DOI: 10.1126/science.7914032. Blockage of NF-κB Signaling by Selective Ablation of an mRNA target by 2-5A Antisense Chimeras

Blockage of NF-κB Signaling by Selective Ablation of an mRNA target by 2-5A Antisense Chimeras

10.1126/science.7914032

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Activation of 2-5A-dependent ribonuclease by 5′-phosphorylated, 2′,5′-linked oligoadenylates, known as 2-5A, is one pathway of interferon action. Unaided uptake into HeLa cells of 2-5A linked to an antisense oligonucleotide resulted in the selective ablation of messenger RNA for the double-stranded RNA (dsRNA)-dependent protein kinase PKR. Similarly, purified, recombinant human 2-5A-dependent ribonuclease was induced to selectively cleave PKR messenger RNA. Cells depleted of PKR activity were unresponsive to activation of nuclear factor-κB (NF-κB) by the dsRNA poly(I):poly(C), which provides direct evidence that PKR is a transducer for the dsRNA signaling of NF-κB.

Bibliography

Maran, A., Maitra, R. K., Kumar, A., Dong, B., Xiao, W., Li, G., Williams, B. R. G., Torrence, P. F., & Silverman, R. H. (1994). Blockage of NF-ÎºB Signaling by Selective Ablation of an mRNA target by 2-5A Antisense Chimeras. Science, 265(5173), 789â792.

Authors 9

Avudaiappan Maran (first)
Ratan K. Maitra (additional)
Aseem Kumar (additional)
Beihua Dong (additional)
Wei Xiao (additional)
Guiying Li (additional)
Bryan R. G. Williams (additional)
Paul F. Torrence (additional)
Robert H. Silverman (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 10:59 p.m.)
Indexed	2 months, 3 weeks ago (June 3, 2025, 12:26 a.m.)
Issued	31 years ago (Aug. 5, 1994)
Published	31 years ago (Aug. 5, 1994)
Published Print	31 years ago (Aug. 5, 1994)

Funders 0

None

BibTeX

@article{Maran_1994, title={Blockage of NF-κB Signaling by Selective Ablation of an mRNA target by 2-5A Antisense Chimeras}, volume={265}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7914032}, DOI={10.1126/science.7914032}, number={5173}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Maran, Avudaiappan and Maitra, Ratan K. and Kumar, Aseem and Dong, Beihua and Xiao, Wei and Li, Guiying and Williams, Bryan R. G. and Torrence, Paul F. and Silverman, Robert H.}, year={1994}, month=aug, pages={789–792} }

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