DOI: 10.1126/science.7770774. Crystal Structure of a Purple Acid Phosphatase Containing a Dinuclear Fe(III)-Zn(II) Active Site

Crystal Structure of a Purple Acid Phosphatase Containing a Dinuclear Fe(III)-Zn(II) Active Site

10.1126/science.7770774

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Kidney bean purple acid phosphatase (KBPAP) is an Fe(III)-Zn(II) metalloenzyme resembling the mammalian Fe(III)-Fe(II) purple acid phosphatases. The structure of the homodimeric 111-kilodalton KBPAP was determined at a resolution of 2.9 angstroms. The enzyme contains two domains in each subunit. The active site is located in the carboxyl-terminal domain at the carboxy end of two sandwiched βαβαβ motifs. The two metal ions are 3.1 angstroms apart and bridged monodentately by Asp 164 . The iron is further coordinated by Tyr 167 , His 325 , and Asp 135 , and the zinc by His 286 , His 323 , and Asn 201 . The active-site structure is consistent with previous proposals regarding the mechanism of phosphate ester hydrolysis involving nucleophilic attack on the phosphate group by an Fe(III)-coordinated hydroxide ion.

Bibliography

StrÃ¤ter, N., Klabunde, T., Tucker, P., Witzel, H., & Krebs, B. (1995). Crystal Structure of a Purple Acid Phosphatase Containing a Dinuclear Fe(III)-Zn(II) Active Site. Science, 268(5216), 1489â1492.

Authors 5

Norbert Sträter (first)
Thomas Klabunde (additional)
Paul Tucker (additional)
Herbert Witzel (additional)
Bernt Krebs (additional)

References 28 Referenced 368

10.1107/S0907444994003112
Aquino, M. A. S., Journal of the Chemical Society, Dalton Transactions: 429 (1994). / Journal of the Chemical Society, Dalton Transactions (1994)
BECK, J. L., BIOCHIMICA ET BIOPHYSICA ACTA 869: 61 (1986). (10.1016/0167-4838(86)90310-9) / BIOCHIMICA ET BIOPHYSICA ACTA (1986)
10.1038/355472a0
Brunger A. T XPLOR Version 3.1 Manual (1993).
DIETRICH, M, PURPLE ACID-PHOSPHATASE FROM BOVINE SPLEEN - INTERACTIONS AT THE ACTIVE-SITE IN RELATION TO THE REACTION-MECHANISM, EUROPEAN JOURNAL OF BIOCHEMISTRY 199: 105 (1991). (10.1111/j.1432-1033.1991.tb16097.x) / EUROPEAN JOURNAL OF BIOCHEMISTRY (1991)
Doi, K., Structure and Bonding 70: 1 (1988). (10.1007/3-540-50130-4_1) / Structure and Bonding (1988)
HAYMAN, A.R., PURPLE ACID-PHOSPHATASE OF THE HUMAN MACROPHAGE AND OSTEOCLAST - CHARACTERIZATION, MOLECULAR-PROPERTIES, AND CRYSTALLIZATION OF THE RECOMBINANT DI-IRON-OXO PROTEIN SECRETED BY BACULOVIRUS-INFECTED INSECT CELLS, JOURNAL OF BIOLOGICAL CHEMISTRY 269: 1294 (1994). (10.1016/S0021-9258(17)42257-5) / JOURNAL OF BIOLOGICAL CHEMISTRY (1994)
HOLMES, M.A., STRUCTURES OF DEOXY AND OXY HEMERYTHRIN AT 2.0-A RESOLUTION, JOURNAL OF MOLECULAR BIOLOGY 218: 583 (1991). (10.1016/0022-2836(91)90703-9) / JOURNAL OF MOLECULAR BIOLOGY (1991)
HOLZ, R.C., NOESY STUDIES ON THE FEIIICOII ACTIVE-SITE OF THE PURPLE ACID-PHOSPHATASE UTEROFERRIN, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 114: 4434 (1992). (10.1021/ja00037a079) / JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (1992)
10.1107/S0108767390010224
Jones T. A. Molecular Replacement (1992).
KAUZLARICH, S.M., X-RAY ABSORPTION STUDIES OF THE PURPLE ACID-PHOSPHATASE FROM BEEF SPLEEN, INORGANIC CHEMISTRY 25: 2781 (1986). (10.1021/ic00236a025) / INORGANIC CHEMISTRY (1986)
KIM, E.E., REACTION-MECHANISM OF ALKALINE-PHOSPHATASE BASED ON CRYSTAL-STRUCTURES - 2-METAL ION CATALYSIS, JOURNAL OF MOLECULAR BIOLOGY 218: 449 (1991). (10.1016/0022-2836(91)90724-K) / JOURNAL OF MOLECULAR BIOLOGY (1991)
Klabunde, T., European Journal of Biochemistry 226: 369 (1994). (10.1111/j.1432-1033.1994.tb20061.x) / European Journal of Biochemistry (1994)
Kleywegt G. J. Acta Crystallographica Section D in press.
10.1107/S0021889891004399
MUELLER, E.G., PURPLE ACID-PHOSPHATASE - A DIIRON ENZYME THAT CATALYZES A DIRECT PHOSPHO GROUP TRANSFER TO WATER, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 115: 2974 (1993). (10.1021/ja00060a055) / JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (1993)
NORDLUND, P, 3-DIMENSIONAL STRUCTURE OF THE FREE-RADICAL PROTEIN OF RIBONUCLEOTIDE REDUCTASE, NATURE 345: 593 (1990). (10.1038/345593a0) / NATURE (1990)
PRIGGEMEYER, S, XAS INVESTIGATIONS ON THE IRON-ZINC CENTER OF PURPLE ACID-PHOSPHATASE FROM RED KIDNEY BEANS, INORGANIC CHEMISTRY 34: 1445 (1995). (10.1021/ic00110a024) / INORGANIC CHEMISTRY (1995)
QUE, L, PROGRESS IN INORGANIC CHEMISTRY 38: 97 (1990). / PROGRESS IN INORGANIC CHEMISTRY (1990)
ROSENZWEIG, A.C., CRYSTAL-STRUCTURE OF A BACTERIAL NONHEME IRON HYDROXYLASE THAT CATALYZES THE BIOLOGICAL OXIDATION OF METHANE, NATURE 366: 537 (1993). (10.1038/366537a0) / NATURE (1993)
STAHL, B, THE OLIGOSACCHARIDES OF THE FE(III)-ZN(II) PURPLE ACID-PHOSPHATASE OF THE RED KIDNEY BEAN - DETERMINATION OF THE STRUCTURE BY A COMBINATION OF MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY AND SELECTIVE ENZYMATIC DEGRADATION, EUROPEAN JOURNAL OF BIOCHEMISTRY 220: 321 (1994). (10.1111/j.1432-1033.1994.tb18628.x) / EUROPEAN JOURNAL OF BIOCHEMISTRY (1994)
STRATER, N, CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC DATA OF PURPLE ACID-PHOSPHATASE FROM RED KIDNEY BEAN, JOURNAL OF MOLECULAR BIOLOGY 224: 511 (1992). (10.1016/0022-2836(92)91012-E) / JOURNAL OF MOLECULAR BIOLOGY (1992)
SUERBAUM, H, ZN-EXCHANGE AND MOSSBAUER STUDIES ON THE [FE-FE] DERIVATIVES OF THE PURPLE ACID FE(III)-ZN(II)-PHOSPHATASE FROM KIDNEY BEANS, EUROPEAN JOURNAL OF BIOCHEMISTRY 214: 313 (1993). (10.1111/j.1432-1033.1993.tb17926.x) / EUROPEAN JOURNAL OF BIOCHEMISTRY (1993)
TRUE, A.E., EXAFS STUDIES OF UTEROFERRIN AND ITS ANION COMPLEXES, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 115: 4246 (1993). (10.1021/ja00063a047) / JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (1993)
VINCENT, J.B., CHEM REV 90: 1447 (1991). / CHEM REV (1991)
WANG, Z.G., H-1-NMR AND NOE STUDIES OF THE PURPLE ACID-PHOSPHATASES FROM PORCINE UTERUS AND BOVINE SPLEEN, BIOCHEMISTRY 31: 5263 (1992). (10.1021/bi00138a004) / BIOCHEMISTRY (1992)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:45 p.m.)
Indexed	2 weeks, 1 day ago (Aug. 12, 2025, 6:04 p.m.)
Issued	30 years, 2 months ago (June 9, 1995)
Published	30 years, 2 months ago (June 9, 1995)
Published Print	30 years, 2 months ago (June 9, 1995)

Funders 0

None

BibTeX

@article{Str_ter_1995, title={Crystal Structure of a Purple Acid Phosphatase Containing a Dinuclear Fe(III)-Zn(II) Active Site}, volume={268}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7770774}, DOI={10.1126/science.7770774}, number={5216}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Sträter, Norbert and Klabunde, Thomas and Tucker, Paul and Witzel, Herbert and Krebs, Bernt}, year={1995}, month=jun, pages={1489–1492} }

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    "date-parts": [
      [
        1995,
        6,
        9
      ]
    ]
  }
}