DOI: 10.1126/science.8016656. Identification of the Target of a Transcription Activator Protein by Protein-Protein Photocrosslinking

Identification of the Target of a Transcription Activator Protein by Protein-Protein Photocrosslinking

10.1126/science.8016656

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Here it is shown, with the use of protein-protein photocrosslinking, that the carboxyl-terminal region of the α subunit of RNA polymerase (RNAP) is in direct physical proximity to the activating region of the catabolite gene activator protein (CAP) in the ternary complex of the lac promoter, RNAP, and CAP. These results strongly support the proposal that transcription activation by CAP involves protein-protein contact between the carboxyl-terminal region of the α subunit and the activating region of CAP.

Bibliography

Chen, Y., Ebright, Y. W., & Ebright, R. H. (1994). Identification of the Target of a Transcription Activator Protein by Protein-Protein Photocrosslinking. Science, 265(5168), 90â92.

Authors 3

Yan Chen (first)
Yon W. Ebright (additional)
Richard H. Ebright (additional)

References 25 Referenced 135

10.1093/nar/18.24.7243
EBRIGHT, R.H., TRANSCRIPTION ACTIVATION AT CLASS-I CAP-DEPENDENT PROMOTERS, MOLECULAR MICROBIOLOGY 8: 797 (1993). (10.1111/j.1365-2958.1993.tb01626.x) / MOLECULAR MICROBIOLOGY (1993)
10.1128/jb.173.16.5024-5029.1991
FRAKER, P.J., PROTEIN AND CELL-MEMBRANE IODINATIONS WITH A SPARINGLY SOLUBLE CHLORAMIDE, 1,3,4,6-TETRACHLORO-3A,6A-DIPHENYLGLYCOLURIL, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 80: 849 (1978). (10.1016/0006-291X(78)91322-0) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1978)
HAGER, D.A., USE OF MONO-Q HIGH-RESOLUTION ION-EXCHANGE CHROMATOGRAPHY TO OBTAIN HIGHLY PURE AND ACTIVE ESCHERICHIA-COLI RNA-POLYMERASE, BIOCHEMISTRY 29: 7890 (1990). (10.1021/bi00486a016) / BIOCHEMISTRY (1990)
HEYDUK, T, CAP INTERACTS WITH RNA-POLYMERASE IN SOLUTION IN THE ABSENCE OF PROMOTER DNA, NATURE 364: 548 (1993). (10.1038/364548a0) / NATURE (1993)
HU, J. C., MOLECULAR & GENERAL GENETICS 199: 7 (1985). (10.1007/BF00327502) / MOLECULAR & GENERAL GENETICS (1985)
IGARASHI, K, BIPARTITE FUNCTIONAL MAP OF THE ESCHERICHIA-COLI RNA POLYMERASE-ALPHA SUBUNIT - INVOLVEMENT OF THE C-TERMINAL REGION IN TRANSCRIPTION ACTIVATION BY CAMP-CRP, CELL 65: 1015 (1991). (10.1016/0092-8674(91)90553-B) / CELL (1991)
ISHIHAMA, A, PROTEIN-PROTEIN COMMUNICATION WITHIN THE TRANSCRIPTION APPARATUS, JOURNAL OF BACTERIOLOGY 175: 2483 (1993). (10.1128/jb.175.9.2483-2489.1993) / JOURNAL OF BACTERIOLOGY (1993)
KOLB, A, TRANSCRIPTIONAL REGULATION BY CAMP AND ITS RECEPTOR PROTEIN, ANNUAL REVIEW OF BIOCHEMISTRY 62: 749 (1993). (10.1146/annurev.bi.62.070193.003533) / ANNUAL REVIEW OF BIOCHEMISTRY (1993)
KOLB, A, ESCHERICHIA-COLI RNA-POLYMERASE, DELETED IN THE C-TERMINAL PART OF ITS ALPHA-SUBUNIT, INTERACTS DIFFERENTLY WITH THE CAMP-CRP COMPLEX AT THE LACP1 AND AT THE GALP1 PROMOTER, NUCLEIC ACIDS RESEARCH 21: 319 (1993). (10.1093/nar/21.2.319) / NUCLEIC ACIDS RESEARCH (1993)
KUMAR, J MOL BIOL 235: 405 (1994). (10.1006/jmbi.1994.1001) / J MOL BIOL (1994)
KUNKEL, T.A., EFFICIENT SITE-DIRECTED MUTAGENESIS USING URACIL-CONTAINING DNA, METHODS IN ENZYMOLOGY 204: 125 (1991). (10.1016/0076-6879(91)04008-C) / METHODS IN ENZYMOLOGY (1991)
10.1126/science.8272867
NIU W unpublished data.
OVCHINNIKOV, Y.A., PRIMARY STRUCTURE OF ALPHA-SUBUNIT OF DNA-DEPENDENT RNA-POLYMERASE FROM ESCHERICHIA-COLI, FEBS LETTERS 76: 108 (1977). (10.1016/0014-5793(77)80131-2) / FEBS LETTERS (1977)
RUSSO, F.D., ALPHA - THE CINDERELLA SUBUNIT OF RNA-POLYMERASE, JOURNAL OF BIOLOGICAL CHEMISTRY 267: 14515 (1992). (10.1016/S0021-9258(18)42065-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1992)
SARIS, CJM, HYDROXYLAMINE CLEAVAGE OF PROTEINS IN POLYACRYLAMIDE GELS, ANALYTICAL BIOCHEMISTRY 132: 54 (1983). (10.1016/0003-2697(83)90425-6) / ANALYTICAL BIOCHEMISTRY (1983)
SCHULTZ, S.C., CRYSTAL-STRUCTURE OF A CAP-DNA COMPLEX - THE DNA IS BENT BY 90-DEGREES, SCIENCE 253: 1001 (1991). (10.1126/science.1653449) / SCIENCE (1991)
TANG H unpublished data.
ZHANG, X. P., JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS 9: 463 (1991). (10.1080/07391102.1991.10507929) / JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS (1991)
ZHANG, X.P., CATABOLITE GENE ACTIVATOR PROTEIN (CAP) IS NOT AN ACIDIC ACTIVATING REGION TRANSCRIPTION ACTIVATOR PROTEIN - NEGATIVELY CHARGED AMINO-ACIDS OF CAP THAT ARE SOLVENT-ACCESSIBLE IN THE CAP-DNA COMPLEX PLAY NO ROLE IN TRANSCRIPTION ACTIVATION AT THE LAC PROMOTER, JOURNAL OF BIOLOGICAL CHEMISTRY 267: 8136 (1992). (10.1016/S0021-9258(18)42417-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1992)
ZHOU, Y.H., IDENTIFICATION OF THE FUNCTIONAL SUBUNIT OF A DIMERIC TRANSCRIPTION ACTIVATOR PROTEIN BY USE OF ORIENTED HETERODIMERS, CELL 73: 375 (1993). (10.1016/0092-8674(93)90236-J) / CELL (1993)
ZHOU, Y.H., IDENTIFICATION OF THE ACTIVATING REGION OF CATABOLITE GENE ACTIVATOR PROTEIN (CAP) - ISOLATION AND CHARACTERIZATION OF MUTANTS OF CAP SPECIFICALLY DEFECTIVE IN TRANSCRIPTION ACTIVATION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 90: 6081 (1993). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1993)
ZOU, C, MAPPING THE CAMP RECEPTOR PROTEIN CONTACT SITE ON THE ALPHA-SUBUNIT OF ESCHERICHIA-COLI RNA-POLYMERASE, MOLECULAR MICROBIOLOGY 6: 2599 (1992). (10.1111/j.1365-2958.1992.tb01437.x) / MOLECULAR MICROBIOLOGY (1992)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:08 p.m.)
Indexed	1 year, 3 months ago (May 24, 2024, 10:13 p.m.)
Issued	31 years, 1 month ago (July 1, 1994)
Published	31 years, 1 month ago (July 1, 1994)
Published Print	31 years, 1 month ago (July 1, 1994)

Funders 0

None

BibTeX

@article{Chen_1994, title={Identification of the Target of a Transcription Activator Protein by Protein-Protein Photocrosslinking}, volume={265}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8016656}, DOI={10.1126/science.8016656}, number={5168}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Chen, Yan and Ebright, Yon W. and Ebright, Richard H.}, year={1994}, month=jul, pages={90–92} }

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