DOI: 10.1126/science.7973650. Probing Individual Molecules with Confocal Fluorescence Microscopy

Probing Individual Molecules with Confocal Fluorescence Microscopy

10.1126/science.7973650

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Confocal fluorescence microscopy coupled with a diffraction-limited laser beam and a high-efficiency detection system has been used to study the diffusive movement and emission process of individual fluorescent molecules in the liquid phase at room temperature. The high detection sensitivity achieved at fast data acquisition speeds (greater than 1 kilohertz) allows real-time observation of single-molecule fluorescence without statistical analysis. The results show fluorescence-cycle saturation at the single-molecule level and multiple recrossings of a single molecule into and out of the probe volume as well as the triplet state.

Bibliography

Nie, S., Chiu, D. T., & Zare, R. N. (1994). Probing Individual Molecules with Confocal Fluorescence Microscopy. Science, 266(5187), 1018â1021.

Authors 3

Shuming Nie (first)
Daniel T. Chiu (additional)
Richard N. Zare (additional)

References 36 Referenced 482

10.1126/science.265.5170.364
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ISHIKAWA, M, JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS 33: 1571 (1994). / JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS (1994)
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NGUYEN, D.C., DETECTION OF SINGLE MOLECULES OF PHYCOERYTHRIN IN HYDRODYNAMICALLY FOCUSED FLOWS BY LASER-INDUCED FLUORESCENCE, ANALYTICAL CHEMISTRY 59: 2158 (1987). (10.1021/ac00144a032) / ANALYTICAL CHEMISTRY (1987)
ORRIT, M, HIGH-RESOLUTION SPECTROSCOPY OF ORGANIC-MOLECULES IN SOLIDS - FROM FLUORESCENCE LINE NARROWING AND HOLE-BURNING TO SINGLE-MOLECULE SPECTROSCOPY, JOURNAL OF PHYSICAL CHEMISTRY 97: 10256 (1993). (10.1021/j100142a003) / JOURNAL OF PHYSICAL CHEMISTRY (1993)
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RAMPINO, N.J., APPARATUS FOR GEL-ELECTROPHORESIS WITH CONTINUOUS MONITORING OF INDIVIDUAL DNA-MOLECULES BY VIDEO EPIFLUORESCENCE MICROSCOPY, ANALYTICAL BIOCHEMISTRY 194: 278 (1991). (10.1016/0003-2697(91)90230-Q) / ANALYTICAL BIOCHEMISTRY (1991)
RIGLER, R, FLUORESCENCE CORRELATION SPECTROSCOPY WITH HIGH COUNT RATE AND LOW-BACKGROUND - ANALYSIS OF TRANSLATIONAL DIFFUSION, EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 22: 169 (1993). / EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS (1993)
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10.1126/science.265.5170.361

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:22 p.m.)
Indexed	4 days, 6 hours ago (Aug. 27, 2025, 12:12 p.m.)
Issued	30 years, 9 months ago (Nov. 11, 1994)
Published	30 years, 9 months ago (Nov. 11, 1994)
Published Print	30 years, 9 months ago (Nov. 11, 1994)

Funders 0

None

BibTeX

@article{Nie_1994, title={Probing Individual Molecules with Confocal Fluorescence Microscopy}, volume={266}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7973650}, DOI={10.1126/science.7973650}, number={5187}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Nie, Shuming and Chiu, Daniel T. and Zare, Richard N.}, year={1994}, month=nov, pages={1018–1021} }

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