DOI: 10.1126/science.8066464. Catalysis of ATP-Dependent Homologous DNA Pairing and Strand Exchange by Yeast RAD51 Protein

Catalysis of ATP-Dependent Homologous DNA Pairing and Strand Exchange by Yeast RAD51 Protein

10.1126/science.8066464

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The RAD51 gene of Saccharomyces cerevisiae is required for genetic recombination and DNA double-strand break repair. Here it is demonstrated that RAD51 protein pairs circular viral single-stranded DNA from φX 174 or M13 with its respective homologous linear double-stranded form. The product of synapsis between these DNA partners is further processed by RAD51 to yield nicked circular duplex DNA, which indicates that RAD51 can catalyze strand exchange. The pairing and strand exchange reaction requires adenosine triphosphate, a result consistent with the presence of a DNA-dependent adenosine triphosphatase activity in RAD51 protein. Thus, RAD51 is a eukaryotic recombination protein that can catalyze the strand exchange reaction.

Bibliography

Sung, P. (1994). Catalysis of ATP-Dependent Homologous DNA Pairing and Strand Exchange by Yeast RAD51 Protein. Science, 265(5176), 1241â1243.

Authors 1

Patrick Sung (first)

References 20 Referenced 672

ABOUSSEKHRA, A, SEMIDOMINANT SUPPRESSORS OF SRS2 HELICASE MUTATIONS OF SACCHAROMYCES-CEREVISIAE MAP IN THE RAD51 GENE, WHOSE SEQUENCE PREDICTS A PROTEIN WITH SIMILARITIES TO PROKARYOTIC RECA PROTEINS, MOLECULAR AND CELLULAR BIOLOGY 12: 3224 (1992). / MOLECULAR AND CELLULAR BIOLOGY (1992)
BASILE, G, NUCLEOTIDE-SEQUENCE AND TRANSCRIPTIONAL REGULATION OF THE YEAST RECOMBINATIONAL REPAIR GENE RAD51, MOLECULAR AND CELLULAR BIOLOGY 12: 3235 (1992). / MOLECULAR AND CELLULAR BIOLOGY (1992)
BEZZUBOVA, O, A CHICKEN RAD51 HOMOLOG IS EXPRESSED AT HIGH-LEVELS IN LYMPHOID AND REPRODUCTIVE-ORGANS, NUCLEIC ACIDS RESEARCH 21: 1577 (1993). (10.1093/nar/21.7.1577) / NUCLEIC ACIDS RESEARCH (1993)
10.1016/0092-8674(92)90446-J
BRILL, S.J., YEAST REPLICATION FACTOR-A FUNCTIONS IN THE UNWINDING OF THE SV40 ORIGIN OF DNA-REPLICATION, NATURE 342: 92 (1989). (10.1038/342092a0) / NATURE (1989)
GUZDER, S.N., RAD25 IS A DNA HELICASE REQUIRED FOR RNA REPAIR AND RNA-POLYMERASE-II TRANSCRIPTION, NATURE 369: 578 (1994). (10.1038/369578a0) / NATURE (1994)
KANS, J.A., NUCLEOTIDE-SEQUENCE OF THE RAD57 GENE OF SACCHAROMYCES-CEREVISIAE, GENE 105: 139 (1991). (10.1016/0378-1119(91)90527-I) / GENE (1991)
KLECKNER, N, MEIOTIC CHROMOSOME METABOLISM - ONE VIEW, COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 56: 729 (1991). (10.1101/SQB.1991.056.01.082) / COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY (1991)
MORITA, T, A MOUSE HOMOLOG OF THE ESCHERICHIA-COLI RECA AND SACCHAROMYCES-CEREVISIAE RAD51 GENES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 90: 6577 (1993). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1993)
10.1126/science.8456314
Petes, T. D., The Molecular and Cellular Biology of the Yeast Saccharomyces: Genome Dynamics, Protein Synthesis, and Energetics: 407 (1991). / The Molecular and Cellular Biology of the Yeast Saccharomyces: Genome Dynamics, Protein Synthesis, and Energetics (1991)
RADDING, C.M., HELICAL INTERACTIONS IN HOMOLOGOUS PAIRING AND STRAND EXCHANGE DRIVEN BY RECA PROTEIN, JOURNAL OF BIOLOGICAL CHEMISTRY 266: 5355 (1991). (10.1016/S0021-9258(19)67599-X) / JOURNAL OF BIOLOGICAL CHEMISTRY (1991)
ROCA, A.I., THE RECA PROTEIN - STRUCTURE AND FUNCTION, CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY 25: 415 (1990). (10.3109/10409239009090617) / CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY (1990)
SHINOHARA, A, RAD51 PROTEIN INVOLVED IN REPAIR AND RECOMBINATION IN SACCHAROMYCES-CEREVISIAE IS A RECA-LIKE PROTEIN, CELL 69: 457 (1992). (10.1016/0092-8674(92)90447-K) / CELL (1992)
SHINOHARA, A, CLONING OF HUMAN, MOUSE AND FISSION YEAST RECOMBINATION GENES HOMOLOGOUS TO RAD51 AND RECA, NATURE GENETICS 4: 239 (1993). (10.1038/ng0793-239) / NATURE GENETICS (1993)
STORY, R.M., STRUCTURAL RELATIONSHIP OF BACTERIAL RECA PROTEINS TO RECOMBINATION PROTEINS FROM BACTERIOPHAGE-T4 AND YEAST, SCIENCE 259: 1892 (1993). (10.1126/science.8456313) / SCIENCE (1993)
SUNG, P, HUMAN XERODERMA-PIGMENTOSUM GROUP-D GENE ENCODES A DNA HELICASE, NATURE 365: 852 (1993). (10.1038/365852a0) / NATURE (1993)
SUNG, P, THE RAD3 GENE OF SACCHAROMYCES-CEREVISIAE ENCODES A DNA-DEPENDENT ATPASE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84: 6045 (1987). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1987)
SUNG P unpublished data.
YOSHIMURA, Y, CLONING AND SEQUENCE OF THE HUMAN RECA-LIKE GENE CDNA, NUCLEIC ACIDS RESEARCH 21: 1665 (1993). (10.1093/nar/21.7.1665) / NUCLEIC ACIDS RESEARCH (1993)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:04 p.m.)
Indexed	2 weeks, 2 days ago (Aug. 19, 2025, 6:38 a.m.)
Issued	31 years ago (Aug. 26, 1994)
Published	31 years ago (Aug. 26, 1994)
Published Print	31 years ago (Aug. 26, 1994)

Funders 0

None

BibTeX

@article{Sung_1994, title={Catalysis of ATP-Dependent Homologous DNA Pairing and Strand Exchange by Yeast RAD51 Protein}, volume={265}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8066464}, DOI={10.1126/science.8066464}, number={5176}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Sung, Patrick}, year={1994}, month=aug, pages={1241–1243} }

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