DOI: 10.1126/science.8066460. Dependence of Enhancer-Mediated Transcription of the Immunoglobulin μ Gene on Nuclear Matrix Attachment Regions

Dependence of Enhancer-Mediated Transcription of the Immunoglobulin μ Gene on Nuclear Matrix Attachment Regions

10.1126/science.8066460

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Transcription of the immunoglobulin μ heavy chain locus is regulated by an intronic enhancer that is flanked on both sides by nuclear matrix attachment regions (MARs). These MARs have now been shown to be essential for transcription of a rearranged μ gene in transgenic B lymphocytes, but they were not required in stably transfected tissue culture cells. Normal rates of transcriptional initiation at a variable region promoter and the formation of an extended deoxyribonuclease I (DNase I)—sensitive chromatin domain were dependent on MARs, although DNase I hypersensitivity at the enhancer was detected in the absence of MARs. Thus, transcriptional activation of the μ gene during normal lymphoid development requires a synergistic collaboration between the enhancer and flanking MARs.

Bibliography

Forrester, W. C., van Genderen, C., Jenuwein, T., & Grosschedl, R. (1994). Dependence of Enhancer-Mediated Transcription of the Immunoglobulin Î¼ Gene on Nuclear Matrix Attachment Regions. Science, 265(5176), 1221â1225.

Authors 4

William C. Forrester (first)
Courtney van Genderen (additional)
Thomas Jenuwein (additional)
Rudolf Grosschedl (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:05 p.m.)
Indexed	3 weeks, 6 days ago (Aug. 5, 2025, 8:55 a.m.)
Issued	31 years ago (Aug. 26, 1994)
Published	31 years ago (Aug. 26, 1994)
Published Print	31 years ago (Aug. 26, 1994)

Funders 0

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BibTeX

@article{Forrester_1994, title={Dependence of Enhancer-Mediated Transcription of the Immunoglobulin μ Gene on Nuclear Matrix Attachment Regions}, volume={265}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8066460}, DOI={10.1126/science.8066460}, number={5176}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Forrester, William C. and van Genderen, Courtney and Jenuwein, Thomas and Grosschedl, Rudolf}, year={1994}, month=aug, pages={1221–1225} }

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