DOI: 10.1126/science.8016655. Stimulation of GAL4 Derivative Binding to Nucleosomal DNA by the Yeast SWI/SNF Complex

Stimulation of GAL4 Derivative Binding to Nucleosomal DNA by the Yeast SWI/SNF Complex

10.1126/science.8016655

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The SWI/SNF protein complex is required for the enhancement of transcription by many transcriptional activators in yeast. Here it is shown that the purified SWI/SNF complex is composed of 10 subunits and includes the SWI1, SWI2/SNF2, SWI3, SNF5, and SNF6 gene products. The complex exhibited DNA-stimulated adenosine triphosphatase (ATPase) activity, but lacked helicase activity. The SWI/SNF complex caused a 10- to 30-fold stimulation in the binding of GAL4 derivatives to nucleosomal DNA in a reaction that required adenosine triphosphate (ATP) hydrolysis but was activation domain-independent. Stimulation of GAL4 binding by the complex was abolished by a mutant SWI2 subunit, and was increased by the presence of a histone-binding protein, nucleoplasmin. A direct ATP-dependent interaction between the SWI/SNF complex and nucleosomal DNA was detected. These observations suggest that a primary role of the SWI/SNF complex is to promote activator binding to nucleosomal DNA.

Bibliography

CÃ´tÃ©, J., Quinn, J., Workman, J. L., & Peterson, C. L. (1994). Stimulation of GAL4 Derivative Binding to Nucleosomal DNA by the Yeast SWI/SNF Complex. Science, 265(5168), 53â60.

Authors 4

Jacques Côté (first)
Janet Quinn (additional)
Jerry L. Workman (additional)
Craig L. Peterson (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:07 p.m.)
Indexed	2 days, 2 hours ago (Aug. 20, 2025, 8:32 a.m.)
Issued	31 years, 1 month ago (July 1, 1994)
Published	31 years, 1 month ago (July 1, 1994)
Published Print	31 years, 1 month ago (July 1, 1994)

Funders 0

None

BibTeX

@article{C_t__1994, title={Stimulation of GAL4 Derivative Binding to Nucleosomal DNA by the Yeast SWI/SNF Complex}, volume={265}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.8016655}, DOI={10.1126/science.8016655}, number={5168}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Côté, Jacques and Quinn, Janet and Workman, Jerry L. and Peterson, Craig L.}, year={1994}, month=jul, pages={53–60} }

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