DOI: 10.1126/science.8153628. Adhesion Forces Between Individual Ligand-Receptor Pairs

Adhesion Forces Between Individual Ligand-Receptor Pairs

10.1126/science.8153628

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The adhesion force between the tip of an atomic force microscope cantilever derivatized with avidin and agarose beads functionalized with biotin, desthiobiotin, or iminobiotin was measured. Under conditions that allowed only a limited number of molecular pairs to interact, the force required to separate tip and bead was found to be quantized in integer multiples of 160 ± 20 piconewtons for biotin and 85 ± 15 piconewtons for iminobiotin. The measured force quanta are interpreted as the unbinding forces of individual molecular pairs.

Bibliography

Florin, E.-L., Moy, V. T., & Gaub, H. E. (1994). Adhesion Forces Between Individual Ligand-Receptor Pairs. Science, 264(5157), 415â417.

Authors 3

Ernst-Ludwig Florin (first)
Vincent T. Moy (additional)
Hermann E. Gaub (additional)

References 24 Referenced 1,627

ASHKIN, A, OPTICAL TRAPPING AND MANIPULATION OF SINGLE CELLS USING INFRARED-LASER BEAMS, NATURE 330: 769 (1987). (10.1038/330769a0) / NATURE (1987)
BINNIG, G, ATOMIC FORCE MICROSCOPE, PHYSICAL REVIEW LETTERS 56: 930 (1986). (10.1103/PhysRevLett.56.930) / PHYSICAL REVIEW LETTERS (1986)
DARST, S.A., 2-DIMENSIONAL CRYSTALS OF STREPTAVIDIN ON BIOTINYLATED LIPID LAYERS AND THEIR INTERACTIONS WITH BIOTINYLATED MACROMOLECULES, BIOPHYSICAL JOURNAL 59: 387 (1991). (10.1016/S0006-3495(91)82232-9) / BIOPHYSICAL JOURNAL (1991)
10.1126/science.2928794
EGGER, M, SYNTHETIC LIPID-ANCHORED RECEPTORS BASED ON THE BINDING-SITE OF A MONOCLONAL-ANTIBODY, BIOCHIMICA ET BIOPHYSICA ACTA 1104: 45 (1992). (10.1016/0005-2736(92)90130-E) / BIOCHIMICA ET BIOPHYSICA ACTA (1992)
EVANS, E, DETACHMENT OF AGGLUTININ-BONDED RED-BLOOD-CELLS .1. FORCES TO RUPTURE MOLECULAR-POINT ATTACHMENTS, BIOPHYSICAL JOURNAL 59: 838 (1991). (10.1016/S0006-3495(91)82296-2) / BIOPHYSICAL JOURNAL (1991)
Evans E. Studying Cell Adhesion (1994).
FLORIN, E.L., ATOMIC-FORCE MICROSCOPE WITH MAGNETIC FORCE MODULATION, REVIEW OF SCIENTIFIC INSTRUMENTS 65: 639 (1994). (10.1063/1.1145130) / REVIEW OF SCIENTIFIC INSTRUMENTS (1994)
FRAUENFELDER, H, CONFORMATIONAL SUBSTATES IN PROTEINS, ANNUAL REVIEW OF BIOPHYSICS AND BIOPHYSICAL CHEMISTRY 17: 451 (1988). (10.1146/annurev.bb.17.060188.002315) / ANNUAL REVIEW OF BIOPHYSICS AND BIOPHYSICAL CHEMISTRY (1988)
GREEN, N.M., ADV PROTEIN CHEM 29: 85 (1975). (10.1016/S0065-3233(08)60411-8) / ADV PROTEIN CHEM (1975)
HELM, C.A., MEASUREMENT OF LIGAND RECEPTOR INTERACTIONS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 8169 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
Israelachvili J. N. Intermolecular and Surface Forces 2 (1992).
KARRASCH, S, COVALENT BINDING OF BIOLOGICAL SAMPLES TO SOLID SUPPORTS FOR SCANNING PROBE MICROSCOPY IN BUFFER SOLUTION, BIOPHYSICAL JOURNAL 65: 2437 (1993). (10.1016/S0006-3495(93)81327-4) / BIOPHYSICAL JOURNAL (1993)
10.1126/science.8469975
LEE, G.U., SENSING DISCRETE STREPTAVIDIN BIOTIN INTERACTIONS WITH ATOMIC-FORCE MICROSCOPY, LANGMUIR 10: 354 (1994). (10.1021/la00014a003) / LANGMUIR (1994)
LIVNAH, O, 3-DIMENSIONAL STRUCTURES OF AVIDIN AND THE AVIDIN-BIOTIN COMPLEX, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 90: 5076 (1993). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1993)
MARCHAND, P, BINOMIAL SMOOTHING FILTER - A WAY TO AVOID SOME PITFALLS OF LEAST-SQUARES POLYNOMIAL SMOOTHING, REVIEW OF SCIENTIFIC INSTRUMENTS 54: 1034 (1983). (10.1063/1.1137498) / REVIEW OF SCIENTIFIC INSTRUMENTS (1983)
MOY V.T. unpublished data.
PETHICA, J. B., PHYSICA SCRIPTA 19: 61 (1987). / PHYSICA SCRIPTA (1987)
PIERCE M unpublished data.
10.1126/science.1411505
10.1126/science.1439819
10.1038/365721a0
10.1126/science.7684161

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited	1 year, 7 months ago (Jan. 15, 2024, 12:31 a.m.)
Indexed	17 hours, 23 minutes ago (Aug. 22, 2025, 12:47 a.m.)
Issued	31 years, 4 months ago (April 15, 1994)
Published	31 years, 4 months ago (April 15, 1994)
Published Print	31 years, 4 months ago (April 15, 1994)

Funders 0

None

BibTeX

@article{Florin_1994, title={Adhesion Forces Between Individual Ligand-Receptor Pairs}, volume={264}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8153628}, DOI={10.1126/science.8153628}, number={5157}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Florin, Ernst-Ludwig and Moy, Vincent T. and Gaub, Hermann E.}, year={1994}, month=apr, pages={415–417} }

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