DOI: 10.1126/science.7855599. Binding Strength Between Cell Adhesion Proteoglycans Measured by Atomic Force Microscopy

Binding Strength Between Cell Adhesion Proteoglycans Measured by Atomic Force Microscopy

10.1126/science.7855599

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Measurement of binding forces intrinsic to adhesion molecules is necessary to assess their contribution to the maintenance of the anatomical integrity of multicellular organisms. Atomic force microscopy was used to measure the binding strength between cell adhesion proteoglycans from a marine sponge. Under physiological conditions, the adhesive force between two cell adhesion molecules was found to be up to 400 piconewtons. Thus a single pair of molecules could hold the weight of 1600 cells. High intermolecular binding forces are likely to form the basis for the integrity of the multicellular sponge organism.

Bibliography

Dammer, U., Popescu, O., Wagner, P., Anselmetti, D., GÃ¼ntherodt, H.-J., & Misevic, G. N. (1995). Binding Strength Between Cell Adhesion Proteoglycans Measured by Atomic Force Microscopy. Science, 267(5201), 1173â1175.

Authors 6

Ulrich Dammer (first)
Octavian Popescu (additional)
Peter Wagner (additional)
Dario Anselmetti (additional)
Hans-Joachim Güntherodt (additional)
Gradimir N. Misevic (additional)

References 22 Referenced 356

ANSELMETTI, D, DOMAIN AND MOLECULAR SUPERLATTICE STRUCTURE OF DODECANETHIOL SELF-ASSEMBLED ON AU(111), EUROPHYSICS LETTERS 27: 365 (1994). (10.1209/0295-5075/27/5/006) / EUROPHYSICS LETTERS (1994)
BINNIG, G, ATOMIC FORCE MICROSCOPE, PHYSICAL REVIEW LETTERS 56: 930 (1986). (10.1103/PhysRevLett.56.930) / PHYSICAL REVIEW LETTERS (1986)
CAUDWELL, C.B., BIOCHEMISTRY-US 12: 3051 (1973). (10.1021/bi00740a017) / BIOCHEMISTRY-US (1973)
Dammer U. paper presented at the NATO ASI Workshop Schluchsee Germany 6 to 18 March (1994).
FINER, J.T., SINGLE MYOSIN MOLECULE MECHANICS - PICONEWTON FORCES AND NANOMETER STEPS, NATURE 368: 113 (1994). (10.1038/368113a0) / NATURE (1994)
10.1126/science.8153628
GUTIERREZ, C, PURIFICATION OF HUMAN T-CELLS AND B-CELLS BY A DISCONTINUOUS DENSITY GRADIENT OF PERCOLL, JOURNAL OF IMMUNOLOGICAL METHODS 29: 57 (1979). (10.1016/0022-1759(79)90125-X) / JOURNAL OF IMMUNOLOGICAL METHODS (1979)
Harmanson G. T. Immobilized Affinity Ligand Techniques (1992).
HELM, C.A., MEASUREMENT OF LIGAND RECEPTOR INTERACTIONS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 8169 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
HUMPHREYS, S, ORGANIZATION AND POLYSACCHARIDES OF SPONGE AGGREGATION FACTOR, JOURNAL OF SUPRAMOLECULAR STRUCTURE 7: 339 (1977). (10.1002/jss.400070307) / JOURNAL OF SUPRAMOLECULAR STRUCTURE (1977)
HUMPHREYS, T, CHEMICAL DISSOLUTION AND IN VITRO RECONSTRUCTION OF SPONGE CELL ADHESIONS .1. ISOLATION AND FUNCTIONAL DEMONSTRATION OF COMPONENTS INVOLVED, DEVELOPMENTAL BIOLOGY 8: 27 (1963). (10.1016/0012-1606(63)90024-1) / DEVELOPMENTAL BIOLOGY (1963)
JUMBLATT, J.E., CELL-CELL RECOGNITION - SPECIFIC BINDING OF MICROCIONA SPONGE AGGREGATION FACTOR TO HOMOTYPIC CELLS AND THE ROLE OF CALCIUM-IONS, BIOCHEMISTRY 19: 1038 (1980). (10.1021/bi00546a032) / BIOCHEMISTRY (1980)
LECKBAND, D.E., LONG-RANGE ATTRACTION AND MOLECULAR-REARRANGEMENTS IN RECEPTOR-LIGAND INTERACTIONS, SCIENCE 255: 1419 (1992). (10.1126/science.1542789) / SCIENCE (1992)
LEE, G.U., SENSING DISCRETE STREPTAVIDIN BIOTIN INTERACTIONS WITH ATOMIC-FORCE MICROSCOPY, LANGMUIR 10: 354 (1994). (10.1021/la00014a003) / LANGMUIR (1994)
MISEVIC, G.N., INVOLVEMENT OF CARBOHYDRATES AS MULTIPLE LOW AFFINITY INTERACTION SITES IN THE SELF-ASSOCIATION OF THE AGGREGATION FACTOR FROM THE MARINE SPONGE MICROCIONA-PROLIFERA, JOURNAL OF BIOLOGICAL CHEMISTRY 262: 5870 (1987). (10.1016/S0021-9258(18)45655-4) / JOURNAL OF BIOLOGICAL CHEMISTRY (1987)
MISEVIC, G.N., CARBOHYDRATE-CARBOHYDRATE INTERACTIONS OF A NOVEL ACIDIC GLYCAN CAN MEDIATE SPONGE CELL-ADHESION, JOURNAL OF BIOLOGICAL CHEMISTRY 268: 4922 (1993). (10.1016/S0021-9258(18)53484-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1993)
10.1126/science.8079171
RICE, D.J., 2 CA-2+ FUNCTIONS ARE DEMONSTRATED BY THE SUBSTITUTION OF SPECIFIC DIVALENT AND LANTHANIDE CATIONS FOR THE CA-2+ REQUIRED BY THE AGGREGATION FACTOR COMPLEX FROM THE MARINE SPONGE, MICROCIONA-PROLIFERA, JOURNAL OF BIOLOGICAL CHEMISTRY 258: 6394 (1983). (10.1016/S0021-9258(18)32422-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1983)
SPILLMANN, D, CHARACTERIZATION OF A NOVEL PYRUVYLATED CARBOHYDRATE UNIT IMPLICATED IN THE CELL-AGGREGATION OF THE MARINE SPONGE MICROCIONA-PROLIFERA, JOURNAL OF BIOLOGICAL CHEMISTRY 268: 13378 (1993). (10.1016/S0021-9258(19)38662-4) / JOURNAL OF BIOLOGICAL CHEMISTRY (1993)
Ulmann A. An Introduction to Ultrathin Organic Films (1991).
WAGNER P unpublished data.
WINTER, W.T., HYALURONIC-ACID - ROLE OF DIVALENT-CATIONS IN CONFORMATION AND PACKING, JOURNAL OF MOLECULAR BIOLOGY 117: 761 (1977). (10.1016/0022-2836(77)90068-7) / JOURNAL OF MOLECULAR BIOLOGY (1977)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:29 p.m.)
Indexed	3 weeks, 5 days ago (Aug. 7, 2025, 4:55 a.m.)
Issued	30 years, 6 months ago (Feb. 24, 1995)
Published	30 years, 6 months ago (Feb. 24, 1995)
Published Print	30 years, 6 months ago (Feb. 24, 1995)

Funders 0

None

BibTeX

@article{Dammer_1995, title={Binding Strength Between Cell Adhesion Proteoglycans Measured by Atomic Force Microscopy}, volume={267}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7855599}, DOI={10.1126/science.7855599}, number={5201}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Dammer, Ulrich and Popescu, Octavian and Wagner, Peter and Anselmetti, Dario and Güntherodt, Hans-Joachim and Misevic, Gradimir N.}, year={1995}, month=feb, pages={1173–1175} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        7
      ]
    ],
    "date-time": "2025-08-07T08:55:35Z",
    "timestamp": 1754556935400
  },
  "reference-count": 22,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "5201",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        2,
        24
      ]
    ]
  },
  "abstract": "<jats:p>Measurement of binding forces intrinsic to adhesion molecules is necessary to assess their contribution to the maintenance of the anatomical integrity of multicellular organisms. Atomic force microscopy was used to measure the binding strength between cell adhesion proteoglycans from a marine sponge. Under physiological conditions, the adhesive force between two cell adhesion molecules was found to be up to 400 piconewtons. Thus a single pair of molecules could hold the weight of 1600 cells. High intermolecular binding forces are likely to form the basis for the integrity of the multicellular sponge organism.</jats:p>",
  "DOI": "10.1126/science.7855599",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        27
      ]
    ],
    "date-time": "2006-10-27T18:19:42Z",
    "timestamp": 1161973182000
  },
  "page": "1173-1175",
  "source": "Crossref",
  "is-referenced-by-count": 356,
  "title": "Binding Strength Between Cell Adhesion Proteoglycans Measured by Atomic Force Microscopy",
  "prefix": "10.1126",
  "volume": "267",
  "author": [
    {
      "given": "Ulrich",
      "family": "Dammer",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Octavian",
      "family": "Popescu",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Peter",
      "family": "Wagner",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Dario",
      "family": "Anselmetti",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Hans-Joachim",
      "family": "G\u00fcntherodt",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Gradimir N.",
      "family": "Misevic",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_2_1_1_1",
      "doi-asserted-by": "crossref",
      "first-page": "365",
      "DOI": "10.1209/0295-5075/27/5/006",
      "volume": "27",
      "year": "1994",
      "unstructured": "ANSELMETTI, D, DOMAIN AND MOLECULAR SUPERLATTICE STRUCTURE OF DODECANETHIOL SELF-ASSEMBLED ON AU(111), EUROPHYSICS LETTERS 27: 365 (1994).",
      "journal-title": "EUROPHYSICS LETTERS"
    },
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "crossref",
      "first-page": "930",
      "DOI": "10.1103/PhysRevLett.56.930",
      "volume": "56",
      "year": "1986",
      "unstructured": "BINNIG, G, ATOMIC FORCE MICROSCOPE, PHYSICAL REVIEW LETTERS 56: 930 (1986).",
      "journal-title": "PHYSICAL REVIEW LETTERS"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "crossref",
      "first-page": "3051",
      "DOI": "10.1021/bi00740a017",
      "volume": "12",
      "year": "1973",
      "unstructured": "CAUDWELL, C.B., BIOCHEMISTRY-US 12: 3051 (1973).",
      "journal-title": "BIOCHEMISTRY-US"
    },
    {
      "key": "e_1_2_1_4_1",
      "unstructured": "Dammer U. paper presented at the NATO ASI Workshop Schluchsee Germany 6 to 18 March (1994)."
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "crossref",
      "first-page": "113",
      "DOI": "10.1038/368113a0",
      "volume": "368",
      "year": "1994",
      "unstructured": "FINER, J.T., SINGLE MYOSIN MOLECULE MECHANICS - PICONEWTON FORCES AND NANOMETER STEPS, NATURE 368: 113 (1994).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8153628"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "crossref",
      "first-page": "57",
      "DOI": "10.1016/0022-1759(79)90125-X",
      "volume": "29",
      "year": "1979",
      "unstructured": "GUTIERREZ, C, PURIFICATION OF HUMAN T-CELLS AND B-CELLS BY A DISCONTINUOUS DENSITY GRADIENT OF PERCOLL, JOURNAL OF IMMUNOLOGICAL METHODS 29: 57 (1979).",
      "journal-title": "JOURNAL OF IMMUNOLOGICAL METHODS"
    },
    {
      "key": "e_1_2_1_8_1",
      "unstructured": "Harmanson G. T. Immobilized Affinity Ligand Techniques (1992)."
    },
    {
      "key": "e_1_2_1_9_1",
      "first-page": "8169",
      "volume": "88",
      "year": "1991",
      "unstructured": "HELM, C.A., MEASUREMENT OF LIGAND RECEPTOR INTERACTIONS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 8169 (1991).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "crossref",
      "first-page": "339",
      "DOI": "10.1002/jss.400070307",
      "volume": "7",
      "year": "1977",
      "unstructured": "HUMPHREYS, S, ORGANIZATION AND POLYSACCHARIDES OF SPONGE AGGREGATION FACTOR, JOURNAL OF SUPRAMOLECULAR STRUCTURE 7: 339 (1977).",
      "journal-title": "JOURNAL OF SUPRAMOLECULAR STRUCTURE"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "crossref",
      "first-page": "27",
      "DOI": "10.1016/0012-1606(63)90024-1",
      "volume": "8",
      "year": "1963",
      "unstructured": "HUMPHREYS, T, CHEMICAL DISSOLUTION AND IN VITRO RECONSTRUCTION OF SPONGE CELL ADHESIONS .1. ISOLATION AND FUNCTIONAL DEMONSTRATION OF COMPONENTS INVOLVED, DEVELOPMENTAL BIOLOGY 8: 27 (1963).",
      "journal-title": "DEVELOPMENTAL BIOLOGY"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "crossref",
      "first-page": "1038",
      "DOI": "10.1021/bi00546a032",
      "volume": "19",
      "year": "1980",
      "unstructured": "JUMBLATT, J.E., CELL-CELL RECOGNITION - SPECIFIC BINDING OF MICROCIONA SPONGE AGGREGATION FACTOR TO HOMOTYPIC CELLS AND THE ROLE OF CALCIUM-IONS, BIOCHEMISTRY 19: 1038 (1980).",
      "journal-title": "BIOCHEMISTRY"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "crossref",
      "first-page": "1419",
      "DOI": "10.1126/science.1542789",
      "volume": "255",
      "year": "1992",
      "unstructured": "LECKBAND, D.E., LONG-RANGE ATTRACTION AND MOLECULAR-REARRANGEMENTS IN RECEPTOR-LIGAND INTERACTIONS, SCIENCE 255: 1419 (1992).",
      "journal-title": "SCIENCE"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "crossref",
      "first-page": "354",
      "DOI": "10.1021/la00014a003",
      "volume": "10",
      "year": "1994",
      "unstructured": "LEE, G.U., SENSING DISCRETE STREPTAVIDIN BIOTIN INTERACTIONS WITH ATOMIC-FORCE MICROSCOPY, LANGMUIR 10: 354 (1994).",
      "journal-title": "LANGMUIR"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "crossref",
      "first-page": "5870",
      "DOI": "10.1016/S0021-9258(18)45655-4",
      "volume": "262",
      "year": "1987",
      "unstructured": "MISEVIC, G.N., INVOLVEMENT OF CARBOHYDRATES AS MULTIPLE LOW AFFINITY INTERACTION SITES IN THE SELF-ASSOCIATION OF THE AGGREGATION FACTOR FROM THE MARINE SPONGE MICROCIONA-PROLIFERA, JOURNAL OF BIOLOGICAL CHEMISTRY 262: 5870 (1987).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "crossref",
      "first-page": "4922",
      "DOI": "10.1016/S0021-9258(18)53484-0",
      "volume": "268",
      "year": "1993",
      "unstructured": "MISEVIC, G.N., CARBOHYDRATE-CARBOHYDRATE INTERACTIONS OF A NOVEL ACIDIC GLYCAN CAN MEDIATE SPONGE CELL-ADHESION, JOURNAL OF BIOLOGICAL CHEMISTRY 268: 4922 (1993).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8079171"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "crossref",
      "first-page": "6394",
      "DOI": "10.1016/S0021-9258(18)32422-0",
      "volume": "258",
      "year": "1983",
      "unstructured": "RICE, D.J., 2 CA-2+ FUNCTIONS ARE DEMONSTRATED BY THE SUBSTITUTION OF SPECIFIC DIVALENT AND LANTHANIDE CATIONS FOR THE CA-2+ REQUIRED BY THE AGGREGATION FACTOR COMPLEX FROM THE MARINE SPONGE, MICROCIONA-PROLIFERA, JOURNAL OF BIOLOGICAL CHEMISTRY 258: 6394 (1983).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "crossref",
      "first-page": "13378",
      "DOI": "10.1016/S0021-9258(19)38662-4",
      "volume": "268",
      "year": "1993",
      "unstructured": "SPILLMANN, D, CHARACTERIZATION OF A NOVEL PYRUVYLATED CARBOHYDRATE UNIT IMPLICATED IN THE CELL-AGGREGATION OF THE MARINE SPONGE MICROCIONA-PROLIFERA, JOURNAL OF BIOLOGICAL CHEMISTRY 268: 13378 (1993).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_20_1",
      "unstructured": "Ulmann A. An Introduction to Ultrathin Organic Films (1991)."
    },
    {
      "key": "e_1_2_1_21_1",
      "unstructured": "WAGNER P unpublished data."
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "crossref",
      "first-page": "761",
      "DOI": "10.1016/0022-2836(77)90068-7",
      "volume": "117",
      "year": "1977",
      "unstructured": "WINTER, W.T., HYALURONIC-ACID - ROLE OF DIVALENT-CATIONS IN CONFORMATION AND PACKING, JOURNAL OF MOLECULAR BIOLOGY 117: 761 (1977).",
      "journal-title": "JOURNAL OF MOLECULAR BIOLOGY"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.7855599",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        15
      ]
    ],
    "date-time": "2024-01-15T04:29:33Z",
    "timestamp": 1705292973000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.7855599"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        2,
        24
      ]
    ]
  },
  "references-count": 22,
  "journal-issue": {
    "issue": "5201",
    "published-print": {
      "date-parts": [
        [
          1995,
          2,
          24
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.7855599"
  ],
  "URL": "http://dx.doi.org/10.1126/science.7855599",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1995,
        2,
        24
      ]
    ]
  }
}