DOI: 10.1126/science.7725106. In Vivo Functional Analysis of the Ras Exchange Factor Son of Sevenless

In Vivo Functional Analysis of the Ras Exchange Factor Son of Sevenless

10.1126/science.7725106

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The Son of sevenless (Sos) protein functions as a guanine nucleotide transfer factor for Ras and interacts with the receptor tyrosine kinase Sevenless through the protein Drk, a homolog of mammalian Grb2. In vivo structure-function analysis revealed that the amino terminus of Sos was essential for its function in flies. A molecule lacking the amino terminus was a potent dominant negative. In contrast, a Sos fragment lacking the Drk binding sites was functional and its activity was dependent on the presence of the Sevenless receptor. Furthermore, membrane localization of Sos was independent of Drk. A possible role for Drk as an activator of Sos is discussed and a Drk-independent interaction between Sos and Sevenless is proposed that is likely mediated by the pleckstrin homology domain within the amino terminus.

Bibliography

Karlovich, C. A., Bonfini, L., McCollam, L., Rogge, R. D., Daga, A., Czech, M. P., & Banerjee, U. (1995). In Vivo Functional Analysis of the Ras Exchange Factor Son of Sevenless. Science, 268(5210), 576â579.

Authors 7

Chris A. Karlovich (first)
Laura Bonfini (additional)
Linda McCollam (additional)
Ronald D. Rogge (additional)
Andrea Daga (additional)
Michael P. Czech (additional)
Utpal Banerjee (additional)

References 25 Referenced 62

ARONHEIM, A, MEMBRANE TARGETING OF THE NUCLEOTIDE EXCHANGE FACTOR SOS IS SUFFICIENT FOR ACTIVATING THE RAS SIGNALING PATHWAY, CELL 78: 949 (1994). (10.1016/0092-8674(94)90271-2) / CELL (1994)
BANERJEE, U, THE SEVENLESS+ PROTEIN IS EXPRESSED APICALLY IN CELL-MEMBRANES OF DEVELOPING DROSOPHILA RETINA - IT IS NOT RESTRICTED TO CELL R7, CELL 51: 151 (1987). (10.1016/0092-8674(87)90020-1) / CELL (1987)
BASLER, K, LIGAND-INDEPENDENT ACTIVATION OF THE SEVENLESS RECEPTOR TYROSINE KINASE CHANGES THE FATE OF CELLS IN THE DEVELOPING DROSOPHILA EYE, CELL 64: 1069 (1991). (10.1016/0092-8674(91)90262-W) / CELL (1991)
10.1126/science.1736363
BROEK, D, THE SACCHAROMYCES-CEREVISIAE CDC25 GENE-PRODUCT REGULATES THE RAS/ADENYLATE CYCLASE PATHWAY, CELL 48: 789 (1987). (10.1016/0092-8674(87)90076-6) / CELL (1987)
10.1016/0092-8674(93)90146-H
CLARK, S.G., C-ELEGANS CELL-SIGNALING GENE SEM-5 ENCODES A PROTEIN WITH SH2 AND SH3 DOMAINS, NATURE 356: 340 (1992). (10.1038/356340a0) / NATURE (1992)
FRANCESCHINI, N, PHENOMENA OF PSEUDOPUPIL IN COMPOUND EYE OF DROSOPHILA, KYBERNETIK 9: 159 (1971). (10.1007/BF02215177) / KYBERNETIK (1971)
HARLAN, J.E., PLECKSTRIN HOMOLOGY DOMAINS BIND TO PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE, NATURE 371: 168 (1994). (10.1038/371168a0) / NATURE (1994)
JONES, S, THE CDC25 PROTEIN OF SACCHAROMYCES-CEREVISIAE PROMOTES EXCHANGE OF GUANINE-NUCLEOTIDES BOUND TO RAS, MOLECULAR AND CELLULAR BIOLOGY 11: 2641 (1991). / MOLECULAR AND CELLULAR BIOLOGY (1991)
KARLOVICH C.A. unpublished data.
10.1038/352207a0
10.1016/0092-8674(92)90167-B
MCCOLLAM L unpublished data.
10.1016/0092-8674(93)90170-U
PAWSON, T, SH2 AND SH3 DOMAINS, CURRENT BIOLOGY 3: 434 (1993). (10.1016/0960-9822(93)90350-W) / CURRENT BIOLOGY (1993)
PERRIMON, N, SIGNALING PATHWAYS INITIATED BY RECEPTOR PROTEIN-TYROSINE KINASES IN DROSOPHILA, CURRENT OPINION IN CELL BIOLOGY 6: 260 (1994). (10.1016/0955-0674(94)90145-7) / CURRENT OPINION IN CELL BIOLOGY (1994)
ROGGE, R, NEURONAL DEVELOPMENT IN THE DROSOPHILA-RETINA - THE SEXTRA GENE DEFINES AN INHIBITORY COMPONENT IN THE DEVELOPMENTAL PATHWAY OF R7 PHOTORECEPTOR CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 89: 5271 (1992). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1992)
ROGGE, R.D., GENETIC DISSECTION OF A NEURODEVELOPMENTAL PATHWAY - SON OF SEVENLESS FUNCTIONS DOWNSTREAM OF THE SEVENLESS AND EGF RECEPTOR TYROSINE KINASES, CELL 64: 39 (1991). (10.1016/0092-8674(91)90207-F) / CELL (1991)
10.1016/0092-8674(91)90065-7
SIMON, M.A., AN SH3-SH2-SH3 PROTEIN IS REQUIRED FOR P21(RAS1) ACTIVATION AND BINDS TO SEVENLESS AND SOS PROTEINS INVITRO, CELL 73: 169 (1993). (10.1016/0092-8674(93)90169-Q) / CELL (1993)
STERNBERG, P.W., INTERCELLULAR SIGNALING AND SIGNAL-TRANSDUCTION IN C-ELEGANS, ANNUAL REVIEW OF GENETICS 27: 497 (1993). (10.1146/annurev.ge.27.120193.002433) / ANNUAL REVIEW OF GENETICS (1993)
TOUHARA, K, BINDING OF G-PROTEIN BETA-GAMMA-SUBUNITS TO PLECKSTRIN HOMOLOGY DOMAINS, JOURNAL OF BIOLOGICAL CHEMISTRY 269: 10217 (1994). (10.1016/S0021-9258(17)34048-6) / JOURNAL OF BIOLOGICAL CHEMISTRY (1994)
10.1016/0092-8674(90)90801-K
ZIPURSKY, S.L., DETERMINATION OF NEURONAL CELL FATE - LESSONS FROM THE R7 NEURON OF DROSOPHILA, ANNUAL REVIEW OF NEUROSCIENCE 17: 373 (1994). (10.1146/annurev.ne.17.030194.002105) / ANNUAL REVIEW OF NEUROSCIENCE (1994)

Dates

Type	When
Created	18 years, 9 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:44 p.m.)
Indexed	1 year, 7 months ago (Jan. 15, 2024, 7:03 p.m.)
Issued	30 years, 3 months ago (April 28, 1995)
Published	30 years, 3 months ago (April 28, 1995)
Published Print	30 years, 3 months ago (April 28, 1995)

Funders 0

None

BibTeX

@article{Karlovich_1995, title={In Vivo Functional Analysis of the Ras Exchange Factor Son of Sevenless}, volume={268}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7725106}, DOI={10.1126/science.7725106}, number={5210}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Karlovich, Chris A. and Bonfini, Laura and McCollam, Linda and Rogge, Ronald D. and Daga, Andrea and Czech, Michael P. and Banerjee, Utpal}, year={1995}, month=apr, pages={576–579} }

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