DOI: 10.1126/science.7973731. The PCL2 (ORFD)-PHO85 Cyclin-Dependent Kinase Complex: a Cell Cycle Regulator in Yeast

The PCL2 (ORFD)-PHO85 Cyclin-Dependent Kinase Complex: a Cell Cycle Regulator in Yeast

10.1126/science.7973731

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Cyclin-dependent kinase (cdk) complexes are essential activators of cell cycle progression in all eukaryotes. In contrast to mammalian cells, in which multiple cdk's contribute to cell cycle regulation, the yeast cell cycle is largely controlled by the activity of a single cdk, CDC28. Analysis of the putative G 1 cyclin PCL2 (ORFD) identified a second cyclin-cdk complex that contributes to cell cycle progression in yeast. PCL2 interacted with the cdk PHO85 in vivo and in vitro and formed a kinase complex that had G 1 -periodic activity. Under genetic conditions in which the Start transition was compromised, PHO85 and its associated cyclin subunits were essential for cell cycle commitment. Because PHO85 and another cyclin-like molecule, PHO80, also take part in inorganic phosphate metabolism, this cdk enzyme may integrate responses to nutritional conditions with the cell cycle.

Bibliography

Measday, V., Moore, L., Ogas, J., Tyers, M., & Andrews, B. (1994). The PCL2 (ORFD)-PHO85 Cyclin-Dependent Kinase Complex: a Cell Cycle Regulator in Yeast. Science, 266(5189), 1391â1395.

Authors 5

Vivien Measday (first)
Lynda Moore (additional)
Joseph Ogas (additional)
Mike Tyers (additional)
Brenda Andrews (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:20 p.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 1:58 p.m.)
Issued	30 years, 9 months ago (Nov. 25, 1994)
Published	30 years, 9 months ago (Nov. 25, 1994)
Published Print	30 years, 9 months ago (Nov. 25, 1994)

Funders 0

None

BibTeX

@article{Measday_1994, title={The PCL2 (ORFD)-PHO85 Cyclin-Dependent Kinase Complex: a Cell Cycle Regulator in Yeast}, volume={266}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7973731}, DOI={10.1126/science.7973731}, number={5189}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Measday, Vivien and Moore, Lynda and Ogas, Joseph and Tyers, Mike and Andrews, Brenda}, year={1994}, month=nov, pages={1391–1395} }

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