The Three-Dimensional Crystal Structure of the Catalytic Core of Cellobiohydrolase I from Trichoderma reesei
10.1126/science.8036495
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Cellulose is the major polysaccharide of plants where it plays a predominantly structural role. A variety of highly specialized microorganisms have evolved to produce enzymes that either synergistically or in complexes can carry out the complete hydrolysis of cellulose. The structure of the major cellobiohydrolase, CBHI, of the potent cellulolytic fungus Trichoderma reesei has been determined and refined to 1.8 angstrom resolution. The molecule contains a 40 angstrom long active site tunnel that may account for many of the previously poorly understood macroscopic properties of the enzyme and its interaction with solid cellulose. The active site residues were identified by solving the structure of the enzyme complexed with an oligosaccharide, o -iodobenzyl-1-thio-β-cellobioside. The three-dimensional structure is very similar to a family of bacterial β-glucanases with the main-chain topology of the plant legume lectins.

Bibliography

Divne, C., Ståhlberg, J., Reinikainen, T., Ruohonen, L., Pettersson, G., Knowles, J. K. C., Teeri, T. T., & Jones, T. A. (1994). The Three-Dimensional Crystal Structure of the Catalytic Core of Cellobiohydrolase I from Trichoderma reesei. Science, 265(5171), 524–528.

Authors 8
  1. Christina Divne (first)
  2. Jerry Ståhlberg (additional)
  3. Tapani Reinikainen (additional)
  4. Laura Ruohonen (additional)
  5. Göran Pettersson (additional)
  6. Jonathan K. C. Knowles (additional)
  7. Tuula T. Teeri (additional)
  8. T. Alwyn Jones (additional)
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Dates
Type When
Created 18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited 1 year, 7 months ago (Jan. 14, 2024, 11 p.m.)
Indexed 3 weeks, 6 days ago (July 28, 2025, 5:30 p.m.)
Issued 31 years, 1 month ago (July 22, 1994)
Published 31 years, 1 month ago (July 22, 1994)
Published Print 31 years, 1 month ago (July 22, 1994)
Funders 0

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@article{Divne_1994, title={The Three-Dimensional Crystal Structure of the Catalytic Core of Cellobiohydrolase I from Trichoderma reesei}, volume={265}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8036495}, DOI={10.1126/science.8036495}, number={5171}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Divne, Christina and Ståhlberg, Jerry and Reinikainen, Tapani and Ruohonen, Laura and Pettersson, Göran and Knowles, Jonathan K. C. and Teeri, Tuula T. and Jones, T. Alwyn}, year={1994}, month=jul, pages={524–528} }