DOI: 10.1126/science.8091213. Complementation by SR Proteins of Pre-mRNA Splicing Reactions Depleted of U1 snRNP

Complementation by SR Proteins of Pre-mRNA Splicing Reactions Depleted of U1 snRNP

10.1126/science.8091213

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Individual small nuclear ribonucleoproteins (snRNPs) U1, U2, and U4/U6 were removed from nuclear extracts of HeLa cells by antisense affinity depletion. Addition of a highly purified preparation of SR proteins fully restored splicing activity in reactions depleted of U1 snRNP but did not reconstitute splicing in reactions depleted of the other snRNPs. Affinity selection experiments revealed that spliceosomes lacking U1 snRNA formed in the U1 snRNP-depleted reactions reconstituted with SR proteins. Thus, high concentrations of SR proteins facilitate the assembly of precursor messenger RNA (pre-mRNA) into a spliceosome in the absence of interactions with U1 snRNP.

Bibliography

Crispino, J. D., Blencowe, B. J., & Sharp, P. A. (1994). Complementation by SR Proteins of Pre-mRNA Splicing Reactions Depleted of U1 snRNP. Science, 265(5180), 1866â1869.

Authors 3

John D. Crispino (first)
Benjamin J. Blencowe (additional)
Phillip A. Sharp (additional)

References 30 Referenced 103

BARABINO, SML, TARGETED SNRNP DEPLETION REVEALS AN ADDITIONAL ROLE FOR MAMMALIAN U1 SNRNP IN SPLICEOSOME ASSEMBLY, CELL 63: 293 (1990). (10.1016/0092-8674(90)90162-8) / CELL (1990)
CRISPINO J.D. unpublished data.
10.1093/nar/11.5.1475
FU, X.D., SPECIFIC COMMITMENT OF DIFFERENT PREMESSENGER RNAS TO SPLICING BY SINGLE SR PROTEINS, NATURE 365: 82 (1993). (10.1038/365082a0) / NATURE (1993)
FU, X.D., GENERAL SPLICING FACTORS SF2 AND SC35 HAVE EQUIVALENT ACTIVITIES INVITRO, AND BOTH AFFECT ALTERNATIVE 5' AND 3' SPLICE SITE SELECTION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 89: 11224 (1992). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1992)
GE, H, PRIMARY STRUCTURE OF THE HUMAN SPLICING FACTOR ASF REVEALS SIMILARITIES WITH DROSOPHILA REGULATORS, CELL 66: 373 (1991). (10.1016/0092-8674(91)90626-A) / CELL (1991)
GRABOWSKI, P.J., MESSENGER-RNA SPLICING INVITRO - AN EXCISED INTERVENING SEQUENCE AND A POTENTIAL INTERMEDIATE, CELL 37: 415 (1984). (10.1016/0092-8674(84)90372-6) / CELL (1984)
10.1126/science.3638792
10.1126/science.1853200
KANDELSLEWIS, S, ROLE OF U6 SNRNA IN 5' SPLICE-SITE SELECTION, SCIENCE 262: 2035 (1993). (10.1126/science.8266100) / SCIENCE (1993)
KOHTZ, J.D., PROTEIN-PROTEIN INTERACTIONS AND 5'-SPLICE-SITE RECOGNITION IN MAMMALIAN MESSENGER-RNA PRECURSORS, NATURE 368: 119 (1994). (10.1038/368119a0) / NATURE (1994)
KONARSKA, M.M., ELECTROPHORETIC SEPARATION OF COMPLEXES INVOLVED IN THE SPLICING OF PRECURSORS TO MESSENGER-RNAS, CELL 46: 845 (1986). (10.1016/0092-8674(86)90066-8) / CELL (1986)
KONARSKA, M.M., INTERACTIONS BETWEEN SMALL NUCLEAR RIBONUCLEOPROTEIN-PARTICLES IN FORMATION OF SPLICEOSOMES, CELL 49: 763 (1987). (10.1016/0092-8674(87)90614-3) / CELL (1987)
KONFORTI, B.B., DISRUPTION OF BASE-PAIRING BETWEEN THE 5' SPLICE-SITE AND THE 5' END OF U1 SNRNA IS REQUIRED FOR SPLICEOSOME ASSEMBLY, CELL 75: 863 (1993). (10.1016/0092-8674(93)90531-T) / CELL (1993)
KRAINER, A.R., FUNCTIONAL EXPRESSION OF CLONED HUMAN SPLICING FACTOR SF2 - HOMOLOGY TO RNA-BINDING PROTEINS, U1-70K, AND DROSOPHILA SPLICING REGULATORS, CELL 66: 383 (1991). (10.1016/0092-8674(91)90627-B) / CELL (1991)
10.1126/science.8266093
MICHAUD, S, AN ATP-INDEPENDENT COMPLEX COMMITS PRE-MESSENGER-RNA TO THE MAMMALIAN SPLICEOSOME ASSEMBLY PATHWAY, GENES & DEVELOPMENT 5: 2534 (1991). (10.1101/gad.5.12b.2534) / GENES & DEVELOPMENT (1991)
Moore, M. J., The RNA World: 303 (1993). / The RNA World (1993)
NILSEN, T.W., TRANSSPLICING OF NEMATODE PREMESSENGER RNA, ANNUAL REVIEW OF MICROBIOLOGY 47: 413 (1993). (10.1146/annurev.mi.47.100193.002213) / ANNUAL REVIEW OF MICROBIOLOGY (1993)
RYDER, U, SEQUENCE-SPECIFIC AFFINITY SELECTION OF MAMMALIAN SPLICING COMPLEXES, NUCLEIC ACIDS RESEARCH 18: 7373 (1990). (10.1093/nar/18.24.7373) / NUCLEIC ACIDS RESEARCH (1990)
SERAPHIN, B, IDENTIFICATION OF FUNCTIONAL U1 SNRNA PRE-MESSENGER RNA COMPLEXES COMMITTED TO SPLICEOSOME ASSEMBLY AND SPLICING, CELL 59: 349 (1989). (10.1016/0092-8674(89)90296-1) / CELL (1989)
SERAPHIN, B, A U1 SNRNA - PRE-MRNA BASE-PAIRING INTERACTION IS REQUIRED EARLY IN YEAST SPLICEOSOME ASSEMBLY BUT DOES NOT UNIQUELY DEFINE THE 5' CLEAVAGE SITE, EMBO JOURNAL 7: 2533 (1988). (10.1002/j.1460-2075.1988.tb03101.x) / EMBO JOURNAL (1988)
SILICIANO, P.G., 5' SPLICE SITE SELECTION IN YEAST - GENETIC ALTERATIONS IN BASE-PAIRING WITH U1 REVEAL ADDITIONAL REQUIREMENTS, GENES & DEVELOPMENT 2: 1258 (1988). (10.1101/gad.2.10.1258) / GENES & DEVELOPMENT (1988)
SONTHEIMER, E, SCIENCE 262: 1989 (1982). / SCIENCE (1982)
TARN W.Y. unpublished data.
WU, J.Y., SPECIFIC INTERACTIONS BETWEEN PROTEINS IMPLICATED IN SPLICE-SITE SELECTION AND REGULATED ALTERNATIVE SPLICING, CELL 75: 1061 (1993). (10.1016/0092-8674(93)90316-I) / CELL (1993)
ZAHLER, A.M., SR PROTEINS - A CONSERVED FAMILY OF PRE-MESSENGER-RNA SPLICING FACTORS, GENES & DEVELOPMENT 6: 837 (1992). (10.1101/gad.6.5.837) / GENES & DEVELOPMENT (1992)
Zahler, K. M., Molecular and Cellular Biology 13: 4023 (1993). / Molecular and Cellular Biology (1993)
10.1016/0092-8674(86)90064-4
ZUO, P, THE HUMAN SPLICING FACTOR ASF SF2 CAN SPECIFICALLY RECOGNIZE PREMESSENGER RNA 5' SPLICE SITES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 91: 3363 (1994). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1994)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 10:49 p.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 2:32 p.m.)
Issued	30 years, 11 months ago (Sept. 23, 1994)
Published	30 years, 11 months ago (Sept. 23, 1994)
Published Print	30 years, 11 months ago (Sept. 23, 1994)

Funders 0

None

BibTeX

@article{Crispino_1994, title={Complementation by SR Proteins of Pre-mRNA Splicing Reactions Depleted of U1 snRNP}, volume={265}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8091213}, DOI={10.1126/science.8091213}, number={5180}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Crispino, John D. and Blencowe, Benjamin J. and Sharp, Phillip A.}, year={1994}, month=sep, pages={1866–1869} }

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