DOI: 10.1126/science.7939667. Restoration of X-ray Resistance and V(D)J Recombination in Mutant Cells by Ku cDNA

Restoration of X-ray Resistance and V(D)J Recombination in Mutant Cells by Ku cDNA

10.1126/science.7939667

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Three genetic complementation groups of rodent cells are defective for both repair of x-ray-induced double-strand breaks and V(D)J recombination. Cells from one group lack a DNA end-binding activity that is biochemically and antigenically similar to the Ku autoantigen. Transfection of complementary DNA (cDNA) that encoded the 86-kilodalton subunit of Ku rescued these mutant cells for DNA end-binding activity, x-ray resistance, and V(D)J recombination activity. These results establish a role for Ku in DNA repair and recombination. Furthermore, as a component of a DNA-dependent protein kinase, Ku may initiate a signaling pathway induced by DNA damage.

Bibliography

Smider, V., Rathmell, W. K., Lieber, M. R., & Chu, G. (1994). Restoration of X-ray Resistance and V(D)J Recombination in Mutant Cells by Ku cDNA. Science, 266(5183), 288â291.

Authors 4

Vaughn Smider (first)
W. Kimryn Rathmell (additional)
Michael R. Lieber (additional)
Gilbert Chu (additional)

References 27 Referenced 253

ANDERSON, C.W., DNA-DAMAGE AND THE DNA-ACTIVATED PROTEIN-KINASE, TRENDS IN BIOCHEMICAL SCIENCES 18: 433 (1993). (10.1016/0968-0004(93)90144-C) / TRENDS IN BIOCHEMICAL SCIENCES (1993)
BLIER, P.R., BINDING OF KU PROTEIN TO DNA - MEASUREMENT OF AFFINITY FOR ENDS AND DEMONSTRATION OF BINDING TO NICKS, JOURNAL OF BIOLOGICAL CHEMISTRY 268: 7594 (1993). (10.1016/S0021-9258(18)53216-6) / JOURNAL OF BIOLOGICAL CHEMISTRY (1993)
CAL, Q.Q., CYTOGENET CELL GENET 65: 221 (1994). (10.1159/000133635) / CYTOGENET CELL GENET (1994)
CHEN, D.J., REGIONAL ASSIGNMENT OF A HUMAN DNA-REPAIR GENE (XRCC5) TO 2Q35 BY X-RAY HYBRID MAPPING, GENOMICS 21: 423 (1994). (10.1006/geno.1994.1287) / GENOMICS (1994)
COLLINS, A.R., MUTANT RODENT CELL-LINES SENSITIVE TO ULTRAVIOLET-LIGHT, IONIZING-RADIATION AND CROSS-LINKING AGENTS - A COMPREHENSIVE SURVEY OF GENETIC AND BIOCHEMICAL CHARACTERISTICS, MUTATION RESEARCH 293: 99 (1993). (10.1016/0921-8777(93)90062-L) / MUTATION RESEARCH (1993)
FALZON, M, EBP-80, A TRANSCRIPTION FACTOR CLOSELY RESEMBLING THE HUMAN AUTOANTIGEN KU, RECOGNIZES SINGLE-STRAND TO DOUBLE-STRAND TRANSITIONS IN DNA, JOURNAL OF BIOLOGICAL CHEMISTRY 268: 10546 (1993). (10.1016/S0021-9258(18)82233-5) / JOURNAL OF BIOLOGICAL CHEMISTRY (1993)
Friedberg, E. C., The Molecular and Cellular Biology of the Yeast Saccharomyces 1 147 (1991). / The Molecular and Cellular Biology of the Yeast Saccharomyces (1991)
10.1016/S0021-9258(17)33960-1
10.1016/0042-6822(73)90341-3
GRIFFITH, A.J., KU POLYPEPTIDES SYNTHESIZED INVITRO ASSEMBLE INTO COMPLEXES WHICH RECOGNIZE ENDS OF DOUBLE-STRANDED DNA, JOURNAL OF BIOLOGICAL CHEMISTRY 267: 331 (1992). (10.1016/S0021-9258(18)48498-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1992)
10.1007/BF01233246
HAMILTON, A.A., GENE RECOMBINATION IN X-RAY-SENSITIVE HAMSTER-CELLS, MOLECULAR AND CELLULAR BIOLOGY 7: 1409 (1987). / MOLECULAR AND CELLULAR BIOLOGY (1987)
MIMORI, T, MECHANISM OF INTERACTION BETWEEN KU PROTEIN AND DNA, JOURNAL OF BIOLOGICAL CHEMISTRY 261: 10375 (1986). (10.1016/S0021-9258(18)67534-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1986)
MIMORI, T, CHARACTERIZATION OF A HIGH MOLECULAR-WEIGHT ACIDIC NUCLEAR-PROTEIN RECOGNIZED BY AUTOANTIBODIES IN SERA FROM PATIENTS WITH POLYMYOSITIS-SCLERODERMA OVERLAP, JOURNAL OF CLINICAL INVESTIGATION 68: 611 (1981). (10.1172/JCI110295) / JOURNAL OF CLINICAL INVESTIGATION (1981)
MIMORI, T, ISOLATION AND CHARACTERIZATION OF CDNA-ENCODING THE 80-KDA SUBUNIT PROTEIN OF THE HUMAN AUTOANTIGEN KU (P70/P80) RECOGNIZED BY AUTOANTIBODIES FROM PATIENTS WITH SCLERODERMA-POLYMYOSITIS OVERLAP SYNDROME, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 87: 1777 (1990). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1990)
MOORE, P.D., HOMOLOGOUS RECOMBINATION IN A CHINESE-HAMSTER X-RAY-SENSITIVE MUTANT, MUTATION RESEARCH 160: 149 (1986). (10.1016/0027-5107(86)90038-2) / MUTATION RESEARCH (1986)
MOROZOV, V.E., DNA-DEPENDENT PROTEIN-KINASE IS ACTIVATED BY NICKS AND LARGER SINGLE-STRANDED GAPS, JOURNAL OF BIOLOGICAL CHEMISTRY 269: 16684 (1994). (10.1016/S0021-9258(19)89444-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1994)
PERGOLA, F, V(D)J RECOMBINATION IN MAMMALIAN-CELL MUTANTS DEFECTIVE IN DNA DOUBLE-STRAND BREAK REPAIR, MOLECULAR AND CELLULAR BIOLOGY 13: 3464 (1993). / MOLECULAR AND CELLULAR BIOLOGY (1993)
RATHMELL, W.K., A DNA END-BINDING FACTOR INVOLVED IN DOUBLE-STRAND BREAK REPAIR AND V(D)J RECOMBINATION, MOLECULAR AND CELLULAR BIOLOGY 14: 4741 (1994). / MOLECULAR AND CELLULAR BIOLOGY (1994)
10.1073/pnas.91.16.7623
REEVES, W.H., MOLECULAR-CLONING OF CDNA-ENCODING THE P70 (KU) LUPUS AUTO-ANTIGEN, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 5047 (1989). (10.1016/S0021-9258(18)83697-3) / JOURNAL OF BIOLOGICAL CHEMISTRY (1989)
SOUTHERN, P.J., J MOL APPL GENET 1: 327 (1982). / J MOL APPL GENET (1982)
Taccioii, G. E., Current Topics in Microbiology and Immunology 182: 107 (1992). / Current Topics in Microbiology and Immunology (1992)
10.1126/science.8469973
TACCIOLI, G.E., KU80 - PRODUCT OF THE XRCC5 GENE AND ITS ROLE IN DNA-REPAIR AND V(D)J RECOMBINATION, SCIENCE 265: 1442 (1994). (10.1126/science.8073286) / SCIENCE (1994)
TAKABE, Y, MOL CELL BIOL 8: 466 (1988). / MOL CELL BIOL (1988)
YANEVA, M, CDNA-DERIVED AMINO-ACID SEQUENCE OF THE 86-KDA SUBUNIT OF THE KU ANTIGEN, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 13407 (1989). (10.1016/S0021-9258(18)80011-4) / JOURNAL OF BIOLOGICAL CHEMISTRY (1989)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:21 p.m.)
Indexed	2 weeks, 6 days ago (Aug. 6, 2025, 8 a.m.)
Issued	30 years, 10 months ago (Oct. 14, 1994)
Published	30 years, 10 months ago (Oct. 14, 1994)
Published Print	30 years, 10 months ago (Oct. 14, 1994)

Funders 0

None

BibTeX

@article{Smider_1994, title={Restoration of X-ray Resistance and V(D)J Recombination in Mutant Cells by Ku cDNA}, volume={266}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7939667}, DOI={10.1126/science.7939667}, number={5183}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Smider, Vaughn and Rathmell, W. Kimryn and Lieber, Michael R. and Chu, Gilbert}, year={1994}, month=oct, pages={288–291} }

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