DOI: 10.1126/science.7973708. The Role of Hsp70 in Conferring Unidirectionality on Protein Translocation into Mitochondria

The Role of Hsp70 in Conferring Unidirectionality on Protein Translocation into Mitochondria

10.1126/science.7973708

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The entry of segments of preproteins of defined lengths into the matrix space of mitochondria was studied. The mitochondrial chaperone Hsp70 (mtHsp70) interacted with proteins emerging from the protein import channel and stabilized translocation intermediates across the membranes in an adenosine triphosphate-dependent fashion. The chaperone bound to the presequence and mature parts of preproteins. In the absence of mtHsp70 binding, preproteins with less than 30 to 40 residues in the matrix diffused out of mitochondria. Thus, protein translocation was reversible up to a late stage. The import channels in both mitochondrial membranes constitute a passive pore that interacts weakly with polypeptide chains entering the matrix.

Bibliography

Ungermann, C., Neupert, W., & Cyr, D. M. (1994). The Role of Hsp70 in Conferring Unidirectionality on Protein Translocation into Mitochondria. Science, 266(5188), 1250â1253.

Authors 3

Christian Ungermann (first)
Walter Neupert (additional)
Douglas M. Cyr (additional)

References 30 Referenced 205

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10.1126/science.4048938

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:18 p.m.)
Indexed	2 weeks, 5 days ago (Aug. 5, 2025, 9 a.m.)
Issued	30 years, 9 months ago (Nov. 18, 1994)
Published	30 years, 9 months ago (Nov. 18, 1994)
Published Print	30 years, 9 months ago (Nov. 18, 1994)

Funders 0

None

BibTeX

@article{Ungermann_1994, title={The Role of Hsp70 in Conferring Unidirectionality on Protein Translocation into Mitochondria}, volume={266}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7973708}, DOI={10.1126/science.7973708}, number={5188}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Ungermann, Christian and Neupert, Walter and Cyr, Douglas M.}, year={1994}, month=nov, pages={1250–1253} }

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