DOI: 10.1126/science.7973630. A Three-Dimensional Model for the Hammerhead Ribozyme Based on Fluorescence Measurements

A Three-Dimensional Model for the Hammerhead Ribozyme Based on Fluorescence Measurements

10.1126/science.7973630

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

For the understanding of the catalytic function of the RNA hammerhead ribozyme, a three-dimensional model is essential but neither a crystal nor a solution structure has been available. Fluorescence resonance energy transfer (FRET) was used to study the structure of the ribozyme in solution in order to establish the relative spatial orientation of the three constituent Watson-Crick base-paired helical segments. Synthetic constructs were labeled with the fluorescence donor (5-carboxyfluorescein) and acceptor (5-carboxytetramethylrhodamine) located at the ends of the strands constituting the ribozyme molecule. The acceptor helix in helix pairs I and III and in II and III was varied in length from 5 to 11 and 5 to 9 base pairs, respectively, and the FRET efficiencies were determined and correlated with a reference set of labeled RNA duplexes. The FRET efficiencies were predicted on the basis of vector algebra analysis, as a function of the relative helical orientations in the ribozyme constructs, and compared with experimental values. The data were consistent with a Y-shaped arrangement of the ribozyme with helices I and II in close proximity and helix III pointing away. These orientational constraints were used for molecular modeling of a three-dimensional structure of the complete ribozyme.

Bibliography

Tuschl, T., Gohlke, C., Jovin, T. M., Westhof, E., & Eckstein, F. (1994). A Three-Dimensional Model for the Hammerhead Ribozyme Based on Fluorescence Measurements. Science, 266(5186), 785â789.

Authors 5

Thomas Tuschl (first)
Christoph Gohlke (additional)
Thomas M. Jovin (additional)
Eric Westhof (additional)
Fritz Eckstein (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:16 p.m.)
Indexed	4 weeks ago (Aug. 6, 2025, 8:37 a.m.)
Issued	30 years, 9 months ago (Nov. 4, 1994)
Published	30 years, 9 months ago (Nov. 4, 1994)
Published Print	30 years, 9 months ago (Nov. 4, 1994)

Funders 0

None

BibTeX

@article{Tuschl_1994, title={A Three-Dimensional Model for the Hammerhead Ribozyme Based on Fluorescence Measurements}, volume={266}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7973630}, DOI={10.1126/science.7973630}, number={5186}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Tuschl, Thomas and Gohlke, Christoph and Jovin, Thomas M. and Westhof, Eric and Eckstein, Fritz}, year={1994}, month=nov, pages={785–789} }

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