Distinct Binding Specificities and Functions of Higher Eukaryotic Polypyrimidine Tract-Binding Proteins
10.1126/science.7761834
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

In higher eukaryotes, the polypyrimidine-tract (Py-tract) adjacent to the 3′ splice site is recognized by several proteins, including the essential splicing factor U2AF 65 , the splicing regulator Sex-lethal (Sxl), and polypyrimidine tract-binding protein (PTB), whose function is unknown. Iterative in vitro genetic selection was used to show that these proteins have distinct sequence preferences. The uridine-rich degenerate sequences selected by U2AF 65 are similar to those present in the diverse array of natural metazoan Py-tracts. In contrast, the Sxl-consensus is a highly specific sequence, which can help explain the ability of Sxl to regulate splicing of transformer pre-mRNA and autoregulate splicing of its own pre-mRNA. The PTB-consensus is not a typical Py-tract; it can be found in certain alternatively spliced pre-mRNAs that undergo negative regulation. Here it is shown that PTB can regulate alternative splicing by selectively repressing 3′ splice sites that contain a PTB-binding site.

Bibliography

Singh, R., Valcárcel, J., & Green, M. R. (1995). Distinct Binding Specificities and Functions of Higher Eukaryotic Polypyrimidine Tract-Binding Proteins. Science, 268(5214), 1173–1176.

Authors 3
  1. Ravinder Singh (first)
  2. J Valcárcel (additional)
  3. Michael R. Green (additional)
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Dates
Type When
Created 18 years, 10 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited 1 year, 7 months ago (Jan. 14, 2024, 11:45 p.m.)
Indexed 1 week, 1 day ago (Aug. 19, 2025, 6:25 a.m.)
Issued 30 years, 3 months ago (May 26, 1995)
Published 30 years, 3 months ago (May 26, 1995)
Published Print 30 years, 3 months ago (May 26, 1995)
Funders 0

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@article{Singh_1995, title={Distinct Binding Specificities and Functions of Higher Eukaryotic Polypyrimidine Tract-Binding Proteins}, volume={268}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7761834}, DOI={10.1126/science.7761834}, number={5214}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Singh, Ravinder and Valcárcel, J and Green, Michael R.}, year={1995}, month=may, pages={1173–1176} }