genetics: 425 (1993). / Bacillus subtilis and Other Gram Positive Bacteria: Biochemistry, Physiology and Molecular Genetics (1993)
BARER M.R. unpublished data.
BEALL, B, FTSZ IN BACILLUS-SUBTILIS IS REQUIRED FOR VEGETATIVE SEPTATION AND FOR ASYMMETRIC SEPTATION DURING SPORULATION, GENES & DEVELOPMENT 5: 447 (1991). (10.1101/gad.5.3.447) / GENES & DEVELOPMENT (1991)
BENJAMIN, P, INITIATION OF DNA-REPLICATION INVITRO BY A DNA MEMBRANE COMPLEX EXTRACTED FROM BACILLUS-SUBTILIS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 80: 6214 (1983). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1983)
BUTLER, P.D., NUCLEOTIDE-SEQUENCE OF THE SPORULATION OPERON, SPOIIIE, OF BACILLUS-SUBTILIS, JOURNAL OF GENERAL MICROBIOLOGY 133: 2359 (1987). / JOURNAL OF GENERAL MICROBIOLOGY (1987)
BYLUND, J.E., AXIAL FILAMENT FORMATION IN BACILLUS-SUBTILIS - INDUCTION OF NUCLEOIDS OF INCREASING LENGTH AFTER ADDITION OF CHLORAMPHENICOL TO EXPONENTIAL-PHASE CULTURES APPROACHING STATIONARY PHASE, JOURNAL OF BACTERIOLOGY 175: 1886 (1993). (10.1128/jb.175.7.1886-1890.1993) / JOURNAL OF BACTERIOLOGY (1993)
CUTTING, S, A FORESPORE CHECKPOINT FOR MOTHER CELL GENE-EXPRESSION DURING DEVELOPMENT IN BACILLUS-SUBTILIS, CELL 62: 239 (1990). (10.1016/0092-8674(90)90362-I) / CELL (1990)
DANIEL, R.A., CLONING, DNA-SEQUENCE, FUNCTIONAL-ANALYSIS AND TRANSCRIPTIONAL REGULATION OF THE GENES ENCODING DIPICOLINIC ACID SYNTHETASE REQUIRED FOR SPORULATION IN BACILLUS-SUBTILIS, JOURNAL OF MOLECULAR BIOLOGY 232: 468 (1993). (10.1006/jmbi.1993.1403) / JOURNAL OF MOLECULAR BIOLOGY (1993)
EAST, A.K., A NEW BACTERIOPHAGE VECTOR FOR CLONING IN BACILLUS-SUBTILIS AND THE USE OF PHI-105 FOR PROTEIN-SYNTHESIS IN MAXICELLS, GENE 81: 35 (1989). (10.1016/0378-1119(89)90334-X) / GENE (1989)
ERRINGTON, J, USE OF A LACZ GENE FUSION TO DETERMINE THE DEPENDENCE PATTERN OF SPORULATION OPERON SPOIIA IN SPO MUTANTS OF BACILLUS-SUBTILIS, JOURNAL OF GENERAL MICROBIOLOGY 132: 2967 (1986). / JOURNAL OF GENERAL MICROBIOLOGY (1986)
ERRINGTON, J, BACILLUS-SUBTILIS SPORULATION - REGULATION OF GENE-EXPRESSION AND CONTROL OF MORPHOGENESIS, MICROBIOLOGICAL REVIEWS 57: 1 (1993). (10.1128/mr.57.1.1-33.1993) / MICROBIOLOGICAL REVIEWS (1993)
FOULGER, D, THE ROLE OF THE SPORULATION GENE SPOIIIE IN THE REGULATION OF PRESPORE-SPECIFIC GENE-EXPRESSION IN BACILLUS-SUBTILIS, MOLECULAR MICROBIOLOGY 3: 1247 (1989). (10.1111/j.1365-2958.1989.tb00275.x) / MOLECULAR MICROBIOLOGY (1989)
Harlow, E., Antibodies: A Laboratory Manual: 53 (1988). / Antibodies: A Laboratory Manual (1988)
HIRAGA, S, CHROMOSOME AND PLASMID PARTITION IN ESCHERICHIA-COLI, ANNUAL REVIEW OF BIOCHEMISTRY 61: 283 (1992). (10.1146/annurev.bi.61.070192.001435) / ANNUAL REVIEW OF BIOCHEMISTRY (1992)
10.1016/0092-8674(92)90468-R
HUISMAN, O, AN INDUCIBLE DNA REPLICATION-CELL DIVISION COUPLING MECHANISM IN ESCHERICHIA-COLI, NATURE 290: 797 (1981). (10.1038/290797a0) / NATURE (1981)
ILLING, N, GENETIC-REGULATION OF MORPHOGENESIS IN BACILLUS-SUBTILIS - ROLES OF SIGMA-E AND SIGMA-F IN PRESPORE ENGULFMENT, JOURNAL OF BACTERIOLOGY 173: 3159 (1991). (10.1128/jb.173.10.3159-3169.1991) / JOURNAL OF BACTERIOLOGY (1991)
JAFFE, A, SOS-INDEPENDENT COUPLING BETWEEN DNA-REPLICATION AND CELL-DIVISION IN ESCHERICHIA-COLI, JOURNAL OF BACTERIOLOGY 165: 66 (1986). (10.1128/jb.165.1.66-71.1986) / JOURNAL OF BACTERIOLOGY (1986)
KARMAZYNCAMPELL.C, TANDEM GENES ENCODING SIGMA-FACTORS FOR CONSECUTIVE STEPS OF DEVELOPMENT IN BACILLUS-SUBTILIS, GENES & DEVELOPMENT 3: 150 (1989). (10.1101/gad.3.2.150) / GENES & DEVELOPMENT (1989)
LAFFAN, J.J., ORIGIN-SPECIFIC DNA-BINDING MEMBRANE-ASSOCIATED PROTEIN MAY BE INVOLVED IN REPRESSION OF INITIATION OF DNA-REPLICATION IN BACILLUS-SUBTILIS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 7452 (1988). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1988)
Lewis, P. J., Proceedings of the National Academy of Sciences of the United States of America 91: 3849 (1994). / Proceedings of the National Academy of Sciences of the United States of America (1994)
LEWIS P.J. unpublished data.
LOBNEROLESEN, A, CHROMOSOME PARTITIONING IN ESCHERICHIA-COLI, JOURNAL OF BACTERIOLOGY 174: 7883 (1992). (10.1128/jb.174.24.7883-7889.1992) / JOURNAL OF BACTERIOLOGY (1992)
LOSICK, R, GENETICS OF ENDOSPORE FORMATION IN BACILLUS-SUBTILIS, ANNUAL REVIEW OF GENETICS 20: 625 (1986). (10.1146/annurev.ge.20.120186.003205) / ANNUAL REVIEW OF GENETICS (1986)
LOSICK, R, CRISSCROSS REGULATION OF CELL-TYPE-SPECIFIC GENE-EXPRESSION DURING DEVELOPMENT IN BACILLUS-SUBTILIS, NATURE 355: 601 (1992). (10.1038/355601a0) / NATURE (1992)
MARGOLIS, P, ESTABLISHMENT OF CELL TYPE BY COMPARTMENTALIZED ACTIVATION OF A TRANSCRIPTION FACTOR, SCIENCE 254: 562 (1991). (10.1126/science.1948031) / SCIENCE (1991)
MCGINNESS, T, DIVISION SEPTATION IN THE ABSENCE OF CHROMOSOME TERMINATION IN BACILLUS-SUBTILIS, JOURNAL OF MOLECULAR BIOLOGY 134: 251 (1979). (10.1016/0022-2836(79)90035-4) / JOURNAL OF MOLECULAR BIOLOGY (1979)
NIKI, H, THE NEW GENE MUKB CODES FOR A 177 KD PROTEIN WITH COILED-COIL DOMAINS INVOLVED IN CHROMOSOME PARTITIONING OF ESCHERICHIA-COLI, EMBO JOURNAL 10: 183 (1991). (10.1002/j.1460-2075.1991.tb07935.x) / EMBO JOURNAL (1991)
PIGGOT, P.J., GENETIC ASPECTS OF BACTERIAL ENDOSPORE FORMATION, BACTERIOLOGICAL REVIEWS 40: 908 (1976). (10.1128/br.40.4.908-962.1976) / BACTERIOLOGICAL REVIEWS (1976)
ROELS, S, CHARACTERIZATION OF SPOIVA, A SPORULATION GENE INVOLVED IN COAT MORPHOGENESIS IN BACILLUS-SUBTILIS, JOURNAL OF BACTERIOLOGY 174: 575 (1992). (10.1128/jb.174.2.575-585.1992) / JOURNAL OF BACTERIOLOGY (1992)
RYTER, A, ETUDE MORPHOLOGIQUE DE LA SPORULATION DE BACILLUS SUBTILIS, ANNALES DE L INSTITUT PASTEUR 108: 40 (1965). / ANNALES DE L INSTITUT PASTEUR (1965)
SETLOW, B, CONDENSATION OF THE FORESPORE NUCLEOID EARLY IN SPORULATION OF BACILLUS SPECIES, JOURNAL OF BACTERIOLOGY 173: 6270 (1991). (10.1128/jb.173.19.6270-6278.1991) / JOURNAL OF BACTERIOLOGY (1991)
SETLOW P unpublished data.
SMITH, D.B., SINGLE-STEP PURIFICATION OF POLYPEPTIDES EXPRESSED IN ESCHERICHIA-COLI AS FUSIONS WITH GLUTATHIONE S-TRANSFERASE, GENE 67: 31 (1988). (10.1016/0378-1119(88)90005-4) / GENE (1988)
STERLINI, J.M., COMMITMENT TO SPORULATION IN BACILLUS SUBTILIS AND ITS RELATIONSHIP TO DEVELOPMENT OF ACTINOMYCIN RESISTANCE, BIOCHEMICAL JOURNAL 113: 29 (1969). (10.1042/bj1130029) / BIOCHEMICAL JOURNAL (1969)
STEVENS, C.M., CHARACTERIZATION OF A SPORULATION GENE, SPOIVA, INVOLVED IN SPORE COAT MORPHOGENESIS IN BACILLUS-SUBTILIS, JOURNAL OF BACTERIOLOGY 174: 586 (1992). (10.1128/jb.174.2.586-594.1992) / JOURNAL OF BACTERIOLOGY (1992)
STRAGIER, P, PROCESSING OF A SPORULATION SIGMA FACTOR IN BACILLUS-SUBTILIS - HOW MORPHOLOGICAL STRUCTURE COULD CONTROL GENE-EXPRESSION, CELL 52: 697 (1988). (10.1016/0092-8674(88)90407-2) / CELL (1988)
Stragier, P., Regulation of Prokaryotic Development: A Structural and Functional Analysis of Sporulation and Germination: 243 (1989). / Regulation of Prokaryotic Development: A Structural and Functional Analysis of Sporulation and Germination (1989)
SUN, D.X., CONTROL OF TRANSCRIPTION OF THE BACILLUS-SUBTILIS SPOIIIG GENE, WHICH CODES FOR THE FORESPORE-SPECIFIC TRANSCRIPTION FACTOR-SIGMA-G, JOURNAL OF BACTERIOLOGY 173: 2977 (1991). (10.1128/jb.173.9.2977-2984.1991) / JOURNAL OF BACTERIOLOGY (1991)
SUN, D.X., EFFECT OF CHROMOSOME LOCATION OF BACILLUS-SUBTILIS FORESPORE GENES ON THEIR SPO GENE DEPENDENCE AND TRANSCRIPTION BY E-SIGMA-F - IDENTIFICATION OF FEATURES OF GOOD E-SIGMA-F-DEPENDENT PROMOTERS, JOURNAL OF BACTERIOLOGY 173: 7867 (1991). (10.1128/jb.173.24.7867-7874.1991) / JOURNAL OF BACTERIOLOGY (1991)
SUSSMAN, M.D., CLONING, NUCLEOTIDE-SEQUENCE, AND REGULATION OF THE BACILLUS-SUBTILIS GPR GENE, WHICH CODES FOR THE PROTEASE THAT INITIATES DEGRADATION OF SMALL, ACID-SOLUBLE PROTEINS DURING SPORE GERMINATION, JOURNAL OF BACTERIOLOGY 173: 291 (1991). (10.1128/jb.173.1.291-300.1991) / JOURNAL OF BACTERIOLOGY (1991)
Van Iterson, W., Microbiology 1976: 128 (1976). / Microbiology 1976 (1976)
WINSTON, S, DNA-MEMBRANE ASSOCIATION IS NECESSARY FOR INITIATION OF CHROMOSOMAL AND PLASMID REPLICATION IN BACILLUS-SUBTILIS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 77: 2834 (1980). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1980)
WU L.J. unpublished data.

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited	1 year, 7 months ago (Jan. 15, 2024, 12:33 a.m.)
Indexed	3 months, 1 week ago (May 18, 2025, 10:24 a.m.)
Issued	31 years, 4 months ago (April 22, 1994)
Published	31 years, 4 months ago (April 22, 1994)
Published Print	31 years, 4 months ago (April 22, 1994)

Funders 0

None

BibTeX

@article{Wu_1994, title={Bacillus subtilis spoIIIE Protein Required for DNA Segregation During Asymmetric Cell Division}, volume={264}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8160014}, DOI={10.1126/science.8160014}, number={5158}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Wu, Ling Juan and Errington, Jeffery}, year={1994}, month=apr, pages={572–575} }

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      "DOI": "10.1146/annurev.ge.20.120186.003205",
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      "journal-title": "ANNUAL REVIEW OF GENETICS"
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    {
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      "doi-asserted-by": "crossref",
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      "DOI": "10.1038/355601a0",
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    },
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      "first-page": "562",
      "DOI": "10.1126/science.1948031",
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      "journal-title": "SCIENCE"
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      "journal-title": "JOURNAL OF MOLECULAR BIOLOGY"
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      "journal-title": "JOURNAL OF BACTERIOLOGY"
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      "journal-title": "ANNALES DE L INSTITUT PASTEUR"
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      "DOI": "10.1128/jb.173.19.6270-6278.1991",
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      "journal-title": "JOURNAL OF BACTERIOLOGY"
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      "DOI": "10.1016/0092-8674(88)90407-2",
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      "journal-title": "CELL"
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      "journal-title": "Regulation of Prokaryotic Development: A Structural and Functional Analysis of Sporulation and Germination"
    },
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