DOI: 10.1126/science.7973628. Direct Measurement of the Forces Between Complementary Strands of DNA

Direct Measurement of the Forces Between Complementary Strands of DNA

10.1126/science.7973628

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Interaction forces between single strands of DNA were measured with the atomic force microscope by a procedure in which DNA oligonucleotides were covalently attached to a spherical probe and surface. Adhesive forces measured between complementary 20-base strands fell into three distinct distributions centered at 1.52, 1.11, and 0.83 nano-newtons, which are associated with the rupture of the interchain interaction between a single pair of molecules involving 20, 16, and 12 base pairs, respectively. When a third long DNA molecule was coupled between complementary surfaces, both intra- and interchain forces were observed. The intrachain interaction resulting from the molecule's elasticity manifested itself as a long-range cohesive force.

Bibliography

Lee, G. U., Chrisey, L. A., & Colton, R. J. (1994). Direct Measurement of the Forces Between Complementary Strands of DNA. Science, 266(5186), 771â773.

Authors 3

Gil U Lee (first)
Linda A. Chrisey (additional)
Richard J. Colton (additional)

References 28 Referenced 660

ALBRECHT, T.R., ATOMIC RESOLUTION WITH THE ATOMIC FORCE MICROSCOPE ON CONDUCTORS AND NONCONDUCTORS, JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A-VACUUM SURFACES AND FILMS 6: 271 (1988). (10.1116/1.575441) / JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A-VACUUM SURFACES AND FILMS (1988)
ASHKIN, A, OPTICAL TRAPPING AND MANIPULATION OF SINGLE CELLS USING INFRARED-LASER BEAMS, NATURE 330: 769 (1987). (10.1038/330769a0) / NATURE (1987)
BEAUCAGE, S.L., DEOXYNUCLEOSIDE PHOSPHORAMIDITES - A NEW CLASS OF KEY INTERMEDIATES FOR DEOXYPOLYNUCLEOTIDE SYNTHESIS, TETRAHEDRON LETTERS 22: 1859 (1981). (10.1016/S0040-4039(01)90461-7) / TETRAHEDRON LETTERS (1981)
BHATIA, S.K., USE OF THIOL-TERMINAL SILANES AND HETEROBIFUNCTIONAL CROSSLINKERS FOR IMMOBILIZATION OF ANTIBODIES ON SILICA SURFACES, ANALYTICAL BIOCHEMISTRY 178: 408 (1989). (10.1016/0003-2697(89)90662-3) / ANALYTICAL BIOCHEMISTRY (1989)
10.1073/pnas.83.11.3746
CHRISEY, L.A., MATERIALS RESEARCH SOCIETY SYMPOSIA PROCEEDINGS 330: 179 (1994). / MATERIALS RESEARCH SOCIETY SYMPOSIA PROCEEDINGS (1994)
CONNOLLY, B.A., CHEMICAL SYNTHESIS OF OLIGONUCLEOTIDES CONTAINING A FREE SULFHYDRYL-GROUP AND SUBSEQUENT ATTACHMENT OF THIOL SPECIFIC PROBES, NUCLEIC ACIDS RESEARCH 13: 4485 (1985). (10.1093/nar/13.12.4485) / NUCLEIC ACIDS RESEARCH (1985)
DENG, G.R., TERMINAL TRANSFERASE - USE IN THE TAILING OF DNA AND FOR INVITRO MUTAGENESIS, METHODS IN ENZYMOLOGY 100: 96 (1983). (10.1016/0076-6879(83)00047-6) / METHODS IN ENZYMOLOGY (1983)
DURIG, U, INTERACTION FORCE DETECTION IN SCANNING PROBE MICROSCOPY - METHODS AND APPLICATIONS, JOURNAL OF APPLIED PHYSICS 72: 1778 (1992). (10.1063/1.352348) / JOURNAL OF APPLIED PHYSICS (1992)
10.1126/science.8153628
Flory P. J. Principles of Polymer Chemistry (1953).
Flory P. J. Statistical Mechanics of Chain Molecules (1989).
INMAN, R.B., HELIX-RANDOM COIL TRANSITIONS IN DNA HOMOPOLYMER PAIRS, JOURNAL OF MOLECULAR BIOLOGY 8: 452 (1964). (10.1016/S0022-2836(64)80003-6) / JOURNAL OF MOLECULAR BIOLOGY (1964)
INMAN, R.B., TRANSITIONS OF DNA HOMOPOLYMERS, JOURNAL OF MOLECULAR BIOLOGY 9: 624 (1964). (10.1016/S0022-2836(64)80171-6) / JOURNAL OF MOLECULAR BIOLOGY (1964)
Israelachvili J. N. Intermolecular and Surface Forces 2 (1992).
JAMES, H.M., Theory of the elastic properties of rubber, JOURNAL OF CHEMICAL PHYSICS 11: 455 (1943). (10.1063/1.1723785) / JOURNAL OF CHEMICAL PHYSICS (1943)
Klump H. H. Biochemical Thermodynamics 2 105 (1988).
KUMAR, A, A SIMPLE METHOD FOR INTRODUCING A THIOL-GROUP AT THE 5'-END OF SYNTHETIC OLIGONUCLEOTIDES, NUCLEIC ACIDS RESEARCH 19: 4561 (1991). (10.1093/nar/19.16.4561) / NUCLEIC ACIDS RESEARCH (1991)
LEE, G.U., SENSING DISCRETE STREPTAVIDIN BIOTIN INTERACTIONS WITH ATOMIC-FORCE MICROSCOPY, LANGMUIR 10: 354 (1994). (10.1021/la00014a003) / LANGMUIR (1994)
MARKY, L.A., CALCULATING THERMODYNAMIC DATA FOR TRANSITIONS OF ANY MOLECULARITY FROM EQUILIBRIUM MELTING CURVES, BIOPOLYMERS 26: 1601 (1987). (10.1002/bip.360260911) / BIOPOLYMERS (1987)
MEYER, G, NOVEL OPTICAL APPROACH TO ATOMIC FORCE MICROSCOPY, APPLIED PHYSICS LETTERS 53: 1045 (1988). (10.1063/1.100061) / APPLIED PHYSICS LETTERS (1988)
10.1126/science.8171336
PUTMAN, CAJ, A DETAILED ANALYSIS OF THE OPTICAL BEAM DEFLECTION TECHNIQUE FOR USE IN ATOMIC FORCE MICROSCOPY, JOURNAL OF APPLIED PHYSICS 72: 6 (1992). (10.1063/1.352149) / JOURNAL OF APPLIED PHYSICS (1992)
Rau, D. C., Proceedings of the National Academy of Sciences of the United States of America 81: 2621 (1984). / Proceedings of the National Academy of Sciences of the United States of America (1984)
Saenger, W., Principles of Nucleic Acid Structure: CH6 (1983). / Principles of Nucleic Acid Structure (1983)
Sambrook J. Molecular Cloning: A Laboratory Manual (1989).
10.1126/science.1439819
10.1038/171737a0

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:16 p.m.)
Indexed	2 weeks, 6 days ago (Aug. 6, 2025, 9:41 a.m.)
Issued	30 years, 9 months ago (Nov. 4, 1994)
Published	30 years, 9 months ago (Nov. 4, 1994)
Published Print	30 years, 9 months ago (Nov. 4, 1994)

Funders 0

None

BibTeX

@article{Lee_1994, title={Direct Measurement of the Forces Between Complementary Strands of DNA}, volume={266}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7973628}, DOI={10.1126/science.7973628}, number={5186}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Lee, Gil U and Chrisey, Linda A. and Colton, Richard J.}, year={1994}, month=nov, pages={771–773} }

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