DOI: 10.1126/science.7871434. A p53-Dependent Mouse Spindle Checkpoint

A p53-Dependent Mouse Spindle Checkpoint

10.1126/science.7871434

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Cell cycle checkpoints enhance genetic fidelity by causing arrest at specific stages of the cell cycle when previous events have not been completed. The tumor suppressor p53 has been implicated in a G1 checkpoint. To investigate whether p53 also participates in a mitotic checkpoint, cultured fibroblasts from p53-deficient mouse embryos were exposed to spindle inhibitors. The fibroblasts underwent multiple rounds of DNA synthesis without completing chromosome segregation, thus forming tetraploid and octaploid cells. Deficiency of p53 was also associated with the development of tetraploidy in vivo. These results suggest that murine p53 is a component of a spindle checkpoint that ensures the maintenance of diploidy.

Bibliography

Cross, S. M., Sanchez, C. A., Morgan, C. A., Schimke, M. K., Ramel, S., Idzerda, R. L., Raskind, W. H., & Reid, B. J. (1995). A p53-Dependent Mouse Spindle Checkpoint. Science, 267(5202), 1353â1356.

Authors 8

Shawn M. Cross (first)
Carissa A. Sanchez (additional)
Catherine A. Morgan (additional)
Melana K. Schimke (additional)
Stig Ramel (additional)
Rejean L. Idzerda (additional)
Wendy H. Raskind (additional)
Brian J. Reid (additional)

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Dates

Type	When
Created	18 years, 9 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:24 p.m.)
Indexed	1 month, 1 week ago (July 16, 2025, 7:50 a.m.)
Issued	30 years, 5 months ago (March 3, 1995)
Published	30 years, 5 months ago (March 3, 1995)
Published Print	30 years, 5 months ago (March 3, 1995)

Funders 0

None

BibTeX

@article{Cross_1995, title={A p53-Dependent Mouse Spindle Checkpoint}, volume={267}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7871434}, DOI={10.1126/science.7871434}, number={5202}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Cross, Shawn M. and Sanchez, Carissa A. and Morgan, Catherine A. and Schimke, Melana K. and Ramel, Stig and Idzerda, Rejean L. and Raskind, Wendy H. and Reid, Brian J.}, year={1995}, month=mar, pages={1353–1356} }

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